Navigation Links
FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH

ProductsFRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
Company Gene Bridges GmbH
Item FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT)
Price 
Description The FRT-PGK-gb2-neo-FRT template is designed to allow neomycin/kanamycin selection in prokaryotic and eukaryotic cells.
The FRT-PGK-gb2-neo-FRT template encodes the neomycin/kanamycin resistance gene which combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eukaryotic promoter (PGK) for expression of neomycin resistance in mammalian cells.
The prokaryotic promoter gb2 is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression. The cassette is flanked by FRT sites for later excision by Flp recombinase. Unique NotI and XhoI sites flank the cassette for convenient cloning with restriction sites.
Using the provided PCR template one can easily create a FRT-PGK-gb2-neo-FRT cassette flanked by any restriction sites to clone the cassette into the vector of choice. The restriction sites can be introduced by adding the corresponding sequence in the PCR primer. The template can easily be used to generate targeting constructs mediated by a single Red/ET Recombination step.
The FRT-PGK-gb2-neo-FRT template is not linear but plasmid based (3446bp in size). Due to its R6K origin it can not replicate in most E. coli strains. The PCR product can therefore be used directly for downstream applications without any further purification.
Info Gene Bridges GmbH
Commercial Centre
Im Neuenheimer Feld 584
69120 Heidekberg, Germany
Customer Service: +49 (0)6221 13708 10
Fax Number: +49 (0)6221 13708 29
Web Site: http://www.Genebridges.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH
2. FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH
3. EasySep Biotin Selection Kit, Other from StemCell Technologies, Inc.
4. EasySep PE Selection Kit, Other from StemCell Technologies, Inc.
5. EasySep PE Selection Kit, Other, Starter Kit from StemCell Technologies, Inc.
6. EasySep FITC Selection Kit, Other from StemCell Technologies, Inc.
7. loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
8. FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
9. loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
10. FailSafe PCR PreMix Selection Kit from EPICENTRE Biotechnologies
11. Trans-Blot Plus Gel and Cassette Assembly Tray from Bio-Rad
... of long double stranded RNA (dsRNA) has ... a diverse group of organisms as well ... leads to the inhibition of protein expression ... target messenger RNA (mRNA). Attempts to induce ...
... easy-to-use siRNA transfection reagent that has been ... a variety of cell lines. jetSI-ENDO is ... straightforward protocol. High levels of gene silencing ... of 20 to 100 nM of siRNA ...
... stranded RNA (dsRNA) has been shown to ... of organisms as well as insect cells ... inhibition of protein expression by utilizing sequence-specific, ... (mRNA). Attempts to induce RNA interference using ...
... stain is used to distinguish collagen from ... Staining System. The Trichrome stain is often ... muscle and to identify an increase in ... stain, muscle is stained red, collagen - ...
Biology Products:
(Date:8/14/2019)... , ... August 13, 2019 , ... ... Team, Division of Molecular Pathology, Institute of Cancer Research (ICR) in a ... to learn about the process of biomarker and companion diagnostic development including machine ...
(Date:8/6/2019)... ... ... Keck Graduate Institute (KGI) President Sheldon Schuster has announced Martin Zdanowicz as the ... is highly regarded as an innovative leader who prioritizes student success and faculty development,” ... build on the success of the KGI School of Pharmacy and Health Sciences.” , ...
(Date:8/1/2019)... ... 01, 2019 , ... The GENSPEED platform is a compact, fast and simple ... developed with over 100 units deployed in the field for multiple applications. “Beyond ... with axiVEND will give us a foothold in the USA to better support other ...
Breaking Biology News(10 mins):
(Date:8/6/2019)... ... August 06, 2019 , ... ... joined forces in launching the biweekly SDBN BUZZ podcast focused on connecting the ... podcast will also serve to promote the region and attract external employers, investors, ...
(Date:8/6/2019)... ... 05, 2019 , ... Advanced Clinical, a clinical research services ... by PharmaVOICE magazine, earning him a prestigious spot on the 2019 PharmaVOICE 100 ... , As the executive vice president of business development and marketing at Advanced ...
(Date:8/4/2019)... ... August 02, 2019 , ... Cirtec Medical Corporation, a ... today announced that it has begun construction on a 30,000 sq. ft. manufacturing ... from San Jose. , The facility, which is expected to be fully operational ...
(Date:7/26/2019)... ... July 25, 2019 , ... LeadingBiotech , ... dialogue, today announces its Boston BD conference to be held on ... Biotech Week Boston , the event provides an opportunity for attendees to leverage ...
Breaking Biology Technology: