Navigation Links
FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH

ProductsFRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
Company Gene Bridges GmbH
Item FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT)
Price 
Description The FRT-amp-FRT cassette is designed to allow ampicillin selection in prokaryotic cells.
The prokaryotic promoter gb2 driving the gene for ampicillin resistance is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the ampicillin expression. The cassette is flanked by FRT sites for later excision by Flp recombinase. Unique NotI and XhoI sites flank the cassette for convenient cloning with restriction sites.
Using the provided PCR template one can easily create a FRT-amp-FRT cassette flanked by any other restriction sites to clone the cassette into the vector of choice. The restriction sites can be introduced by adding the corresponding sequence in the PCR primer. The template can easily be used to engineer the E.coli genome by Red/ET Recombination.
The FRT-amp-FRT cassette is not linear but plasmid based (3502 bp in size). Due to its R6K origin the plasmid cannot replicate in most E.coli strains. The PCR product can therefore be used directly for downstream applications without any further purification.
Info Gene Bridges GmbH
Commercial Centre
Im Neuenheimer Feld 584
69120 Heidekberg, Germany
Customer Service: +49 (0)6221 13708 10
Fax Number: +49 (0)6221 13708 29
Web Site: http://www.Genebridges.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
2. loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH
3. FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH
4. loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
5. loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
6. Mouse Anti-Ampicillin Monoclonal Antibody, Unconjugated, Clone 164-12-2 from Abcam
7. EasySep Biotin Selection Kit, Other from StemCell Technologies, Inc.
8. EasySep PE Selection Kit, Other from StemCell Technologies, Inc.
9. EasySep PE Selection Kit, Other, Starter Kit from StemCell Technologies, Inc.
10. EasySep FITC Selection Kit, Other from StemCell Technologies, Inc.
11. FailSafe PCR PreMix Selection Kit from EPICENTRE Biotechnologies
(Date:6/4/2019)... ... ... Harper was three years old when her owners took her to the ... osteoarthritis in the joints. She was referred to a veterinary specialist to perform ... Dr. Christopher Eich of Southern California Veterinary Specialty Hospital , examined Harper and ...
(Date:6/4/2019)... , ... June 04, 2019 , ... Lajollacooks4u is delighted ... recipients. The recognition, which honors hospitality excellence, is given only to businesses that have ... Excellence for five consecutive years. , To qualify for the Certificate of ...
(Date:6/1/2019)... ... May 31, 2019 , ... The University of ... invested in BioFactura’s $6M Series B Financing Round. , “With the commitment ... who bring significant financial and business resources to bear as we advance our ...
Breaking Biology News(10 mins):
(Date:6/6/2019)... CYPRESS, Calif. (PRWEB) , ... June 05, 2019 , ... ... pharmaceutical, biotechnology and medical device industries, announced today that they will be receiving 3 ... in San Diego, CA. WCCT received 2 awards in the categories of overall Quality ...
(Date:5/31/2019)... ... 30, 2019 , ... A study released today in STEM ... the sustained release of human placental stem cell (HPSC)-derived conditioned medium (CM) to ... CM into the injured kidney, where it helped restore function and regenerate injured ...
(Date:5/14/2019)... N.J. (PRWEB) , ... May 13, 2019 , ... ... custom built, helium-based leak testing instruments for the Pharmaceutical, Biotechnology, Medical Device and ... Heliumleak.com company website. The redesigned website aims to improve LDA’s ability to communicate ...
(Date:5/4/2019)... ... May 03, 2019 , ... Diopsys Inc, ... Diopsys® ffERG/Photopic Negative Response vision test, a new full field electroretinography (ffERG) ... estimated that 6.3 million Americans will have glaucoma, with black Americans having the ...
Breaking Biology Technology: