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LEUCOPERM Suspension fixation and permeabilization solution from GeneTex

ProductsLEUCOPERM Suspension fixation and permeabilization solution from GeneTex
Company GeneTex
Item LEUCOPERM Suspension fixation and permeabilization solution
Price $105.00
Description Reagent A - Fixation medium
Reagent B - Permeabilisation medium

LEUCOPERM reagents are intended for fixing cells in suspension with Reagent A and then permeabilising the cells with Reagent B. This procedure gives antibodies access to intracellular structures and leaves the morphological scatter characteristics of the cells intact. Specific formulations reduce background staining and allow simultaneous addition of permeabilisation medium and fluorochrome labelled antibodies. Flow cytometric analysis with monoclonal antibodies have been restricted primarily to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localisations of well established membrane molecules such as CD3 and CD22. LEUCOPERMTM reagents allow intracellular antigen analysis with the same ease as surface antigens. The only prerequisite is the availability of suitable antibody conjugates. Most commercially available monoclonal antibody conjugates can be used with LEUCOPERMTM reagents. Some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time may have to be determined experimentally for each antibody conjugate.

  • Form: Kit
Info GeneTexGeneTex
14785 Omicron Dr. Suite101
San Antonio, TX 78245
Customer Service: (877) 436-3839
Fax Number: (210) 677-8843
Web Site: http://www.genetex.com
NOTE: Price information is approximate list price and actual prices may vary.

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