Navigation Links
FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH

ProductsFRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
Company Gene Bridges GmbH
Item FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT)
Price 
Description The FRT-amp-FRT cassette is designed to allow ampicillin selection in prokaryotic cells.
The prokaryotic promoter gb2 driving the gene for ampicillin resistance is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the ampicillin expression. The cassette is flanked by FRT sites for later excision by Flp recombinase. Unique NotI and XhoI sites flank the cassette for convenient cloning with restriction sites.
Using the provided PCR template one can easily create a FRT-amp-FRT cassette flanked by any other restriction sites to clone the cassette into the vector of choice. The restriction sites can be introduced by adding the corresponding sequence in the PCR primer. The template can easily be used to engineer the E.coli genome by Red/ET Recombination.
The FRT-amp-FRT cassette is not linear but plasmid based (3502 bp in size). Due to its R6K origin the plasmid cannot replicate in most E.coli strains. The PCR product can therefore be used directly for downstream applications without any further purification.
Info Gene Bridges GmbH
Commercial Centre
Im Neuenheimer Feld 584
69120 Heidekberg, Germany
Customer Service: +49 (0)6221 13708 10
Fax Number: +49 (0)6221 13708 29
Web Site: http://www.Genebridges.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
2. loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH
3. FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH
4. loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
5. loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
6. Mouse Anti-Ampicillin Monoclonal Antibody, Unconjugated, Clone 164-12-2 from Abcam
7. EasySep Biotin Selection Kit, Other from StemCell Technologies, Inc.
8. EasySep PE Selection Kit, Other from StemCell Technologies, Inc.
9. EasySep PE Selection Kit, Other, Starter Kit from StemCell Technologies, Inc.
10. EasySep FITC Selection Kit, Other from StemCell Technologies, Inc.
11. FailSafe PCR PreMix Selection Kit from EPICENTRE Biotechnologies
...
MultiWash III w/ 12-portStandard Manifold...
...
... Streptavidin Sepharose High Performance, 5 ... media used in HiTrap Streptavidin columns ... compounds.Ideal for packing into Tricorn 5/50 ... when larger scale purifications are required.Extremely ...
Biology Products:
(Date:8/23/2020)... ... August 21, 2020 , ... ... has been published and is now available on the company’s global website. ... the X-ray analysis community, presenting current news and crystallographic research. , The ...
(Date:8/21/2020)... ... , ... The 19th Annual Bio-IT World Conference & ... and IT leaders goes VIRTUAL and announces its updated event features and full ... the leadership role of keeping our life science community connected by bringing you ...
(Date:8/12/2020)... ... August 12, 2020 , ... Mosio recently announced ... for public health agencies of all sizes. With a focus on tracking symptoms ... find appropriate health care, Mosio helps public health departments automate communications and provide ...
Breaking Biology News(10 mins):
(Date:9/1/2020)... ... September 01, 2020 , ... ... and diagnostics companies, has announced the placement of Julianne Averill , CPA, ... for leading all financial operations and implementing key business strategies to accelerate Alveo’s ...
(Date:8/12/2020)... (PRWEB) , ... August 11, ... ... a biopharmaceutical cold chain validation engineering firm, is making available for free ... the need to incorporate concurrent transport simulation testing into today’s biologics licensing ...
(Date:7/31/2020)... ... July 29, 2020 , ... R3 Stem Cell International is now offering ... 50 million stem cells total, patients may choose which extremities they would like treated. ... joints (BMC Musculoskelet Disord. 2016). At R3 International, umbilical cord tissue is obtained from ...
(Date:7/31/2020)... ... , ... eSource has long been touted as the solution to high data ... the reasons it did not take off as quickly as people initially expected, and ... moving towards capturing data electronically for clinical trials and then repurposing it for downstream ...
Breaking Biology Technology: