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General DNA Sequencing Services from Polymorphic DNA Technologies, Inc.

ProductsGeneral DNA Sequencing Services from Polymorphic DNA Technologies, Inc.
Company Polymorphic DNA Technologies, Inc.
Item General DNA Sequencing Services
Price Inquire
Description Polymorphic DNA Technologies also offers a DNA sequencing service. We can sequence a variety of templates including plasmids, PCR products, BACs, etc. Our DNA sequencing service starts with customer-provided templates such as purified plasmids or purified PCR products. We use high throughput liquid handling robotics optimized for automated reaction setup and dye terminator removal. Polymorphic DNA Technologies employs automated high throughput capillary electrophoresis DNA sequencing instruments. Read lengths vary depending on the template provided. Results can be provided electronically by email, secure download from our website or CD.

Applications
Since Polymorphics core technology is Sanger Sequencing, our lab is ideally equipped to provide services for related applications such as SNP discovery, haplotype determination, or closely linked SNP scoring. Our service is also ideal for any short read DNA sequencing application such as bacterial or viral typing (research use only), phage display, confirmation sequencing etc. Our service can also accommodate primer walking projects. For confirmation sequencing, if a reference sequence is provided, we will supply a contig alignment with any variations clearly noted.

Preparation and Shipping
Please complete the DNA Sequencing Order Form and send it to info@polymorphicdna.com. Plasmid DNA or PCR products should be purified by a commercially available gel or column filtration method. Templates should be provided in a sequencing reaction compatible buffer. Please submit templates resuspended in deionized water. 10 mM Tris is also acceptable, but please do not provide samples in TE. Plasmid DNA should be submitted at a concentration of 0.2 μg per μL. Please provide 2 μg of plasmid DNA per sequencing reaction requested. For PCR products, please provide 15 μL of product at a concentration of .02 μg/μL to .05 μg/μL. Please also send a photo of the PCR products run out on an agarose gel.

Primers should be provided at 5 μM with at least 10 μL volume.

Info Polymorphic DNA Technologies, Inc.
1125 Atlantic Av., Ste. 102
Alameda, CA 94501
Customer Service: (888) 362-0888
Fax Number: (510) 523-7628
Web Site: http://www.polymorphicdna.com
NOTE: Price information is approximate list price and actual prices may vary.

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