|Products||GeneRacer 3' Nested Primer from Invitrogen|
|Item||GeneRacer 3' Nested Primer|
|Description||GeneRacer is an advanced RACE (rapid amplification of cDNA ends) technique that improves the efficiency of amplifying full-length 5' and 3' cDNA ends. The GeneRacer Kit is available with SuperScript II Reverse Transcriptase (RT) for improved amplification of the full-length 5 end from long and complex mRNA. The RNase H portion of SuperScript II RT has been inactivated to avoid cleaving mRNA during cDNA synthesis. This increases the size and yield of cDNA. SuperScript II RT is more thermostable than wild-type RTS. This enables reverse transcription at higher temperatures, relaxing secondary structure of complex templates, and allowing cDNA synthesis to go to completion.|
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