Navigation Links
GeneAmp PCR Carry-over Prevention Kit from Applied Biosystems

ProductsGeneAmp PCR Carry-over Prevention Kit from Applied Biosystems
Company Applied Biosystems
Item GeneAmp PCR Carry-over Prevention Kit
Price $150.00
Description Enzymatic method stops PCR carry-over contamination, which prevents false positive results Designed to degrade PCR products from previous PCR amplifications without degrading native nucleic acid templates, which improves amplification Optimized for use with GeneAmp PCR core reagents and GeneAmp instrument systems

The GeneAmp PCR Carry-over Prevention Kit provides reagents for a simple yet powerful method of ensuring that PCR products cannot be reamplified in subsequent PCR amplifications, thereby preventing false positive results. This kit uses an enzymatic reaction analogous to the restriction-modification and excision-repair systems of cells to specifically degrade PCR products from previous PCR amplifications in which dUTP has been incorporated, without degrading native nucleic acid templates.

The method used to render PCR products susceptible to degradation involves substituting dUTP for dTTP in the PCR mixture, and pretreating all subsequent PCR mixtures with the enzyme, AmpErase Uracil N-glycosylase (UNG) prior to PCR amplification.1 Products from PCR amplification contain uracil, and are readily distinguishable from native thymidine-containing DNA templates. Products from previous PCR amplifications are eliminated by excising uracil residues using UNG, and degrading the resulting abasic polynucleotide with heat. Although UNG is active on single- and double-stranded dU-containing DNA,2 ribouracil residues in RNA and dUTP itself are not substrates for UNG.3

AmpliTaq and AmpliTaq Gold DNA polymerase, and the other components of the PCR reaction mixture remain intact during UNG treatment, providing PCR amplification free of PCR product carry-over. The dU-containing PCR product behaves like dT-containing DNA in blotting, cloning, sequencing, and most other post-PCR analyses.4

1. Longo, M.C., et al. 1990. Gene 93:125-128
2. Varshney, U., et al. 1988. J. Biol. Chem.263:7,776-7,784.
3. Lindahl, T., et al. 1977. J. Biol. Chem.252:3,286-3,294.
4. Erlich, H.A. et al. 1991. Science 252: 1,643-1,651. For Research Use Only. Not for use in diagnostic procedures.

Info Applied BiosystemsApplied Biosystems
850 Lincoln Centre Drive
Foster City, CA 94404

Call Applied Biosystems to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-650-425-3266
Customer Service: 650-638-5800
Fax Number: 650-638-5884
Web Site: http://home.appliedbiosystems.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. GeneAmp AccuRT RNA PCR Kit with Controls from Applied Biosystems
2. GeneAmp PCR System 2700 from Applied Biosystems
3. Gold-plated 96-Well GeneAmp PCR System 9700 from Applied Biosystems
4. GeneAmp High Fidelity PCR from Applied Biosystems
5. GeneAmplimer HIV-1 Control Reagents from Applied Biosystems
6. ProteinPilot Software from Applied Biosystems
7. Applied Biosystems 2720 Thermal Cycler from Applied Biosystems
8. 4000 Q TRAP LC/MS/MS System from Applied Biosystems
9. Genome Sequencer 20 System from Roche Applied Science
10. RTS AviTag E. coli Biotinylation Kit, Plasmid; Cloning vector and in vitro protein mono-biotinylation system for use with RTS 100 and RTS 500 E. coli HY Kits from Roche Applied Science
11. RTS AviTag E. coli Biotinylation Kit, Linear Template; Linear template generation and in vitro protein mono-biotinylation system for use with RTS 100 expression reactions from Roche Applied Science
... iLink makes it easy to supply microplates, ... Developed with operator convenience in mind, iLink ... ease of use. The iLink software is ... assays or sub-assays, that can be copied ...
... integrated digital microscope and camera system that ... optical adjustment or software installation. Built on ... combines simplicity and advanced technology for users ... viewing of a macro image and a ...
... DASGIP Overhead Drive for individual and ... for flexible agitation of DASGIP vessels, featuring ... impellers. ,Up to 16 overhead drives ... DASGIP Cultivation Systems fedbatch-pro® and cellferm-pro® for ...
This kit is designed to select cells labeled with biotinylated antibodies. Kit includes reagents for labeling 3 x 10 9 cells and EasySep magnet....
Biology Products:
(Date:9/15/2014)... diseases requires that the proteins in our cells cooperate ... secret how tens of thousands of different proteins find ... up the human body, brain and nervous system. A ... Chemistry has busted down the door and provided a ... dance floor. , Professor emeritus Jens Jrgen Led of ...
(Date:9/15/2014)... life it,s not what comes to mind when you ... of Doncaster,s 310-million-year-old past, including a fossilised shark egg case, ... of the fossilised plants and creatures may even be new ... horseshoe crabs and some previously unrecorded seed pods are amongst ... formed within the coal and shale deposits in what is ...
(Date:9/15/2014)... , , , , , ... , , , , ... disorders, each with its own set of symptoms. The finding could be a first... , , ... , , , , , ... , , , , , , , New ...
Breaking Biology News(10 mins):Protein courtship revealed through chemist's lens 2Protein courtship revealed through chemist's lens 3'Jaws' lived in Doncaster 2Schizophrenia not a single disease but multiple genetically distinct disorders 2Schizophrenia not a single disease but multiple genetically distinct disorders 3Schizophrenia not a single disease but multiple genetically distinct disorders 4Schizophrenia not a single disease but multiple genetically distinct disorders 5
... COLUMBIA, Mo. Pairing a new approach to prepare ... to genotype a cow, an MU animal scientist, along ... accurate and widespread "family tree" for cows and other ... This genetic information could allow scientists to understand the ...
... Old Orchard Beach, Maine, a doctoral student in ... fellowship from the Environmental Protection Agency,s (EPA) National ... the reproductive success of American toads. Bergeron,s ... Results (STAR) program, ( http://www.epa.gov/ncer/fellow/ ), will support ...
... Tropical Research Institute in Panama has dramatically improved the ... spread of chytridiomycosisone of the deadliest frog diseases on ... dendrobatidis , this disease is probably responsible for the ... During the past decade, the epidemic swept from the ...
Cached Biology News:MU research team establishes family tree for cattle, other ruminants 2EPA fellow studies effect of mercury in toads 2Catching a killer one spore at a time 2
(Date:9/15/2014)... , Sept. 15, 2014 Research and ... Exome Sequencing Market by Product, by Services, by Technology, by ... offering. Whole Exome Sequencing (WES) is next-generation ... genome, i.e. the exon. The exome, which is the total ... the total genome and is actually converted into proteins after ...
(Date:9/15/2014)... 15. September 2014 Die zweite jährliche ... vom 12. bis 18. Oktober stattfinden. Die IPAW ... Therapeutics Association (PPTA) und ihrer Mitgliedsunternehmen ... Das globale Bewusstsein für die Gewinnung von Quellplasma ... beim Retten und Verbessern von Leben zu würdigen ...
(Date:9/15/2014)... Md. , Sept. 15, 2014  The second ... be celebrated October 12-18. A joint initiative of the ... member companies , IPAW is designed to: ... , Recognize the contributions of plasma donors in ... lifesaving plasma protein therapies and rare diseases ...
(Date:9/14/2014)... regulate protein functions in a wide variety of ways, ... a protein can take on another form and perform ... that fold wrongly can cause serious diseases such as ... also have a tendency to "infect" other molecules of ... fibrils or plaques. These amyloids can damage cells and ...
Breaking Biology Technology:Global Whole Exome Sequencing (Systems, Kits, Library Preparation, Target Enrichment) Market by Product, by Services, by Technology, by Application - Forecast to 2018 2Global Whole Exome Sequencing (Systems, Kits, Library Preparation, Target Enrichment) Market by Product, by Services, by Technology, by Application - Forecast to 2018 3International Plasma Awareness Week feiert Spender und rückt seltene Krankheiten in den Mittelpunkt 2International Plasma Awareness Week feiert Spender und rückt seltene Krankheiten in den Mittelpunkt 3International Plasma Awareness Week To Celebrate Donors And Spotlight Rare Diseases 2International Plasma Awareness Week To Celebrate Donors And Spotlight Rare Diseases 3Measuring modified protein structures 2Measuring modified protein structures 3