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HRV 3C Protease from Novagen

ProductsHRV 3C Protease from Novagen
Company Novagen
Item HRV 3C Protease
Price $171.00
Description Recombinant type 14 3C protease from human rhinovirus (HRV 3C) is the newest addition to the Novagen brand line of restriction grade proteases. The recombinant protease is a highly purified recombinant 6XHis-fusion protein, which recognizes the same cleavage site as the native enzyme: LeuGluValLeuPheGln/GlyPro. The small, 22 KDa size of the protease, optimal activity at 4° C, high specificity, and His•Tag® fusion make HRV 3C protease an ideal choice for rapid removal of fusion tags. The pET expression vectors pET-47b(+) through pET-50b(+) incorporate the HRV 3C protease cleavage site in combinations with His•Tag, S•Tag, thioredoxin (Trx•Tag™), glutathione-S-transferase (GST•Tag™), and NusA (Nus•Tag™) sequences. The combination of pET-47b(+) to pET-50b(+) vectors for expression, HRV 3C protease for fusion tag cleavage, and Ni-NTA His•Bind® metal affinity chromatography for protein purification as well as protease and fusion tag removal allows production of recombinant proteins free of vector-encoded sequences. Supplied as: 2000 units/ml in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.

kit_contents:
  • HRV 3C Protease
  • HRV 3C Cleavage Control Protein
  • 10X HRV 3C Cleavage Buffer.


formulation:
2000 units/ml in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.
Info NovagenNovagen
441 Charmany Drive
Madison, WI 53719
Customer Service: 800-526-7319
Tech Support: 800-207-0144
Fax Number: 608-238-1388
Web Site: http://www.novagen.com
NOTE: Price information is approximate list price and actual prices may vary.

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