Navigation Links
FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH

ProductsFRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
Company Gene Bridges GmbH
Item FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT)
Price 
Description The FRT-PGK-gb2-neo-FRT template is designed to allow neomycin/kanamycin selection in prokaryotic and eukaryotic cells.
The FRT-PGK-gb2-neo-FRT template encodes the neomycin/kanamycin resistance gene which combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eukaryotic promoter (PGK) for expression of neomycin resistance in mammalian cells.
The prokaryotic promoter gb2 is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression. The cassette is flanked by FRT sites for later excision by Flp recombinase. Unique NotI and XhoI sites flank the cassette for convenient cloning with restriction sites.
Using the provided PCR template one can easily create a FRT-PGK-gb2-neo-FRT cassette flanked by any restriction sites to clone the cassette into the vector of choice. The restriction sites can be introduced by adding the corresponding sequence in the PCR primer. The template can easily be used to generate targeting constructs mediated by a single Red/ET Recombination step.
The FRT-PGK-gb2-neo-FRT template is not linear but plasmid based (3446bp in size). Due to its R6K origin it can not replicate in most E. coli strains. The PCR product can therefore be used directly for downstream applications without any further purification.
Info Gene Bridges GmbH
Commercial Centre
Im Neuenheimer Feld 584
69120 Heidekberg, Germany
Customer Service: +49 (0)6221 13708 10
Fax Number: +49 (0)6221 13708 29
Web Site: http://www.Genebridges.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH
2. FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH
3. EasySep Biotin Selection Kit, Other from StemCell Technologies, Inc.
4. EasySep PE Selection Kit, Other from StemCell Technologies, Inc.
5. EasySep PE Selection Kit, Other, Starter Kit from StemCell Technologies, Inc.
6. EasySep FITC Selection Kit, Other from StemCell Technologies, Inc.
7. loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
8. FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
9. loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
10. FailSafe PCR PreMix Selection Kit from EPICENTRE Biotechnologies
11. Trans-Blot Plus Gel and Cassette Assembly Tray from Bio-Rad
BD BioCoat Collagen I 35 mm Culture Dishes, tissue-culture treated polystyrene with a uniform application of rat tail collagen type I....
... CLS number is a new product ... product number. If showing no availability ... Sigma-Aldrich number (C6671) or contact customer ... yes Surface area 148 cm 2 ...
... This CLS number is a new ... product number. If showing no availability yet, ... (Z71,371-6) or contact customer service for assistance. ... Comp Dim: surface area 500 cm ...
BD BioCoat Collagen IV 150 mm Culture Dishes...
Biology Products:
(Date:5/24/2016)... 2016 Ampronix facilitates superior patient care by providing unparalleled technology to leaders ... is the latest premium product recently added to the range of products distributed by ... ... ... LCD Medical Display- Ampronix News ...
(Date:5/9/2016)... DUBAI , UAE, May 9, 2016 ... choice when it comes to expanding freedom for high ... Even in today,s globally connected world, there ... online conferencing system could ever duplicate sealing your deal ... are obtaining second passports by taking advantage of citizenship ...
(Date:4/26/2016)... BANGALORE, India and LONDON ... Infosys Finacle, part of EdgeVerve Systems, a ... ), and Onegini today announced a partnership to ... banking solutions.      (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ... banks to provide their customers enhanced security to ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... /PRNewswire/ - BIOREM Inc. (TSX-V: BRM) ("Biorem" or "the Company") ... shareholders, Clean Technology Fund I, LP and Clean Technology ... based venture capital funds which together hold approximately 59% ... diluted, as converted basis), that they have entered into ... holdings in Biorem to TUS Holdings Co. Ltd. ("TUS") ...
(Date:6/27/2016)... ... June 27, 2016 , ... Rolf ... join the faculty of the University of North Carolina Kenan-Flagler Business School ... and entrepreneurship at UNC Kenan-Flagler, with a focus on the school’s international efforts, ...
(Date:6/24/2016)... ... 2016 , ... While the majority of commercial spectrophotometers and fluorometers use the ... models are higher end machines that use the more unconventional z-dimension of 20mm. ... the bottom of the cuvette holder. , FireflySci has developed several Agilent flow ...
(Date:6/23/2016)... , June 23, 2016 /PRNewswire/ - FACIT ... Ontario biotechnology company, Propellon Therapeutics ... development and commercialization of a portfolio of first-in-class ... Epigenetic targets such as WDR5 represent an exciting ... significantly in precision medicine for cancer patients. Substantial ...
Breaking Biology Technology: