Navigation Links
ImmunoPure IgM Purification Kit from Pierce Biotechnology, Inc.

ProductsImmunoPure IgM Purification Kit from Pierce Biotechnology, Inc.
Company Pierce Biotechnology, Inc.
Item ImmunoPure IgM Purification Kit
Price 
Description IgM binds poorly to Protein A and, until recently, no readily available affinity chromatography product existed for one- step IgM purification. Standard methods for IgM purification generally are multi-step, tedious processes, or they are not effective for removing all of the major impurities present in IgM samples. Pierce has developed an easy-to-use affinity matrix and buffer system to purify IgM. This matrix, an immobilized mannan binding protein (MBP) support, is most effective for purifying mouse IgM from ascites. Purified IgM can be obtained from a single pass over the affinity column. Human IgM will bind to the support and yield a product at least 88% pure by HPLC upon elution. The purification of IgMs from other species and mouse serum has not yet been optimized. Studies in Pierce labs show that MBP does not bind F(ab)2 and Fab. Mannan Binding Protein is immobilized on two supports: agarose and UltraLink Biosupport Medium. The ImmunoPure Immobilized MBP is also available in a kit format the ImmunoPure IgM Purification Kit. The kit contains sufficient buffers to perform 10 purifications using a 5 ml column of Immobilized MBP. Pierces ImmunoPure IgM Purification Kit is easy-to-use and yields 90% pure mouse IgM (from ascites) with a very simple protocol. Immobilized MBP can be regenerated at least 10 times with no apparent loss of binding capacity. The figure below demonstrates the high purity of Immobilized MBP purified IgM from mouse ascites.

  • Immobilized MBP Column 5 ml
  • IgM Binding Buffer 800 ml
  • IgM Elution Buffer 500 ml
  • MBP Column Preparation Buffer 50 ml
  • Column Extender
Info Pierce Biotechnology, Inc.Pierce Biotechnology, Inc.
Pierce Biotechnology, Inc.
3747 N. Meridian Rd.
P.O. Box 117
Rockford, IL 61105
Customer Service: 815-968-0747
Fax Number: 815-968-8148
Web Site: http://www.piercenet.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. STag Thrombin Purification Kit from Novagen
2. MagneGST™ Protein Purification System from Promega
3. PsiPort ™ Mass Directed Auto Purification Software from Shimadzu Biotech
4. DNA Spin Gel Extraction/PCR Purification Kit from Yorkshire Bioscience Ltd
5. MinElute 96 UF PCR Purification Kit (4) from QIAGEN
6. Rotofor Purification System, 100-120 V from Bio-Rad
7. Rotofor Purification System With Power Supply, 220-240 V from Bio-Rad
8. Protein Purification Plate Centrifuge compatible from ABgene
9. Acridinium Purification Buffer (10X) from Cayman Chemical
10. GST Expression and Purification Kit from BD Biosciences Pharmingen
11. Histidine-tagged Protein Purification Resin from R&D Systems
Identify and characterize Phosphoinositide (PI(4,5)P2) binding...
... Immunogen corresponds to amino acids 2-105 from ... been successfully used in Western blot, immunocytochemistry ... this antibody detects an ~ 60 kDa ... also been used in immunoprecipitation and immunocytochemical ...
Caspr2 Antigen: Fusion protein amino acids 96-1265 (extracellular domain) of human Caspr2 Accession Number: NP_054860...
... Our Accumix standard is a new tuning ... electrospray ionization mass spectrometry. Accumix is ... mass range coverage for both tuning and ... and LTQ-MS) and Fourier transform mass spectrometers. ...
Biology Products:
(Date:8/25/2014)... Wash.Five years ago this month, one of the first ... Washington State University campus, striking some 2,000 people. A ... of data gathered at the time to gain insight ... the virus,s rapid spread across the university community., The ... cases came in to the university,s Health and Wellness ...
(Date:8/25/2014)... battery-powered DNA diagnostic device invented at New Zealand,s ... commonly used field tool for rapidly detecting suspected ... the level of infection. , The breakthrough device, ... Queenstown Molecular Biology main meeting. It takes advantage ... target DNA sequences in real-time, without the need ...
(Date:8/25/2014)... a device the size of a cell phone may ... according to a team of engineers., "For biological testing ... said Tony Jun Huang, professor of engineering science and ... of the cells, damages them in any way, the ... waves can separate cells using very small amounts of ...
Breaking Biology News(10 mins):WSU flu outbreak provides rare study material 2WSU flu outbreak provides rare study material 3Revolutionary handheld DNA diagnostic unit allows lab-quality analysis in the field 2Tilted acoustic tweezers separate cells gently 2
... for many people with depression and anxiety disorders, and ... care providers, according to researchers who analyzed the results ... shown to have tremendous benefits for mental health," says ... Program at Southern Methodist University in Dallas. "The more ...
... at Ume University and the Sahlgrenska Academy at the University ... can be found in fish that have been swimming in ... found in higher concentrations in the blood of fish than ... this hormone can lead to infertility in fish. The ...
... neurodegenerative disease spinocerebellar ataxia type 5 (SCA5) damages nerve ... report that the defective protein responsible for the disease ... inside neurons. The study appears in the April 5 ... ( www.jcb.org ). SCA5 results from a faulty ...
Cached Biology News:Mental health providers should prescribe exercise more often for depression, anxiety 2Medicine residues may threaten fish reproduction 2Defective protein is a double hit for ataxia 2
(Date:8/26/2014)... San Francisco, CA (PRWEB) August 26, 2014 ... to reach USD 8,020.1 million by 2020, according to ... demand for personalized medicine and theranostics, and the subsequent ... be key factors driving market growth over the next ... population and chronic diseases such as cancer, coupled with ...
(Date:8/26/2014)... Genedata, a leading provider of ... science research, today announced the Genedata Screener® ... in Asia, Europe, and North America during September ... leading research organizations will gather for annual User ... and upcoming challenges in all areas of screening. ...
(Date:8/26/2014)... 26, 2014   Loveland Products, a subsidiary of ... crop input products and proprietary products provider for ... has acquired a controlling interest in Agricen, a ... delivering biochemical-based products for efficient and sustainable plant ... growing plant nutrition brands, Accomplish ® LM ...
(Date:8/26/2014)... -- That,s Nice LLC, a research-focused strategic marketing agency dedicated ... a new dedicated team that will support clients with ... current and new staff in both market research and ... Guy Tiene has been hired as Director ... previously Director of Marketing for Royal DSM,s Pharmaceutical Products ...
Breaking Biology Technology:World Molecular Diagnostics Market Is Expected To Grow To $8,020.1 Million From 2014 To 2020: Grand View Research, Inc 2World Molecular Diagnostics Market Is Expected To Grow To $8,020.1 Million From 2014 To 2020: Grand View Research, Inc 3World Molecular Diagnostics Market Is Expected To Grow To $8,020.1 Million From 2014 To 2020: Grand View Research, Inc 4World Molecular Diagnostics Market Is Expected To Grow To $8,020.1 Million From 2014 To 2020: Grand View Research, Inc 5World Molecular Diagnostics Market Is Expected To Grow To $8,020.1 Million From 2014 To 2020: Grand View Research, Inc 6Thought Leaders to Explore Innovations in SPR & Combination Screening at Genedata Screener User Group Meetings 2Thought Leaders to Explore Innovations in SPR & Combination Screening at Genedata Screener User Group Meetings 3Agrium, through Loveland Products, Acquires a Controlling Interest in Agricultural Biotechnology Company Agricen 2Agrium, through Loveland Products, Acquires a Controlling Interest in Agricultural Biotechnology Company Agricen 3Agrium, through Loveland Products, Acquires a Controlling Interest in Agricultural Biotechnology Company Agricen 4That's Nice Announces Expanded Offering For Strategic Content And Public Relations 2That's Nice Announces Expanded Offering For Strategic Content And Public Relations 3