Navigation Links
GPS -M Mutagenesis System from New England Biolabs

ProductsGPS -M Mutagenesis System from New England Biolabs
Company New England Biolabs
Item GPS -M Mutagenesis System
Price $300.00
Description The GPS-M Mutagenesis System enables you to insert a transposable element of your own design into your target DNA by in vitro transposition. It uses TnsABC* Transposase to insert a Tn7-based transposon randomly into the DNA target. Target DNA may be a plasmid, cosmid, BAC or purified chromosomal DNA, depending on your interests (6-8). If the insertion site is within a translated gene segment, this will normally result in a null (loss of function) mutation. There is minimal site preference for insertion, so disruption of any open reading frame should be possible. Due to target immunity, only one insertion occurs per DNA molecule. Target immunity acts in vivo over a distance of ~190 kb.Therefore, the in vitro reaction produces a population of target DNA molecules each containing the transposable element at a different position. To facilitate adaptation of the transposable element to your particular mutagenesis application, the transposon donor (pGPS3) can be customized by adding to or replacing the kanamycin resistance marker in the Transprimer. For ease of manipulation by the user, the donor plasmid can be grown easily in standard laboratory E. coli strains; the vector backbone carries Ampr and a pUC origin of replication. To destroy unreacted donor molecules and avoid undesirable reaction products, the donor can be destroyed by digestion with the rare-cutting enzyme PI-Sce I (VDE). For applications in which the mutagenized DNA will be transformed into naturally-competent organisms (which take up single DNA strands), a protocol is supplied for filling-in and ligating the gaps left by the insertion transposition reaction. Notes: The Kanr marker on the Transprimer is not designed to express in Gram positive organisms or eukaryotic cells. Transposon ends have stop codons in all frames, so translational fusions are not practical.
Info New England BiolabsNew England Biolabs
32 Tozer Rd.
Beverly, MA 01915-5599

Customer Service: 800-632-5227
Tech Support: 800-632-7799
Fax Number: 978-921-1350
Web Site: http://www.neb.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Site-directed Mutagenesis from Exon BioSystems
2. Site-directed DNA Mutagenesis Service from Yorkshire Bioscience Ltd
3. AMAP Multi Site-directed Mutagenesis Kit from MBL International
4. GeneTailor Site-Directed Mutagenesis System from Invitrogen
5. R.A.P.I.D. System from Idaho Technology Inc.
6. CEQ 8800 Genetic Analysis System from Beckman Coulter
7. ProteomeLab PA 800 Protein Characterization System from Beckman Coulter
8. ProteomeLab PF 2D Protein Fractionation System from Beckman Coulter
9. pET GST Fusion System 42 plus Competent Cells from Novagen
10. pET GST Fusion System 42 from Novagen
11. pET GST Fusion System 41 plus Competent Cells from Novagen
Kinase Buffer can be used to assay protein kinase activity...
Goat polyclonal to SPFH2 ( Abpromise for all tested applications). Antigen: Synthetic peptide: EDEPLETATKEN, corresponding to C terminal amino acids 328-339 of Human SPFH2 Entrez Gene ID: 11...
Anti-Kusabira Orange Monoclonal Antibody Description: 100 g Research Focus: tag Storage: -20C Shipping Temperature: 4C...
alpha/beta-Tubulin Antibody Ship: Hot Store: -20 C...
Biology Products:
(Date:12/17/2014)... NEW YORK , Dec. 15, 2014 /PRNewswire-USNewswire/ ... a Good Clinical Practices (GCP) audit to confirm ... Administration (FDA) regulations. This accomplishment enables HITLAB to ... trials utilizing the highest principles for patient safety ... to improve global healthcare access, quality, and delivery ...
(Date:12/17/2014)... Dec. 16, 2014  Automation is fundamentally transforming ... evident than at international borders. Over the past ... have allowed veteran travelers to self process through ... (APC) Kiosks at an increasing number of airports, ... According to Maxine Most , ...
(Date:12/11/2014)... 09, 2014 Research and Markets ... ( http://www.researchandmarkets.com/research/9ql3kr/biometrics_market ) has announced the addition ... report to their offering. ... the adoption of multimodal biometric systems. Multimodal biometric ... individual for verification and identification purposes. This helps ...
Breaking Biology News(10 mins):HITLAB Completes GCP Audit for FDA Smart Device & App Compliance 2Automated Border Control (ABC) Transforms the Global Travel Experience With More Than 2500 ABC eGates and APC Kiosks Deployed In Airports, Seaports, and Land Borders Worldwide 2Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2
... papers that have been recently published in Geophysical Research ... Earth ( JGR-B ) and Journal of Geophysical ... 1. An earthquake in Japan caused large waves in Norwegian ... in Ohio, 3. The Arctic is especially sensitive to black ...
... Johns Hopkins have uncovered a protein switch that can ... depending on the signals it detects. Its dual role ... network of signals that shapes our brain,s circuitry, the ... the July 31 issue of the Journal of ...
... A strain of bacteria that causes skin and soft tissue ... study to be published in mBio , the online ... who conducted the genetic analysis of strains of Staphylococcus ... to methicillin after they crossed over into humans around forty ...
Cached Biology News:AGU journal highlights -- Aug. 13, 2013 2AGU journal highlights -- Aug. 13, 2013 3AGU journal highlights -- Aug. 13, 2013 4AGU journal highlights -- Aug. 13, 2013 5AGU journal highlights -- Aug. 13, 2013 6AGU journal highlights -- Aug. 13, 2013 7AGU journal highlights -- Aug. 13, 2013 8Newly discovered 'switch' plays dual role in memory formation 2MRSA strain in humans originally came from cattle 2MRSA strain in humans originally came from cattle 3
(Date:12/22/2014)... 2014 GenomeDx Biosciences today announced that a ... a genomic test for prostate cancer, was able to ... by radical prostatectomy without adjuvant therapy. Although rapid metastasis ... using tumor genomics to identify these men who have ... important advance. The study has been published online ...
(Date:12/22/2014)... (PRWEB) December 22, 2014 Fertility Associates ... Donor Egg Bank USA , a network of more ... Through this partnership, patients have fast access to a ... vitro fertilization, IVF . Fertility Associates of Memphis is ... this fertility treatment option. , Dr. William Kutteh ...
(Date:12/19/2014)... , Dec. 19, 2014 Naurex Inc., ... novel drugs for diseases of the central nervous system, ... and chief executive officer, will present at the 33 ... presentation will take place at 3:00 p.m. PST on ... in San Francisco, Calif. ...
(Date:12/19/2014)... DUBLIN , Dec. 19, 2014 Research ... announced the addition of the "Technology Innovations ... their offering. http://photos.prnewswire.com/prnh/20130307/600769 ... Innovations in Smart Fabrics" offers a detailed assessment ... Key Findings: 1. ...
Breaking Biology Technology:Study Published in European Urology Shows that the Decipher Prostate Cancer Classifier is Predictive of Rapid Metastasis in High-Risk Men who have had Prostate Surgery 2Study Published in European Urology Shows that the Decipher Prostate Cancer Classifier is Predictive of Rapid Metastasis in High-Risk Men who have had Prostate Surgery 3Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 3Naurex to Present at 33rd Annual J.P. Morgan Healthcare Conference 2Technology Innovations in Smart Fabrics (Technical Insights) 2