|Products||GeneChoice TEMPase II DNA Polymerase from GeneChoice, Inc.|
|Item||GeneChoice TEMPase II DNA Polymerase|
|Description|| • Heat activated|
• Eliminates nonspecific products due to inactivity at room temperature
• Excellent specificity allows extension through DNA regions which contain repeats and secondary structures
• Leaves an 'A' overhang
TEMPase II is a chemically modified form of GeneChoice DNA Polymerase that remains inactive at room temperature and works optimally in a distinctive buffer system.
TEMPase II is useful for performing automated hot start amplification due to its thermal activation at a temperature well above optimal annealing. The enzyme remains inactive until the time, temperature, and pH are optimal. During hot start, primers bind only to their specific target, and polymerase activity is directed exclusively to that target. As a result, only the region of interest is amplified, which increases sensitivity and yield while reducing non-specific background amplification.
Once the reaction reaches optimal annealing temperatures after a 15 minute activation step at 95C, the enzyme stays activated in the reaction.
Since the enzyme is chemically modified for hot start (no antibody), there is no material from animal sources, i.e., there is no possibility for biological contamination.
10X TEMPase II Reaction Buffer - Combination of (NH4)2S04 and KCl, 15 mM MgCl2, 1% Tween 20.
25 mM MgCl2 Solution - Supplied.
|Info|| GeneChoice, Inc.|
7307 Governors Way
Frederick, MD 21703 USA
Fax Number: 301-620-0162
Web Site: http://www.genechoiceinc.com