Navigation Links
Human Topoisomerase II, Alpha from USB Corp.

ProductsHuman Topoisomerase II, Alpha from USB Corp.
Company USB Corp.
Item Human Topoisomerase II, Alpha
Price 
Description Source: E. coli containing a clone of the human Topoisomerase II gene.
Description:Topoisomerase II alters the topological state of nucleic acids by passing an intact DNA helix through a transient break which generates a separate DNA helix(1,2). As a result of its double-stranded DNA passage mechanism, the enzyme can relax negatively or positively supercoiled DNA, as well as catenate/decatenate or knot/unknot DNA molecules. Topoisomerase II has an absolute requirement for divalent cation and ATP (or dATP).
Tested User Friendly
Properties:
Molecular Weight: 340,000 Da homodimer
Optimum Temperature: 3037C
Optimum pH: 7.9, rapidly loses activity below pH 7.5
Requirements for Divalent Cation: 5mM Mg2+ is optimum for catalytic activity and 5mM Ca2+
 for DNA cleavage.
Optimum ATP Concentration: 0.5 to 1.0mM
Optimum Ion Strength: 100-170mM KCl/NaCl
Inhibitors: N-ethylmaleimide, zinc, novobicin, coumermycin A1, etoposide, amsacrine
Purity:Greater than 98% pure as determined by silver-stained SDS-PAGE. Free of contaminating exonucleases and endonucleases.
Storage Buffer:15mM sodium phosphate (pH 7.1), 700mM NaCl, 0.1mM EDTA, 0.5mM DTT, 50% glycerol.
Shipping and Storage:
Shipped on dry ice. Store at -20C for frequent use. Store at -80C for long-term storage.
Assay Conditions:The reaction mixture contains 10mM Tris-HCl, pH 7.9, 175mM KCl, 0.1mM EDTA, 5mM MgCl2, 2.5% glycerol, 0.5mM ATP.
Unit Definition:One unit is the amount of enzyme required to fully relax 0.3 g (5nM) of negatively supercoiled pBR322 plasmid DNA in 30 minutes at 37C under the standard assay conditions.
Concentration:20 units/l
Tested User FriendlyTM Functional Test: Tested for activity in standard Topoisomerase II unit assay.
Applications:*Relaxing negatively or positively supercoiled DNA.
*Catenating or decatenating DNA.
*Knotting or unknotting DNA.
PROTOCOL FOR RELAXATION OF SUPERCOILED DNA:
Topoisomerase II, Alpha and 10X Topoisomerase II Reaction Buffer (PN 73592) are functionally tested in the following protocol using 300 ng (0.1 pmol) pBR322 DNA at 30C.
*Mix
10X Topo II Reaction Buffer 2 l
Supercoiled DNA 0.11.0 pmol
Topoisomerase II, Alpha 110 units
H2O to 20 l
*Mix and incubate at 3037C for 15 minutes.
*Stop the reaction by the addition of 3 l of 7mM EDTA or by the addition of SDS
to 1%.
Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128


Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-216-245-1829
Customer Service: 216.765.5000
Fax Number: 216.464.5075
Web Site: http://www.usbweb.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Mouse Anti-Human S-100 alpha/beta chain Monoclonal Antibody, Unconjugated, Clone 8B10 from Santa Cruz Biotechnology, Inc.
2. Rabbit Anti-Human Vitamin K-dependent Protein S (PROS1) Polyclonal Antibody, Unconjugated from Lifespan Biosciences
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
5. Mouse Anti-Human Complement factor B Monoclonal Antibody, Unconjugated, Clone 9B6 from AntibodyShop A/S
6. Mouse Anti-Human Choriongonadotropin (hCG) Monoclonal Antibody, Unconjugated, Clone 5F10 from AntibodyShop A/S
7. Mouse Anti-Human Procollagen type I C-terminal propeptide (PICP) Monoclonal Antibody, Unconjugated from AntibodyShop A/S
8. Mouse Anti-Human Acetylcholinesterase, brain (AChE) Monoclonal Antibody, Unconjugated, Clone 12B4 from AntibodyShop A/S
9. Mouse Anti-Human a1-Antitrypsin (alpha-1 AT) Monoclonal Antibody, Unconjugated, Clone 4E5 from AntibodyShop A/S
10. pMIR-hU6-Luc, Human U6 Luciferase Control Vector from Mirus Bio Corporation
11. Human and Mouse RAR Neutralizing Peptide from ABR-Affinity BioReagents
Contains L-glutamine...
... does not share significant homology with ... is expressed in the central nervous ... stomach. One important role PEBP ... activity against several serine proteases including ...
...
Highly sensive assay for detecting apoptosis in living cells. See detailed descriptions above in K250-25....
Biology Products:
(Date:6/18/2013)... , June 19, 2013  Paris Air ... as Geotest-Marvin Test Systems) , a leading provider ... aerospace industries, is demonstrating its solutions this week ... booth 3-C82. With growing challenges including increasingly complex ... demanding efficient, feature-rich, specialized aerospace test solutions that ...
(Date:6/18/2013)... of Medicine have discovered a mechanism that is used ... component of blood typically associated with clotting, were discovered ... seal it off from the rest of the body. ... this week, provide the science community with a ... known that platelets do participate in immunity, but now ...
(Date:6/18/2013)... Department,s National Renewable Energy Laboratory (NREL), and the ... describing a novel cellulose-degrading enzyme from a marine ... as the gribble. , Gribbles are biologically ... to produce their own enzymes instead of using ... eat. New biomass-degrading enzymes from novel sources such ...
Breaking Biology News(10 mins):Marvin Test Solutions Demonstrates Next-Generation Armament Test Solutions at Paris Air Show 2Marvin Test Solutions Demonstrates Next-Generation Armament Test Solutions at Paris Air Show 3Immunity mechanism discovered 2Novel enzyme from tiny gribble could prove a boon for biofuels research 2
... Many would assume that "mounting an immune response" or ... thing. Now, research at the University of California, ... inflammation associated with the progression of tumors actually plays ... , The research, appearing online March 19 in advance ...
... eco-friendly, clean-burning fuel. But if every vehicle in the ... instead of pure gasoline, the number of respiratory-related deaths ... study by Stanford University atmospheric scientist Mark Z. Jacobson. ... edition of the journal Environmental Science & Technology (ES&T). ...
... the world's most endangered cat, the Amur or Far Eastern ... to 34 are left in the wild, renewing fears for ... World Wildlife Fund along with the Wildlife Conservation Society and ... Science, conducted a routine snow-track census of leopard numbers. ...
Cached Biology News:Inflammation may play role in metastasis of prostate cancer 2Inflammation may play role in metastasis of prostate cancer 3Ethanol vehicles pose a significant risk to human health, study finds 2Ethanol vehicles pose a significant risk to human health, study finds 3Amur leopard still on the brink of extinction, scientists say 2
(Date:6/18/2013)... , June 18, 2013  Techne Corporation (NASDAQ: ... it has agreed to acquire 100% ownership of ... Inc. (Bionostics).  Bionostics is a global leader in ... that verify the proper operation of in ... point of care blood glucose and blood gas ...
(Date:6/18/2013)... 2013 As a leading provider ... announces the release of their infographic - " ... , MeMD's goal was to leverage an information ... 2.0, Health 3.0 and mHealth.The infographic features a ... care and the fitness industry in unimaginable ways; ...
(Date:6/18/2013)... (PRWEB) June 18, 2013 ... automated system for rapid bacterial strain typing, announced ... joined the company as Vice President of Engineering. ... for the company’s RESOLUTION™ Microbial Genotyping System ... the benchtop instrument, pathogen-specific assay kits, database and ...
(Date:6/18/2013)... June 18, 2013 Kitware , ... announces a new Fast-Track award from the U.S. Department ... environment (IDAE) for the nuclear power industry. , Construction ... more than 30 years, but the United States’ nuclear ... gains in power plant utilization through improved refueling, maintenance, ...
Breaking Biology Technology:Techne Corporation Announces Acquisition Agreement 2Techne Corporation Announces Acquisition Agreement 3Techne Corporation Announces Acquisition Agreement 4Techne Corporation Announces Acquisition Agreement 5MeMD, Inc Releases Infographic Featuring the Top Eight Technologies that are Revolutionizing Health and Fitness Today 2PathoGenetix Hires Technology Development VP for Resolution Microbial Genotyping System 2PathoGenetix Hires Technology Development VP for Resolution Microbial Genotyping System 3Kitware to Develop an Open-Source HPC Design-Analysis Environment for Nuclear Energy Research 2