Navigation Links
Flow Cytometry Kit for Apoptosis from Sigma-Aldrich

ProductsFlow Cytometry Kit for Apoptosis from Sigma-Aldrich
Company Sigma-Aldrich
Item Flow Cytometry Kit for Apoptosis
Price 
Description Application: The greater incorporation of Br-dUTP results in a stronger signal by flow cytometry when detected using a fluorescein-labeled anti-BrdU antibody. Propidium iodide/RNase A solution is included in the kit to counterstain the total DNA.
Features and Benefits: Greater incorporation of Br-dUTP, resulting in improved detection than found by using biotin- or digoxigenin-conjugated dUTP or by using fluorochrome (fluorescein or BODIPY)-conjugated deoxynucleotides.
Packaging: The kit is shipped in two parts, both wet ice. Upon receipt, store APO-PART1 at −20C and APO-PART2 at 2-4C
Preparation Principle: Br-dUTP is incorporated more readily into the DNA fragments than deoxyuridine triphosphate labeled with larger dyes such as FITC, biotin or digoxigenin. One of the biological characteristics that defines apoptosis is the degradation of genomic DNA into fragments of 180-200 bp, commonly called "DNA laddering". The fragmentation creates a large number of 3'-hydroxyl sites at the DNA breaks. This property is used in the APO-BRDU kit to identify apoptotic cells by labeling the 3'-hydroxyl sites with bromodeoxyuridine triphosphate (Br-dUTP). Br-dUTP is enzymatically attached to the 3-hydroxyl sites of double- or single-stranded DNA by terminal transferase (TdT). Non-apoptotic cells do not incorporate Br-dUTP due to the lack of available 3-hydroxyl sites.

  • BRAND: SIGMA
  • Adequate for: 60 cell suspensions
Info Sigma-AldrichSigma-Aldrich
Sigma-Aldrich Corp.
St. Louis, MO, USA
Customer Service: 800-325-3010
Tech Support: 800-325-5832
Fax Number: 314-771-5757
Web Site: http://www.sigmaaldrich.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. CompenFlow Flow Cytometry Compensation Kit 6 m from Molecular Probes (Invitrogen)
2. Apo-BRDU Flow Cytometry Detection Kit from CHEMICON
3. Cytomics FC 500 Series Flow Cytometry Systems from Beckman Coulter
4. Cytomics FC 500 Series Flow Cytometry Systems from Beckman Coulter
5. Cytomics FC 500 Series Flow Cytometry Systems from Beckman Coulter
6. H2A.X Phosphorylation Assay Kit (Flow Cytometry) from Upstate
7. FlowTACS Flow Cytometry Apoptosis Detection Kit from R&D Systems
8. Human Regulatory T cell (Foxp3 / CD4 / CD25) Flow Cytometry staining Kit from eBioscience
9. Apo-Direct Apoptosis Detection Kit from eBioscience
10. ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit from CHEMICON
11. ApopTag Fluorescein In Situ Apoptosis Detection Kit from CHEMICON
Request Info...
Detect and measure levels of mouse IgG2b antibody isotypes quickly and easily....
... Versatile state of the Art ... flask to dual 38,000 L fermentation. ... scale to production. Aseptic operation ... Batch, Fed-Batch, and Continuous Modes. ...
Request Info...
Biology Products:
(Date:3/30/2017)... , March 30, 2017 The research team ... for three-dimensional (3D) fingerprint identification by adopting ground breaking 3D fingerprint ... new realm of speed and accuracy for use in identification, crime ... affordable cost. ... A ...
(Date:3/27/2017)... , March 27, 2017  Catholic Health Services ... Management Systems Society (HIMSS) Analytics for achieving Stage ... Model sm . In addition, CHS previously earned ... hospitals using an electronic medical record (EMR). ... high level of EMR usage in an outpatient ...
(Date:3/23/2017)... Mar. 23, 2017 Research and Markets has ... Analysis & Trends - Industry Forecast to 2025" report to ... ... a CAGR of around 8.8% over the next decade to reach ... analyzes the market estimates and forecasts for all the given segments ...
Breaking Biology News(10 mins):
(Date:5/23/2017)... ... ... Bacterial biofilms, surface adherent communities of bacteria that are encased in a ... and catheter infections to gum disease and the rejection of medical implants. Though ... year, there is currently a paucity of means for preventing their formation or treating ...
(Date:5/22/2017)... ... May 22, 2017 , ... ... it is exhibiting in booth B2 at the Association for Pathology Informatics ... , In addition to demonstrating its Cancer Diagnostic Cockpit and Consultation Portal, ...
(Date:5/18/2017)... ... May 18, 2017 , ... Dr. ... 2017 at the Prince Of Wales Private Hospital. The procedure was performed on ... The patient failed conservative treatments prior to undergoing surgery. , The AxioMed viscoelastic ...
(Date:5/18/2017)... Massachusetts (PRWEB) , ... May 17, 2017 , ... ... company specializing in medical device compliance and commercialization, has just released version 9.0 ... of work into this latest version of Cockpit,” says David Cronin, CEO of ...
Breaking Biology Technology: