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Immobilized Metal affinity ProteinChip Arrays from Ciphergen

ProductsImmobilized Metal affinity ProteinChip Arrays from Ciphergen
Company Ciphergen
Item Immobilized Metal affinity ProteinChip Arrays
Price 
Description Ciphergen's ProteinChip arrays have been developed for affinity capture of proteins from biological samples. A variety of ProteinChip chemical surfaces allow differential capture of proteins according to their unique biochemical properties. Each chip has eight different spots, allowing side-by-side sample comparison, and the chips are color-coded with a unique hydrophobic coating. Ciphergen's proprietary chip technology thus allows the researcher to rapidly capture, purify and characterize proteins directly on the chip.
Immobilized metal affinity capture (IMAC) arrays bind proteins and peptides which have affinity for metals; proteins with exposed histidine, tryptophan and/or cysteine typically bind to metals immobilized on these chip surfaces. Binding occurs under pH 6-8 and high salt and decreases as the concentration of imidizole and glycine increase.
ProteinChip IMAC3: contains a nitriloacetic acid (NTA) surface for high-capacity nickel binding and subsequent affinity capture of proteins with metal binding residues. Imidazole may be used in binding and washing solutions to moderate protein binding, including binding of non-specific proteins. Increasing the concentration of imidazole in the washing buffers reduces the binding of the target proteins.
Info CiphergenCiphergen
Ciphergen Biosystems, Inc
6611 Dumbarton Circle
Fremont, CA 94555

Customer Service: 510-505-2188
Fax Number: (510) 505-2101
Web Site: http://www.ciphergen.com
NOTE: Price information is approximate list price and actual prices may vary.

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Bethyl Laboratories packages antibodies, conjugates and calibrators to provide quantitative ELISA kits. Each kit contains the following components, sufficient for 1000 single well assays....
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... the optimization of immunostaining. ... the primary antibody, selection and ... Alk. Phos., HRP, fluorescence, or ... evaluation by light or fluorescence ...
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