Navigation Links
FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH

ProductsFRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
Company Gene Bridges GmbH
Item FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT)
Price 
Description The FRT-PGK-gb2-neo-FRT template is designed to allow neomycin/kanamycin selection in prokaryotic and eukaryotic cells.
The FRT-PGK-gb2-neo-FRT template encodes the neomycin/kanamycin resistance gene which combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eukaryotic promoter (PGK) for expression of neomycin resistance in mammalian cells.
The prokaryotic promoter gb2 is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression. The cassette is flanked by FRT sites for later excision by Flp recombinase. Unique NotI and XhoI sites flank the cassette for convenient cloning with restriction sites.
Using the provided PCR template one can easily create a FRT-PGK-gb2-neo-FRT cassette flanked by any restriction sites to clone the cassette into the vector of choice. The restriction sites can be introduced by adding the corresponding sequence in the PCR primer. The template can easily be used to generate targeting constructs mediated by a single Red/ET Recombination step.
The FRT-PGK-gb2-neo-FRT template is not linear but plasmid based (3446bp in size). Due to its R6K origin it can not replicate in most E. coli strains. The PCR product can therefore be used directly for downstream applications without any further purification.
Info Gene Bridges GmbH
Commercial Centre
Im Neuenheimer Feld 584
69120 Heidekberg, Germany
Customer Service: +49 (0)6221 13708 10
Fax Number: +49 (0)6221 13708 29
Web Site: http://www.Genebridges.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH
2. FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH
3. EasySep Biotin Selection Kit, Other from StemCell Technologies, Inc.
4. EasySep PE Selection Kit, Other from StemCell Technologies, Inc.
5. EasySep PE Selection Kit, Other, Starter Kit from StemCell Technologies, Inc.
6. EasySep FITC Selection Kit, Other from StemCell Technologies, Inc.
7. loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
8. FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
9. loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
10. FailSafe PCR PreMix Selection Kit from EPICENTRE Biotechnologies
11. Trans-Blot Plus Gel and Cassette Assembly Tray from Bio-Rad
... Cyno serum is collected off the clot from fasted ... age. Available Anticoagulants: N-02: ... K2EDTA N-08: Potassium Oxalate N-10: ... ACD N-07: CPD N-09: K3EDTA ...
3'-O-Methylguanosine 5'-Triphosphate, Sodium, 1 umol. Category: Nucleotides & Enzymes & Biochemicals, Nucleotides, Additional Nucleotide Products....
Recombinant Viral CCI/Fc Chimera, CF...
... ml. Improves reproducibility and quality ... streaking.Eliminates extra spots caused by ... throughout entire 2-D runs, ensuring ... for both analytical and preparative ...
Biology Products:
(Date:5/16/2017)... DALLAS , May 16, 2017   ... for health organizations, and MD EMR Systems ... certified development partner for GE, have established a ... Patient Portal product and the GE Centricity™ products, ... Centricity EMR. These new integrations ...
(Date:4/19/2017)... , April 19, 2017 The ... vendor landscape is marked by the presence of several ... however held by five major players - 3M Cogent, ... companies accounted for nearly 61% of the global military ... companies in the global military biometrics market boast global ...
(Date:4/13/2017)... 2017 UBM,s Advanced Design and Manufacturing event ... emerging and evolving technology through its 3D Printing and ... alongside the expo portion of the event and feature ... focused on trending topics within 3D printing and smart ... event will take place June 13-15, 2017 at the Jacob ...
Breaking Biology News(10 mins):
(Date:7/20/2017)... , ... July 20, 2017 , ... VIC Technology ... joining the company’s board of directors. This addition continues to strengthen and diversify ... Calvin Goforth, CEO and Chairman. “He is a highly accomplished business executive with a ...
(Date:7/20/2017)... ... July 20, 2017 , ... Corporate Directors Forum is ... 27th annual Director of the Year Awards. , The awards will be presented Thursday, ... This annual event celebrates directors who have made significantly positive contributions in the boardrooms ...
(Date:7/18/2017)... , ... July 18, 2017 , ... Sourcing custom glass ... your needs and has the capabilities to properly execute your job can take many ... is a sourcing portal designed to showcase the company’s capabilities and core ...
(Date:7/18/2017)... San Antonio, Texas (PRWEB) , ... July 18, 2017 , ... ... that can affect patients across the region. Nationally, summer is a struggle for community ... This is why the San Antonio Chamber of Commerce is teaming up ...
Breaking Biology Technology: