Navigation Links
GenElute Agarose Spin Columns from Sigma-Aldrich

ProductsGenElute Agarose Spin Columns from Sigma-Aldrich
Company Sigma-Aldrich
Item GenElute Agarose Spin Columns
Price 
Description GenElute™ Agarose Spin Columns are designed for the rapid purification of linear 100 bp to 10 kb DNA from agarose gels. The DNA band is excised from an agarose gel and loaded into the spin column. Embedded within the base of the column are a series of membranes and filters that hold agarose and impurities back while allowing DNA to selectively pass through the column into a collection tube during a 10 minute centrifugation. GenElute Agarose Spin Columns eliminate the need for silica-based resins, DEAE, or toxic organic solvents such as phenol and chloroform. There is no melting, electroelution, or enzymatic digestion of the agarose gel.

Typical recovery is 40 to 45% for 100 bp to 10 kb DNA fragments. Recovery decreases with increasing fragment size. Optimal recovery can be obtained with gel slices weighing less than 100 mg. A 40 mg gel slice from a 1.0% gel will yield an eluate volume of approximately 40 l. A 90 mg gel slice from a 1.0% gel will yield an eluate volume of approximately 65 l. The maximum gel slice weight is approximately 200 mg. The purified DNA can be used in most downstream applications such as ligation, PCR, restriction endonuclease digestion, labeling and hybridization.
Info Sigma-AldrichSigma-Aldrich
Sigma-Aldrich Corp.
St. Louis, MO, USA
Customer Service: 800-325-3010
Tech Support: 800-325-5832
Fax Number: 314-771-5757
Web Site: http://www.sigmaaldrich.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. GenElute Minus EtBr Spin Column from Sigma-Aldrich
2. GenElute Gel Extraction Kit from Sigma-Aldrich
3. S-Protein Agarose from Novagen
4. S-Protein Agarose from Novagen
5. S-Protein Agarose from Novagen
6. UltraPure Agarose and SYBR Safe DNA Gel Stain Combo from Invitrogen
7. UltraPure Agarose and SYBR Safe DNA Gel Stain combo from Invitrogen
8. GELase LMP Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
9. GELase LMP Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
10. GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
11. GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
... Edwards (1996) • Provides key ... immunoassays, including RIA, ELISA and ... the photoluminescent and chemiluminescent labels ... also given on assay design ...
EoProbe Eosinophil Staining Kit, 1 Kit...
... and M.W. Turner (1997). ... step-by-step methods for the ... molecules, the use of ... analytical probes and as ...
B.D. Hames and D.M. Glover (1996) • This issue covers T-Cell antigen receptor genes; T-Lymphocyte signal transduction; B-Cell activation antibody engineering and the complement system and other ...
Biology Products:
(Date:5/16/2017)...   Bridge Patient Portal , an enterprise ... EMR Systems , an electronic medical record solutions ... established a partnership to build an interface between ... Centricity™ products, including Centricity Practice Solution (CPS), Centricity ... new integrations will allow healthcare delivery networks using ...
(Date:4/17/2017)... NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or the "Company"), ... Report on Form 10-K on Thursday April 13, 2017 with the ... The ... section of the Company,s website at http://www.nxt-id.com  under "SEC Filings," ... 2016 Year Highlights: Acquisition of ...
(Date:4/11/2017)... N.Y. , April 11, 2017 ... fingerprints, but researchers at the New York University ... College of Engineering have found that partial similarities ... security systems used in mobile phones and other ... thought. The vulnerability lies in the ...
Breaking Biology News(10 mins):
(Date:7/17/2017)... (PRWEB) , ... July 17, ... ... of technology-enabled clinical trial solutions, today announced safety software company AB Cube ... the eHealth Solutions business segment to advance technology innovation across life sciences ...
(Date:7/17/2017)... , ... July 17, 2017 , ... ... characterized by a wide range of overlapping clinical features. The advancement of targeted ... the field of NDD research and testing. , However, designing a custom ...
(Date:7/17/2017)... ... July 17, 2017 , ... Co-Diagnostics, Inc. ... and intends both to manufacture and sell reagents used for diagnostic tests, has ... , Headquartered in Sandy, Utah, Co-Diagnostics’ intellectual property and technologies are protected by ...
(Date:7/16/2017)... (PRWEB) , ... July 16, 2017 , ... ... equipment and analytical instruments announced the launch of its new line of Rocking ... five rocking and waving shaker models (both analog and digital) for laboratory applications ...
Breaking Biology Technology: