Navigation Links
Human Topoisomerase II, Alpha from USB Corp.

ProductsHuman Topoisomerase II, Alpha from USB Corp.
Company USB Corp.
Item Human Topoisomerase II, Alpha
Price 
Description Source: E. coli containing a clone of the human Topoisomerase II gene.
Description:Topoisomerase II alters the topological state of nucleic acids by passing an intact DNA helix through a transient break which generates a separate DNA helix(1,2). As a result of its double-stranded DNA passage mechanism, the enzyme can relax negatively or positively supercoiled DNA, as well as catenate/decatenate or knot/unknot DNA molecules. Topoisomerase II has an absolute requirement for divalent cation and ATP (or dATP).
Tested User Friendly
Properties:
Molecular Weight: 340,000 Da homodimer
Optimum Temperature: 3037C
Optimum pH: 7.9, rapidly loses activity below pH 7.5
Requirements for Divalent Cation: 5mM Mg2+ is optimum for catalytic activity and 5mM Ca2+
for DNA cleavage.
Optimum ATP Concentration: 0.5 to 1.0mM
Optimum Ion Strength: 100-170mM KCl/NaCl
Inhibitors: N-ethylmaleimide, zinc, novobicin, coumermycin A1, etoposide, amsacrine
Purity:Greater than 98% pure as determined by silver-stained SDS-PAGE. Free of contaminating exonucleases and endonucleases.
Storage Buffer:15mM sodium phosphate (pH 7.1), 700mM NaCl, 0.1mM EDTA, 0.5mM DTT, 50% glycerol.
Shipping and Storage:
Shipped on dry ice. Store at -20C for frequent use. Store at -80C for long-term storage.
Assay Conditions:The reaction mixture contains 10mM Tris-HCl, pH 7.9, 175mM KCl, 0.1mM EDTA, 5mM MgCl2, 2.5% glycerol, 0.5mM ATP.
Unit Definition:One unit is the amount of enzyme required to fully relax 0.3 g (5nM) of negatively supercoiled pBR322 plasmid DNA in 30 minutes at 37C under the standard assay conditions.
Concentration:20 units/l
Tested User FriendlyTM Functional Test: Tested for activity in standard Topoisomerase II unit assay.
Applications:*Relaxing negatively or positively supercoiled DNA.
*Catenating or decatenating DNA.
*Knotting or unknotting DNA.
PROTOCOL FOR RELAXATION OF SUPERCOILED DNA:
Topoisomerase II, Alpha and 10X Topoisomerase II Reaction Buffer (PN 73592) are functionally tested in the following protocol using 300 ng (0.1 pmol) pBR322 DNA at 30C.
*Mix
10X Topo II Reaction Buffer 2 l
Supercoiled DNA 0.11.0 pmol
Topoisomerase II, Alpha 110 units
H2O to 20 l
*Mix and incubate at 3037C for 15 minutes.
*Stop the reaction by the addition of 3 l of 7mM EDTA or by the addition of SDS
to 1%.
Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128


Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-216-245-1829
Customer Service: 216.765.5000
Fax Number: 216.464.5075
Web Site: http://www.usbweb.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Mouse Anti-Human S-100 alpha/beta chain Monoclonal Antibody, Unconjugated, Clone 8B10 from Santa Cruz Biotechnology, Inc.
2. Rabbit Anti-Human Vitamin K-dependent Protein S (PROS1) Polyclonal Antibody, Unconjugated from Lifespan Biosciences
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
5. Mouse Anti-Human Complement factor B Monoclonal Antibody, Unconjugated, Clone 9B6 from AntibodyShop A/S
6. Mouse Anti-Human Choriongonadotropin (hCG) Monoclonal Antibody, Unconjugated, Clone 5F10 from AntibodyShop A/S
7. Mouse Anti-Human Procollagen type I C-terminal propeptide (PICP) Monoclonal Antibody, Unconjugated from AntibodyShop A/S
8. Mouse Anti-Human Acetylcholinesterase, brain (AChE) Monoclonal Antibody, Unconjugated, Clone 12B4 from AntibodyShop A/S
9. Mouse Anti-Human a1-Antitrypsin (alpha-1 AT) Monoclonal Antibody, Unconjugated, Clone 4E5 from AntibodyShop A/S
10. pMIR-hU6-Luc, Human U6 Luciferase Control Vector from Mirus Bio Corporation
11. Human and Mouse RAR Neutralizing Peptide from ABR-Affinity BioReagents
Plasmid expressing PLAP reporter gene....
Plasmid expressing the LacZ reporter gene....
Human SEAP ORF, provided in a pORF plasmid. pORF is an expression plasmid selectable with Ampicillin....
... culture flasks have triple baffles located at ... achieve maximal oxygen transfer to culture medium. ... A wide range of optional caps are ... colored caps for ease of sorting and ...
Biology Products:
(Date:12/10/2018)... ... December 10, 2018 , ... Today, RoosterBio Inc. and Tissue ... of accelerating the development of the Regenerative Medicine field. This alliance will ... around human umbilical cord perivascular cells (HUCPVCs). Together, it is anticipated that ...
(Date:12/5/2018)... ... December 03, 2018 , ... uBiome, the ... assistant professor in the Division of Gastroenterology and Hepatology at UC San Diego ... Advisory Board. Joining the board of leading scientists and doctors from around the ...
(Date:12/5/2018)... (PRWEB) , ... December 04, 2018 , ... ... has announced the much-anticipated launch of their 100-liter (26 gallon) SAFEthread Drum. ... Drum in 2017, which brought a much-needed modern redesign to the plastic ...
Breaking Biology News(10 mins):
(Date:11/9/2018)... (PRWEB) , ... November 08, 2018 , ... ... Dr. Pablo Valenzuela, PhD, co-Founder, Chief Executive Officer and President of Andes Biotechnologies, ... from around the world, Dr. Valenzuela brings to uBiome expertise on the genetic ...
(Date:11/9/2018)... PHILADELPHIA (PRWEB) , ... November 08, 2018 , ... ... system integrator of custom industrial automation and IT solutions with 140 employees on ... at Automation Fair®, taking place at the Pennsylvania Convention Center on November 14 ...
(Date:11/7/2018)... ... November 07, 2018 , ... Diversified Technologies, Inc. (DTI) has ... Arizona Center for Algae Technology and Innovation at ASU. Funded by a USDA NIFA ... those involved with algal and predator control applications. , Algae ponds get up to ...
(Date:11/5/2018)... ... November 05, 2018 , ... Frederick National Laboratory for Cancer ... to science research and education in the Frederick County area. , Frederick ... access to STEM-based undergraduate and graduate degree and certificate programs offered by partnering ...
Breaking Biology Technology: