Navigation Links
IMPACT-CN System from New England Biolabs

ProductsIMPACT-CN System from New England Biolabs
Company New England Biolabs
Item IMPACT-CN System
Price $400.00
Description IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) utilizes the inducible self-cleavage activity of engineered protein splicing elements (termed inteins) to purify recombinant proteins by a single affinity column (1-4) (Figures 1 and 2). This system distinguishes itself from other protein fusion systems by its ability to separate a recombinant protein from the affinity tag without the use of a protease.

The IMPACT-CN Protein Fusion and Purification System contains four expression vectors (pTYB vectors) which allow fusion of a bifunctional tag, consisting of the intein and the chitin binding domain (5), to either the C-terminus (pTYB1,2) or N-terminus (pTYB11,12) of the target protein. In the presence of thiols such as DTT, -mercaptoethanol or cysteine, the intein undergoes specific self-cleavage which releases the target protein from the chitin-bound intein tag.

Both pTYB1 and pTYB11 contain a Sap I cloning site which allows the target gene to be cloned immediately adjacent to the cleavage site of the intein tag; this results in the purification of a target protein without any extra non-native residues attached to its terminus after cleavage. Use of pTYB2 or pTYB12 yields a target protein with extra residue(s) added to its C-terminus or N-terminus, respectively. Addition of extra residues may help the cleavage reaction for some target proteins. pTYB2 and pTYB12 contain the same or compatible restriction sites in the multiple cloning region. This allows cloning the same target gene fragment into both vectors. pTYB1 and pTYB2 use ATG of the Nde I site in the multiple cloning region for translation initiation.

Info New England BiolabsNew England Biolabs
32 Tozer Rd.
Beverly, MA 01915-5599

Customer Service: 800-632-5227
Tech Support: 800-632-7799
Fax Number: 978-921-1350
Web Site: http://www.neb.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. R.A.P.I.D. System from Idaho Technology Inc.
2. CEQ 8800 Genetic Analysis System from Beckman Coulter
3. ProteomeLab PA 800 Protein Characterization System from Beckman Coulter
4. ProteomeLab PF 2D Protein Fractionation System from Beckman Coulter
5. pET GST Fusion System 42 plus Competent Cells from Novagen
6. pET GST Fusion System 42 from Novagen
7. pET GST Fusion System 41 plus Competent Cells from Novagen
8. pET GST Fusion System 41 from Novagen
9. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
10. CEQ 8000 Genetic Analysis System from Beckman Coulter
11. LCMS-IT-TOF Formula Predictor System from Shimadzu
... Rabbit polyclonal to Neuron navigator 1 ( ... Antigen: Synthetic peptide conjugated to KLH ... of Mouse Neuron navigator 1. (Note: the ... (Peptide available as ab32208 .) ...
pETDuet-1 is designed for the coexpression of two target genes....
Mouse Laminin-1 MAb (Clone AL-3)...
IEX-1 S/L (H-20)...
Biology Products:
(Date:3/30/2017)... The research team of The Hong Kong ... identification by adopting ground breaking 3D fingerprint minutiae recovery and matching ... and accuracy for use in identification, crime investigation, immigration control, security ... ... A research team led by ...
(Date:3/28/2017)... 28, 2017 The report "Video ... Monitors, Servers, Storage Devices), Software (Video Analytics, VMS), and ... Global Forecast to 2022", published by MarketsandMarkets, the market ... is projected to reach USD 75.64 Billion by 2022, ... The base year considered for the study is 2016 ...
(Date:3/24/2017)... Mar 24, 2017 Research and Markets has ... Market Analysis & Trends - Industry Forecast to 2025" report ... ... at a CAGR of around 15.1% over the next decade to ... report analyzes the market estimates and forecasts for all the given ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... BALTIMORE, Md. (PRWEB) , ... October 11, 2017 ... ... for digital pathology, announced today it will be hosting a Webinar titled, “Pathology ... of  Advanced Pathology Associates , on digital pathology adoption best practices and how ...
(Date:10/11/2017)... ... October 11, 2017 , ... ... granted orphan drug designation to SBT-100, its novel anti-STAT3 (Signal Transducer and Activator ... osteosarcoma. SBT-100 is able to cross the cell membrane and bind intracellular STAT3 ...
(Date:10/10/2017)... DALLAS , Oct. 10, 2017 International research firm ... IoT Strategy, will speak at the TMA 2017 Annual Meeting , ... key trends in the residential home security market and how smart safety ... ... "The ...
(Date:10/9/2017)... ... October 09, 2017 , ... The award-winning American Farmer ... first quarter 2018. American Farmer airs Tuesdays at 8:30aET on RFD-TV. , With ... with the challenge of how to continue to feed a growing nation. At the ...
Breaking Biology Technology: