Navigation Links
FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH

ProductsFRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
Company Gene Bridges GmbH
Item FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT)
Price 
Description The FRT-PGK-gb2-neo-FRT template is designed to allow neomycin/kanamycin selection in prokaryotic and eukaryotic cells.
The FRT-PGK-gb2-neo-FRT template encodes the neomycin/kanamycin resistance gene which combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eukaryotic promoter (PGK) for expression of neomycin resistance in mammalian cells.
The prokaryotic promoter gb2 is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression. The cassette is flanked by FRT sites for later excision by Flp recombinase. Unique NotI and XhoI sites flank the cassette for convenient cloning with restriction sites.
Using the provided PCR template one can easily create a FRT-PGK-gb2-neo-FRT cassette flanked by any restriction sites to clone the cassette into the vector of choice. The restriction sites can be introduced by adding the corresponding sequence in the PCR primer. The template can easily be used to generate targeting constructs mediated by a single Red/ET Recombination step.
The FRT-PGK-gb2-neo-FRT template is not linear but plasmid based (3446bp in size). Due to its R6K origin it can not replicate in most E. coli strains. The PCR product can therefore be used directly for downstream applications without any further purification.
Info Gene Bridges GmbH
Commercial Centre
Im Neuenheimer Feld 584
69120 Heidekberg, Germany
Customer Service: +49 (0)6221 13708 10
Fax Number: +49 (0)6221 13708 29
Web Site: http://www.Genebridges.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH
2. FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH
3. EasySep Biotin Selection Kit, Other from StemCell Technologies, Inc.
4. EasySep PE Selection Kit, Other from StemCell Technologies, Inc.
5. EasySep PE Selection Kit, Other, Starter Kit from StemCell Technologies, Inc.
6. EasySep FITC Selection Kit, Other from StemCell Technologies, Inc.
7. loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
8. FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
9. loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
10. FailSafe PCR PreMix Selection Kit from EPICENTRE Biotechnologies
11. Trans-Blot Plus Gel and Cassette Assembly Tray from Bio-Rad
M-PER from Pierce Biotechnology, Inc.
RNase AWAY(r) from Sigma-Aldrich
ProteoMass Cytochrome c MALDI-MS Standard from Sigma-Aldrich
...er of modifications of Grace's medium were develop...1970's. Many of these modifications were developed...from the cabbage looper,Tricoplusia ni. This formu...e medium when properly supplemented has been found...
Biology Products:
(Date:11/25/2009)... Soil is the linchpin of the environment, where at... that, many students see soil as "just dirt" a pl... educators are challenged with the task of helping...portance of soil in the environment. , A collab...teachers at University of Nebraska-Lincoln, Oregon...
(Date:11/24/2009)...een prescribed by traditional healers in Brazil to...ach pain to fever and flu. , Now for the first ti...able to scientifically prove the pain relieving pr...razilian mint. , Testing this ancient South Ameri...ela Rocha was able to show that when prepared as a...
(Date:11/24/2009)...labby arteries from collapsing have been true life... longer needed -- once the arteries are strengthen...ad no choice but to leave them in place. , Prof....ent of Biomedical Engineering has developed a new ...here they,re needed, then dissolves. , "Our new ...
Breaking Biology News(10 mins):It's not just dirt! 2Got a pain? -- Have a cup of Brazilian mint 2A coating for life 2Eliminate the Blues with Color Therapy 54599 1Eliminate the Blues with Color Therapy 54599 2Eliminate the Blues with Color Therapy 54599 3New nanolaser key to future optical computers and technologies 13581 1New nanolaser key to future optical computers and technologies 13581 2New nanolaser key to future optical computers and technologies 13581 3Inherited Risk Factors Increase Odds of Developing Childhood Acute Lymphoblastic Leukemia 54596 1Inherited Risk Factors Increase Odds of Developing Childhood Acute Lymphoblastic Leukemia 54596 2Inherited Risk Factors Increase Odds of Developing Childhood Acute Lymphoblastic Leukemia 54596 3Inherited Risk Factors Increase Odds of Developing Childhood Acute Lymphoblastic Leukemia 54596 4
...Foundation, through its Autism Research Initiative...h the Rutgers University Cell and DNA Repository (...r autism studies. The samples will be collected fr...d. , The Simons Simplex Collection, as this proj...r a new and different line of research into the ge...
...September 04, 2007 - The term "biotechnology" elic...ar and hostility. Alan McHughen from the Universit... at large the current state public knowledge of bi...cy and the role of the media. How is coexistence d...agricultural biotech only big companies or also t...
...h showing that dog-walking in bushland significant...support to bans against the practice in sensitive ...uments and debate about the ecological impacts of ...d because experimental evidence has been lacking. ...e dogs as predators and avoid dog-walking areas is...
Other Biology News:Rutgers Genetics receives $7.8 million for autism research 2Rutgers Genetics receives $7.8 million for autism research 3How the public perceives biotech 2A dog in the hand scares birds in the bush 2
(Date:11/24/2009)...KJAVIK, Iceland, November 24 /PRNewswire-FirstCall...nnounced the approval of all of its,"first day" mo...t of Delaware,(the "Bankruptcy Court"). deCODE las... Chapter 11 of the United States Bankruptcy Code w...sued by the Bankruptcy Court allow the company to ...
(Date:11/24/2009)...Newswire/ -- Blue Marble Energy Corp. announced to...ment Authority (OPDA) were recently awarded $2 mil...talization Board (CERB) in a private/public partne...chemical biorefinery in Lincoln County, WA. ,...velopment of our biorefinery and bring over 70 gre...
(Date:11/24/2009)...r 24 Shire plc (LSE: SHP...ceutical company, today,announced that it has subm...elaglucerase alfa, the company,s enzyme replacemen... 1 Gaucher disease. This is the third marketing,ap...mitted, with previous,submissions in the United St...
(Date:11/24/2009)...m Connects Customers with Partners Validated to Pr...ek Products , Ottawa, ON ...ing provider of products for biological sample col...unced its new global partner program. The program ...partner community and is designed to help partners...
Breaking Biology Technology:deCODE genetics, Inc. Announces Approval of First Day Motions by Bankruptcy Court 2deCODE genetics, Inc. Announces Approval of First Day Motions by Bankruptcy Court 3Blue Marble Energy, OPDA Awarded $2M by WA's Community Economic Revitalization Board 2Shire Submits European Marketing Authorization Application (MAA) for velaglucerase alfa for the Treatment of Type 1 Gaucher Disease 2Shire Submits European Marketing Authorization Application (MAA) for velaglucerase alfa for the Treatment of Type 1 Gaucher Disease 3Shire Submits European Marketing Authorization Application (MAA) for velaglucerase alfa for the Treatment of Type 1 Gaucher Disease 4DNA Genotek Launches Global Partner Program 2DNA Genotek Launches Global Partner Program 3