Navigation Links
Flow Cytometry Kit for Apoptosis from Sigma-Aldrich

ProductsFlow Cytometry Kit for Apoptosis from Sigma-Aldrich
Company Sigma-Aldrich
Item Flow Cytometry Kit for Apoptosis
Price 
Description Application: The greater incorporation of Br-dUTP results in a stronger signal by flow cytometry when detected using a fluorescein-labeled anti-BrdU antibody. Propidium iodide/RNase A solution is included in the kit to counterstain the total DNA.
Features and Benefits: Greater incorporation of Br-dUTP, resulting in improved detection than found by using biotin- or digoxigenin-conjugated dUTP or by using fluorochrome (fluorescein or BODIPY)-conjugated deoxynucleotides.
Packaging: The kit is shipped in two parts, both wet ice. Upon receipt, store APO-PART1 at −20C and APO-PART2 at 2-4C
Preparation Principle: Br-dUTP is incorporated more readily into the DNA fragments than deoxyuridine triphosphate labeled with larger dyes such as FITC, biotin or digoxigenin. One of the biological characteristics that defines apoptosis is the degradation of genomic DNA into fragments of 180-200 bp, commonly called "DNA laddering". The fragmentation creates a large number of 3'-hydroxyl sites at the DNA breaks. This property is used in the APO-BRDU kit to identify apoptotic cells by labeling the 3'-hydroxyl sites with bromodeoxyuridine triphosphate (Br-dUTP). Br-dUTP is enzymatically attached to the 3-hydroxyl sites of double- or single-stranded DNA by terminal transferase (TdT). Non-apoptotic cells do not incorporate Br-dUTP due to the lack of available 3-hydroxyl sites.

  • BRAND: SIGMA
  • Adequate for: 60 cell suspensions
Info Sigma-AldrichSigma-Aldrich
Sigma-Aldrich Corp.
St. Louis, MO, USA
Customer Service: 800-325-3010
Tech Support: 800-325-5832
Fax Number: 314-771-5757
Web Site: http://www.sigmaaldrich.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. CompenFlow Flow Cytometry Compensation Kit 6 m from Molecular Probes (Invitrogen)
2. Apo-BRDU Flow Cytometry Detection Kit from CHEMICON
3. Cytomics FC 500 Series Flow Cytometry Systems from Beckman Coulter
4. Cytomics FC 500 Series Flow Cytometry Systems from Beckman Coulter
5. Cytomics FC 500 Series Flow Cytometry Systems from Beckman Coulter
6. H2A.X Phosphorylation Assay Kit (Flow Cytometry) from Upstate
7. FlowTACS Flow Cytometry Apoptosis Detection Kit from R&D Systems
8. Human Regulatory T cell (Foxp3 / CD4 / CD25) Flow Cytometry staining Kit from eBioscience
9. Apo-Direct Apoptosis Detection Kit from eBioscience
10. ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit from CHEMICON
11. ApopTag Fluorescein In Situ Apoptosis Detection Kit from CHEMICON
... fixative useful for routine procedures. Hollandes contains ... and will decalcify small bone specimens. Tissue ... stained successfully with most stains, and the ... blood cell membranes and cosinophil and endocrine ...
... useful for routine procedures. Hollandes contains formaldehyde ... will decalcify small bone specimens. Tissue that ... successfully with most stains, and the cupric ... cell membranes and cosinophil and endocrine cell ...
Chicken polyclonal antibody to Glutathione-S-Transfrease (GST)...
... informatics platform for microarray data storage, data filtering ... it forever without paying ongoing license fees. ... all the analysis tools you need to get ... statistics and normalization, all the way through to ...
Biology Products:
(Date:8/15/2017)... --  ivWatch LLC , a medical device company focused on improving ... of its ISO 13485 Certification, the global standard for medical device ... (ISO®). ... Monitoring device for the early detection of IV infiltrations. ... "This is an important milestone for ivWatch, as it ...
(Date:5/23/2017)...  Hunova, the first robotic gym for the rehabilitation and functional motor ... Genoa, Italy . The first 30 robots will be ... USA . The technology was developed and patented at the ... spin-off Movendo Technology thanks to a 10 million euro investment from entrepreneur ... ...
(Date:4/19/2017)... 19, 2017 The global military ... is marked by the presence of several large global ... by five major players - 3M Cogent, NEC Corporation, ... for nearly 61% of the global military biometric market ... the global military biometrics market boast global presence, which ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... SARASOTA, FL (PRWEB) , ... ... ... Inc. (RPS®) today announces publication of a United States multicenter, prospective clinical ... single use, disposable, point-of-care diagnostic test capable of identifying clinically significant acute ...
(Date:10/11/2017)... YORBA LINDA, CA (PRWEB) , ... October 11, ... ... adapted to upregulate any gene in its endogenous context, enabling overexpression experiments and ... activation (CRISPRa) system with small RNA guides is transformative for performing systematic gain-of-function ...
(Date:10/11/2017)... LAGUNA HILLS, Calif. , Oct. 11, 2017  SkylineDx ... London (ICR) and University of Leeds ... to risk-stratify patients with multiple myeloma (MM), in a multi-centric ... The University of Leeds is the ... UK, and ICR will perform the testing services to include ...
(Date:10/10/2017)... ... October 10, 2017 , ... ... advancing targeted antibody-drug conjugate (ADC) therapeutics, today confirmed licensing rights that give ... Liposomal Nanoparticle), a technology developed in collaboration with Children’s Hospital Los Angeles ...
Breaking Biology Technology: