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FAM Poly Caspase Assay Kit from B-Bridge International

ProductsFAM Poly Caspase Assay Kit from B-Bridge International
Company B-Bridge International
Item FAM Poly Caspase Assay Kit
Description The Poly-Caspases FLICA Apoptosis Detection Kit contains a green fluorescent-labeled inhibitor, FAM-VAD-FMK, which is a carboxyfluorescein (FAM) derivative of valylalanylaspartic acid (VAD) fluoromethyl ketone (FMK), a potent inhibitor of caspase activity. When added to a population of cells, the FAM-VAD-FMK FLICA probe enters each cell and covalently binds to a reactive cysteine residue that resides on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. Because the FAM-VAD-FMK FLICA reagent becomes covalently coupled to the enzyme, it is retained within the cell, while any unbound FAM-VAD-FMK FLICA reagent will diffuse out of the cell and is washed away. The remaining green fluorescent signal is a direct measure of the number of active caspase enzymes that were present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by 96-well-plate based fluorometry, fluorescence microscopy, or flow cytometry.
Info B-Bridge InternationalB-Bridge International
320 Logue Avenue
Mountain View, CA 94043

Customer Service: 650-969-7727
Fax Number: 650-969-7737
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NOTE: Price information is approximate list price and actual prices may vary.

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