Navigation Links
Human Topoisomerase II, Alpha from USB Corp.

ProductsHuman Topoisomerase II, Alpha from USB Corp.
Company USB Corp.
Item Human Topoisomerase II, Alpha
Price 
Description Source: E. coli containing a clone of the human Topoisomerase II gene.
Description:Topoisomerase II alters the topological state of nucleic acids by passing an intact DNA helix through a transient break which generates a separate DNA helix(1,2). As a result of its double-stranded DNA passage mechanism, the enzyme can relax negatively or positively supercoiled DNA, as well as catenate/decatenate or knot/unknot DNA molecules. Topoisomerase II has an absolute requirement for divalent cation and ATP (or dATP).
Tested User Friendly
Properties:
Molecular Weight: 340,000 Da homodimer
Optimum Temperature: 3037C
Optimum pH: 7.9, rapidly loses activity below pH 7.5
Requirements for Divalent Cation: 5mM Mg2+ is optimum for catalytic activity and 5mM Ca2+
for DNA cleavage.
Optimum ATP Concentration: 0.5 to 1.0mM
Optimum Ion Strength: 100-170mM KCl/NaCl
Inhibitors: N-ethylmaleimide, zinc, novobicin, coumermycin A1, etoposide, amsacrine
Purity:Greater than 98% pure as determined by silver-stained SDS-PAGE. Free of contaminating exonucleases and endonucleases.
Storage Buffer:15mM sodium phosphate (pH 7.1), 700mM NaCl, 0.1mM EDTA, 0.5mM DTT, 50% glycerol.
Shipping and Storage:
Shipped on dry ice. Store at -20C for frequent use. Store at -80C for long-term storage.
Assay Conditions:The reaction mixture contains 10mM Tris-HCl, pH 7.9, 175mM KCl, 0.1mM EDTA, 5mM MgCl2, 2.5% glycerol, 0.5mM ATP.
Unit Definition:One unit is the amount of enzyme required to fully relax 0.3 g (5nM) of negatively supercoiled pBR322 plasmid DNA in 30 minutes at 37C under the standard assay conditions.
Concentration:20 units/l
Tested User FriendlyTM Functional Test: Tested for activity in standard Topoisomerase II unit assay.
Applications:*Relaxing negatively or positively supercoiled DNA.
*Catenating or decatenating DNA.
*Knotting or unknotting DNA.
PROTOCOL FOR RELAXATION OF SUPERCOILED DNA:
Topoisomerase II, Alpha and 10X Topoisomerase II Reaction Buffer (PN 73592) are functionally tested in the following protocol using 300 ng (0.1 pmol) pBR322 DNA at 30C.
*Mix
10X Topo II Reaction Buffer 2 l
Supercoiled DNA 0.11.0 pmol
Topoisomerase II, Alpha 110 units
H2O to 20 l
*Mix and incubate at 3037C for 15 minutes.
*Stop the reaction by the addition of 3 l of 7mM EDTA or by the addition of SDS
to 1%.
Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128


Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-216-245-1829
Customer Service: 216.765.5000
Fax Number: 216.464.5075
Web Site: http://www.usbweb.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Mouse Anti-Human S-100 alpha/beta chain Monoclonal Antibody, Unconjugated, Clone 8B10 from Santa Cruz Biotechnology, Inc.
2. Rabbit Anti-Human Vitamin K-dependent Protein S (PROS1) Polyclonal Antibody, Unconjugated from Lifespan Biosciences
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
5. Mouse Anti-Human Complement factor B Monoclonal Antibody, Unconjugated, Clone 9B6 from AntibodyShop A/S
6. Mouse Anti-Human Choriongonadotropin (hCG) Monoclonal Antibody, Unconjugated, Clone 5F10 from AntibodyShop A/S
7. Mouse Anti-Human Procollagen type I C-terminal propeptide (PICP) Monoclonal Antibody, Unconjugated from AntibodyShop A/S
8. Mouse Anti-Human Acetylcholinesterase, brain (AChE) Monoclonal Antibody, Unconjugated, Clone 12B4 from AntibodyShop A/S
9. Mouse Anti-Human a1-Antitrypsin (alpha-1 AT) Monoclonal Antibody, Unconjugated, Clone 4E5 from AntibodyShop A/S
10. pMIR-hU6-Luc, Human U6 Luciferase Control Vector from Mirus Bio Corporation
11. Human and Mouse RAR Neutralizing Peptide from ABR-Affinity BioReagents
... Immunogen: Synthetic peptide derived from the ... mouse protein kinase C zeta isoform (PKCzeta). ... mouse PKCzeta protein. Recognizes PKCzeta and ... with PKCiota/lambda. Reactivity: Mouse Rat (positive ...
Request Info...
Streptomycin sulfate an aminoglycoside used for bacterial selection binds to the small ribosomal subunit and interferes with protein biosynthesis.21H39N7O12)2x3H2SO4Formula weight:...
...
Biology Products:
(Date:1/25/2016)...  Glencoe Software, the world-leading supplier of image data ... provide the data management solution OMERO Plus for the ... Photo - http://photos.prnewswire.com/prnh/20160125/325328LOGO ... Phenotypic analysis measures the characteristics and behavior of ... states such as health and disease, the presence or ...
(Date:1/21/2016)... --> ... report "Emotion Detection and Recognition Market by Technology (Bio-Sensors, NLP, ... Voice Recognition and Others), Services, Application Areas, End ... published by MarketsandMarkets, the global Emotion Detection and ... Billion by 2020, at a CAGR of 31.9%, ...
(Date:1/20/2016)... SAN JOSE, Calif. , Jan. 20, 2016 ... leading developer of human interface solutions, today announced ... touch controller solution for wearables and small screen ... appliances such as printers. Supporting round and rectangular ... the S1423 offers excellent performance with moisture on ...
Breaking Biology News(10 mins):
(Date:2/9/2016)... ... February 09, 2016 , ... Tunnell Consulting, Inc. announced that ... Paris, he will focus on acquiring new accounts and work closely with existing Tunnell ... , “Fred brings to our European clients more than 15 years of experience in ...
(Date:2/9/2016)... ... February 09, 2016 , ... PharmApprove ... for the National Organization for Rare Disorders (NORD). Dorman will lead PharmApprove efforts ... are heard throughout the drug regulatory review process. , “Adding Diane Dorman is ...
(Date:2/9/2016)... LONDON , February 9, 2016 ... replace paper and protect IP   E-WorkBook ... will be rolled out in Germany ... and protect valuable IP. Users will be able to search ... or experiment as part of the application, to boost collaboration ...
(Date:2/9/2016)... Feb. 9, 2016 ... 2016", report provides in depth insights on ... around the Protein-Tyrosine Phosphatase 1B (PTP1B) Inhibitors. ... in various stages of development including Discovery, ... III and Preregistration. Report covers the product ...
Breaking Biology Technology: