Navigation Links
GeneAmp PCR Carry-over Prevention Kit from Applied Biosystems

ProductsGeneAmp PCR Carry-over Prevention Kit from Applied Biosystems
Company Applied Biosystems
Item GeneAmp PCR Carry-over Prevention Kit
Price $150.00
Description Enzymatic method stops PCR carry-over contamination, which prevents false positive results Designed to degrade PCR products from previous PCR amplifications without degrading native nucleic acid templates, which improves amplification Optimized for use with GeneAmp PCR core reagents and GeneAmp instrument systems

The GeneAmp PCR Carry-over Prevention Kit provides reagents for a simple yet powerful method of ensuring that PCR products cannot be reamplified in subsequent PCR amplifications, thereby preventing false positive results. This kit uses an enzymatic reaction analogous to the restriction-modification and excision-repair systems of cells to specifically degrade PCR products from previous PCR amplifications in which dUTP has been incorporated, without degrading native nucleic acid templates.

The method used to render PCR products susceptible to degradation involves substituting dUTP for dTTP in the PCR mixture, and pretreating all subsequent PCR mixtures with the enzyme, AmpErase Uracil N-glycosylase (UNG) prior to PCR amplification.1 Products from PCR amplification contain uracil, and are readily distinguishable from native thymidine-containing DNA templates. Products from previous PCR amplifications are eliminated by excising uracil residues using UNG, and degrading the resulting abasic polynucleotide with heat. Although UNG is active on single- and double-stranded dU-containing DNA,2 ribouracil residues in RNA and dUTP itself are not substrates for UNG.3

AmpliTaq and AmpliTaq Gold DNA polymerase, and the other components of the PCR reaction mixture remain intact during UNG treatment, providing PCR amplification free of PCR product carry-over. The dU-containing PCR product behaves like dT-containing DNA in blotting, cloning, sequencing, and most other post-PCR analyses.4

1. Longo, M.C., et al. 1990. Gene 93:125-128
2. Varshney, U., et al. 1988. J. Biol. Chem.263:7,776-7,784.
3. Lindahl, T., et al. 1977. J. Biol. Chem.252:3,286-3,294.
4. Erlich, H.A. et al. 1991. Science 252: 1,643-1,651. For Research Use Only. Not for use in diagnostic procedures.

Info Applied BiosystemsApplied Biosystems
850 Lincoln Centre Drive
Foster City, CA 94404

Call Applied Biosystems to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-650-425-3266
Customer Service: 650-638-5800
Fax Number: 650-638-5884
Web Site: http://home.appliedbiosystems.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. GeneAmp AccuRT RNA PCR Kit with Controls from Applied Biosystems
2. GeneAmp PCR System 2700 from Applied Biosystems
3. Gold-plated 96-Well GeneAmp PCR System 9700 from Applied Biosystems
4. GeneAmp High Fidelity PCR from Applied Biosystems
5. GeneAmplimer HIV-1 Control Reagents from Applied Biosystems
6. ProteinPilot Software from Applied Biosystems
7. Applied Biosystems 2720 Thermal Cycler from Applied Biosystems
8. 4000 Q TRAP LC/MS/MS System from Applied Biosystems
9. Genome Sequencer 20 System from Roche Applied Science
10. RTS AviTag E. coli Biotinylation Kit, Plasmid; Cloning vector and in vitro protein mono-biotinylation system for use with RTS 100 and RTS 500 E. coli HY Kits from Roche Applied Science
11. RTS AviTag E. coli Biotinylation Kit, Linear Template; Linear template generation and in vitro protein mono-biotinylation system for use with RTS 100 expression reactions from Roche Applied Science
... Polymerase is ideal for high-fidelity amplification ... such as cloning and mutagenesis. ... proprietary enzyme preparation containing recombinant DNA ... (3'Cut Site5' exonuclease) activity. Platinum ...
... PLAU N-term Antibody This antibody is generated ... synthetic peptide selected within aa 50~150 of ... in PBS with 0.09% (W/V) sodium azide. ... G column and eluted out with both ...
...
Human interferon-beta (IFN-beta). Recognizes amino acids 30-47 of IFN-beta. No cross-reactivity with Human IFN-alpha or IFN-gamma....
Biology Products:
(Date:12/10/2014)... RALEIGH, N.C. , Dec. 9, 2014  Valencell, ... announced it is seeing a staggering demand from its ... demand, the Company revealed, is not solely coming from ... industries as well. "A wearable is only ... a result, accuracy is the ultimate driver in long-term ...
(Date:12/10/2014)... CIE San Diego has launched an innovative ... tissue that enhances care coordination among social service and ... information; earned a second $1 million grant from the ... aging in community and; will be recognized as a ... 4-6p. CIE San Diego today announced ...
(Date:12/10/2014)... , December 9, 2014 ... ITN,s BCARD Platform   ... enterprise meeting scheduling solutions for business-to-business (B2B) events, ... world leader in mobile near-field communication (NFC), Bluetooth ...      (Logo: http://photos.prnewswire.com/prnh/20140612/691055) , Jifflenow ...
Breaking Biology News(10 mins):Wearable Technology Products Demand Highly Accurate Biometric Technology 2Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 2Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 3Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 4Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 2Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 3
... coin or a watch can be dangerous for people ... is allergic to the metal. "This raises the question ... Professor Dr. Markus Rettenmayr of the Friedrich Schiller University ... for cardiovascular implants in minimal invasive surgery. Once implanted, ...
... CHICAGO Solving crucial environmental issues such as ... interests, uncertainty and risk, and this is best ... Arizona State University Barrett Honors College ... which scientists, stakeholders and policy makers can communicate ...
... a range of climate change mitigation scenarios are discussed, ... of sulfate particles into the atmosphere to reflect sunlight ... a severe threat if not maintained indefinitely and supported ... The new study, published today, 18 February, in IOP ...
Cached Biology News:Metal in the heart is non-hazardous to health 2Environmental issues examined through cohesive efforts 2Researchers warn against abrupt stop to geoengineering method 2
(Date:12/24/2014)... Dec. 24, 2014   BioLife Solutions , Inc. ... and marketer of proprietary clinical grade hypothermic storage ... precision thermal shipping products for cells and tissues  ... hold its 2015 Annual Meeting of Stockholders on May ... expected date for the Annual Meeting represents a change ...
(Date:12/24/2014)... MD (PRWEB) December 23, 2014 ... into law H.R. 83, the Omnibus and Continuing ... first time, made hydrocephalus a condition eligible to ... Programs (CDMRP) administered by the Department of Defense ... with its allies on Capitol Hill, is celebrating ...
(Date:12/24/2014)... December 23, 2014 Pipette.com announces their ... Twitter followers can submit their #HolidayInTheLab pictures for a ... lab mates. , For those struggling to think of ... Pipette.com Holiday in the Lab Contest provides the ideal ... followers to send pictures of their chemistree, holiday ...
(Date:12/24/2014)... SARASOTA, Fla. , Dec. 23, 2014  Rock ... today that it has filed a Clinical Trial Application ... Medicines Healthcare products Regulatory Agency (MHRA) seeking regulatory approval ... its lead molecule, Anatabine Citrate. Contingent on ... to conduct a Phase I trial to assess the ...
Breaking Biology Technology:BioLife Solutions Sets Date for Annual Meeting of Stockholders 2Rare Brain Condition Now Eligible for Department of Defense Research Dollars 2Major Pipette Distributor Pipette.com Announces their Holiday in the Lab Contest 2Rock Creek Pharmaceuticals Files Clinical Trial Application in the UK for Clinical Trials of Anatabine Citrate Formulations 2Rock Creek Pharmaceuticals Files Clinical Trial Application in the UK for Clinical Trials of Anatabine Citrate Formulations 3