Navigation Links
GPS -M Mutagenesis System from New England Biolabs

ProductsGPS -M Mutagenesis System from New England Biolabs
Company New England Biolabs
Item GPS -M Mutagenesis System
Price $300.00
Description The GPS-M Mutagenesis System enables you to insert a transposable element of your own design into your target DNA by in vitro transposition. It uses TnsABC* Transposase to insert a Tn7-based transposon randomly into the DNA target. Target DNA may be a plasmid, cosmid, BAC or purified chromosomal DNA, depending on your interests (6-8). If the insertion site is within a translated gene segment, this will normally result in a null (loss of function) mutation. There is minimal site preference for insertion, so disruption of any open reading frame should be possible. Due to target immunity, only one insertion occurs per DNA molecule. Target immunity acts in vivo over a distance of ~190 kb.Therefore, the in vitro reaction produces a population of target DNA molecules each containing the transposable element at a different position. To facilitate adaptation of the transposable element to your particular mutagenesis application, the transposon donor (pGPS3) can be customized by adding to or replacing the kanamycin resistance marker in the Transprimer. For ease of manipulation by the user, the donor plasmid can be grown easily in standard laboratory E. coli strains; the vector backbone carries Ampr and a pUC origin of replication. To destroy unreacted donor molecules and avoid undesirable reaction products, the donor can be destroyed by digestion with the rare-cutting enzyme PI-Sce I (VDE). For applications in which the mutagenized DNA will be transformed into naturally-competent organisms (which take up single DNA strands), a protocol is supplied for filling-in and ligating the gaps left by the insertion transposition reaction. Notes: The Kanr marker on the Transprimer is not designed to express in Gram positive organisms or eukaryotic cells. Transposon ends have stop codons in all frames, so translational fusions are not practical.
Info New England BiolabsNew England Biolabs
32 Tozer Rd.
Beverly, MA 01915-5599

Customer Service: 800-632-5227
Tech Support: 800-632-7799
Fax Number: 978-921-1350
Web Site: http://www.neb.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Site-directed Mutagenesis from Exon BioSystems
2. Site-directed DNA Mutagenesis Service from Yorkshire Bioscience Ltd
3. AMAP Multi Site-directed Mutagenesis Kit from MBL International
4. GeneTailor Site-Directed Mutagenesis System from Invitrogen
5. R.A.P.I.D. System from Idaho Technology Inc.
6. CEQ 8800 Genetic Analysis System from Beckman Coulter
7. ProteomeLab PA 800 Protein Characterization System from Beckman Coulter
8. ProteomeLab PF 2D Protein Fractionation System from Beckman Coulter
9. pET GST Fusion System 42 plus Competent Cells from Novagen
10. pET GST Fusion System 42 from Novagen
11. pET GST Fusion System 41 plus Competent Cells from Novagen
(Date:8/11/2015)... August 11, 2015 Today, ZUK announced its ... as well as expected revenues in 2015 that relate to sales ... revenue guidance of approximately 2,200 MSEK for 2015. Jörgen ... prominent smartphone manufacturer in China ... selected FPC1 155 for Z1 ...
(Date:8/6/2015)... Germany , August 6, 2015 ... (SMI) shows the world,s first Eye Tracking ... Epson,s Moverio BT-200 see-through head mounted display and on ... unprecedented quality and efficiency is brought to personalized visualization ... the first time, professionals and researchers can integrate gaze, ...
(Date:8/5/2015)... , Aug. 5, 2015 The biosensors market ... in applications, penetration into newer sectors, and development of ... global biosensors space has seen the entry of multiple ... so far. (Photo - http://photos.prnewswire.com/prnh/20150805/255570 ) ... Analysis of the Global Biosensors Market ( ...
Breaking Biology News(10 mins):FPC's Touch Fingerprint Sensor FPC1155 in ZUK's Smartphone Z1 2SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 2SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 3Innovative Biosensors Incite Use in Non-Traditional Applications 2Innovative Biosensors Incite Use in Non-Traditional Applications 3Innovative Biosensors Incite Use in Non-Traditional Applications 4
... is available in Spanish . , Adolescents of ... and regular physical activity present increased levels of insulin and ... appetite control and energy expenditure. This was the conclusion drawn ... of the University of Granada . Further, after thorough ...
... of diabetes risk than does BMI. This is the ... the German Institute for Nutritional Research in Potsdam-Rehbrcke, in ... (Dtsch Arztebl Int 2010; 107[26]: 470-6). Current guidelines ... overweight should be based on the determination of the ...
... Minimally invasive surgery is increasingly common and effective for ... which use slender, handheld tools inserted into the body ... the most important factor determining the success of the ... Polytechnic Institute has won a $2.3 million federal grant ...
Cached Biology News:Children in good shape have better appetite control and energy expenditure 2Designing touch-sensitive virtual reality tools to train and test tomorrow's surgeons 2Designing touch-sensitive virtual reality tools to train and test tomorrow's surgeons 3
(Date:8/31/2015)... DIEGO , Aug. 31, 2015 MabVax ... development company announces that the Company,s lead antibody, HuMab ... of five separate presentations during the upcoming World Molecular ... September 2-5, 2015. Researchers from the Department ... will present results on the use of MabVax,s lead ...
(Date:8/31/2015)... people with Down syndrome, news from Elixirgen, LLC ... in the Science + Technology Park at Johns Hopkins, ... for people with Down syndrome and other chromosome disorders in ... Edwards syndrome aneuploidies in human cell cultures, " published ... to develop this technology into a treatment for the ...
(Date:8/31/2015)... 31, 2015 FLX Bio, Inc., a biopharmaceutical ... cancer immunotherapies, announced today that it has appointed ... and William Ho , M.D., Ph.D., as Chief ... management team led by veterans of Flexus Biosciences, Inc., ... Scientific Officer; Jay Powers , Ph.D., Vice President, ...
(Date:8/31/2015)... ... August 31, 2015 , ... There’s a new treatment option for excessive ... commercially available new FDA approved system in the last 14 years, and the only ... Amenorrhea (zero bleeding) rate of 66%, the high Patient Satisfaction rate of 98%, and ...
Breaking Biology Technology:MabVax Therapeutics HuMab 5B1 Antibody Is Highlighted at Upcoming World Molecular Imaging Congress in Five Separate Presentations 2MabVax Therapeutics HuMab 5B1 Antibody Is Highlighted at Upcoming World Molecular Imaging Congress in Five Separate Presentations 3MabVax Therapeutics HuMab 5B1 Antibody Is Highlighted at Upcoming World Molecular Imaging Congress in Five Separate Presentations 4New Down syndrome therapy discovered 2FLX Bio Announces Senior Management Hires and Initiation of Phase 1 Trial of FLX925 2FLX Bio Announces Senior Management Hires and Initiation of Phase 1 Trial of FLX925 3FLX Bio Announces Senior Management Hires and Initiation of Phase 1 Trial of FLX925 4U.S. FDA Approves Minerva Endometrial Ablation System 2U.S. FDA Approves Minerva Endometrial Ablation System 3