Navigation Links
GeneAmp PCR Carry-over Prevention Kit from Applied Biosystems

ProductsGeneAmp PCR Carry-over Prevention Kit from Applied Biosystems
Company Applied Biosystems
Item GeneAmp PCR Carry-over Prevention Kit
Price $150.00
Description Enzymatic method stops PCR carry-over contamination, which prevents false positive results Designed to degrade PCR products from previous PCR amplifications without degrading native nucleic acid templates, which improves amplification Optimized for use with GeneAmp PCR core reagents and GeneAmp instrument systems

The GeneAmp PCR Carry-over Prevention Kit provides reagents for a simple yet powerful method of ensuring that PCR products cannot be reamplified in subsequent PCR amplifications, thereby preventing false positive results. This kit uses an enzymatic reaction analogous to the restriction-modification and excision-repair systems of cells to specifically degrade PCR products from previous PCR amplifications in which dUTP has been incorporated, without degrading native nucleic acid templates.

The method used to render PCR products susceptible to degradation involves substituting dUTP for dTTP in the PCR mixture, and pretreating all subsequent PCR mixtures with the enzyme, AmpErase Uracil N-glycosylase (UNG) prior to PCR amplification.1 Products from PCR amplification contain uracil, and are readily distinguishable from native thymidine-containing DNA templates. Products from previous PCR amplifications are eliminated by excising uracil residues using UNG, and degrading the resulting abasic polynucleotide with heat. Although UNG is active on single- and double-stranded dU-containing DNA,2 ribouracil residues in RNA and dUTP itself are not substrates for UNG.3

AmpliTaq and AmpliTaq Gold DNA polymerase, and the other components of the PCR reaction mixture remain intact during UNG treatment, providing PCR amplification free of PCR product carry-over. The dU-containing PCR product behaves like dT-containing DNA in blotting, cloning, sequencing, and most other post-PCR analyses.4

1. Longo, M.C., et al. 1990. Gene 93:125-128
2. Varshney, U., et al. 1988. J. Biol. Chem.263:7,776-7,784.
3. Lindahl, T., et al. 1977. J. Biol. Chem.252:3,286-3,294.
4. Erlich, H.A. et al. 1991. Science 252: 1,643-1,651. For Research Use Only. Not for use in diagnostic procedures.

Info Applied BiosystemsApplied Biosystems
850 Lincoln Centre Drive
Foster City, CA 94404

Call Applied Biosystems to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-650-425-3266
Customer Service: 650-638-5800
Fax Number: 650-638-5884
Web Site: http://home.appliedbiosystems.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. GeneAmp AccuRT RNA PCR Kit with Controls from Applied Biosystems
2. GeneAmp PCR System 2700 from Applied Biosystems
3. Gold-plated 96-Well GeneAmp PCR System 9700 from Applied Biosystems
4. GeneAmp High Fidelity PCR from Applied Biosystems
5. GeneAmplimer HIV-1 Control Reagents from Applied Biosystems
6. ProteinPilot Software from Applied Biosystems
7. Applied Biosystems 2720 Thermal Cycler from Applied Biosystems
8. 4000 Q TRAP LC/MS/MS System from Applied Biosystems
9. Genome Sequencer 20 System from Roche Applied Science
10. RTS AviTag E. coli Biotinylation Kit, Plasmid; Cloning vector and in vitro protein mono-biotinylation system for use with RTS 100 and RTS 500 E. coli HY Kits from Roche Applied Science
11. RTS AviTag E. coli Biotinylation Kit, Linear Template; Linear template generation and in vitro protein mono-biotinylation system for use with RTS 100 expression reactions from Roche Applied Science
...
... Dr. P Set from different Cynomolgus ... More organs maybe available upon request. ... items from the whole set, and ... price. All Dr. P Set ...
...
Immunogen: Recombinant full length protein, corresponding to amino acids 1-435 of Human AP2M1. Storage: -20 C, Avoid Freeze/Thaw Cycles...
Biology Products:
(Date:10/1/2014)... TURKU , Finnland, October 1, 2014 ... der Durchbruch im Bereich der Biowissenschaften   ... Jahren ein Kommen und Gehen zahlreicher Detektionstechnologien. ... sich zum goldenen Standard. Die Photonen-Upconversion ist ... den letzten Jahren mit Begeisterung aufgenommen wurde. ...
(Date:9/30/2014)... effects of moorland burning is published today in the ... aim of relieving tensions on both sides of the ... of Moorland Burning on the Ecohydrology of River basins) ... is practised predominantly to support red grouse populations for ... peat chemistry and physical properties, river water chemistry and ...
(Date:9/30/2014)... seedless watermelon, salmon, and strawberries all have in common? ... sets of chromosomes and are diploid, these organisms are ... of chromosomesseedless watermelon and salmon have 3 and 4 ... While this might seem surprising, in fact most plant ... discovered over a century ago, but only recently, with ...
Breaking Biology News(10 mins):Innovative Verwendungslösung für die Photonen-Upconversion 2Innovative Verwendungslösung für die Photonen-Upconversion 3Grouse moor burning causes widespread environmental changes 2Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 2Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 3Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 4
... that has wiped out more than 100 species, scientists at ... species in Panama: Pristimantis educatoris , from Omar Torrijos ... near the Colombian border. , In 1989 researchers realized that ... cause: a fungal disease called chytridiomycosis. In 2004 Karen Lips, ...
... than two million miles of the nation,s ... nearing the end of its useful life, ... not attract much attention because of this ... professor of civil and environmental engineering (CEE). ...
... at Oregon State University have made an important fundamental advance ... not only can walk and run effectively, but use little ... with robotic mechanisms, studies are moving closer to robots that ... limbs for humans that work much better, or even help ...
Cached Biology News:2 new frog species discovered in Panama's fungal war zone 2Efforts underway at Virginia Tech to address deterioration of nation's water pipes 2Advances made in walking, running robots 2Advances made in walking, running robots 3
(Date:10/1/2014)... Francisco, California (PRWEB) October 01, 2014 ... from the National Science Foundation to expand their bioCAD/CAM ... to build and modify DNA. The Small Business Innovation ... commercialize the “j5” technology under exclusive license from the ... Joint BioEnergy Institute , an LBNL laboratory that ...
(Date:10/1/2014)... , October 1, 2014 ... software invited to engage around the theme, ,Commercial Opportunities ...   Elsevier , a ... and services, will host a gathering of some of ... and trends impacting healthcare today and in the future. ...
(Date:9/30/2014)... news release is available in German . ... the Technische Universitt Mnchen (TUM) have demonstrated a new ... experiments show that future computer chips could be based ... As the main enabling technology of the semiconductor industry ... limits, the TUM researchers and collaborators at the University ...
(Date:9/30/2014)... , Sept. 30, 2014 Report Details ... with highest potential revenues Do you want to ... gives you revenue predictions for those biological drugs ... therapies you find financial data, R&D trends, opportunities ... get sales forecasts to 2024 at overall world ...
Breaking Biology Technology:TeselaGen Receives $750k NSF SBIR Phase II Award 2TeselaGen Receives $750k NSF SBIR Phase II Award 3Elsevier Healthcare and Pharmaceutical Economics Conference 2014 To Convene Industry Leaders to Discuss Top Healthcare Business Issues 2Elsevier Healthcare and Pharmaceutical Economics Conference 2014 To Convene Industry Leaders to Discuss Top Healthcare Business Issues 3A new dimension for integrated circuits: 3-D nanomagnetic logic 2A new dimension for integrated circuits: 3-D nanomagnetic logic 3Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 2Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 3Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 4Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 5