|Products||EcoR I from USB Corp.|
|Description|| Source: Escherichia coli RY 13|
Heat inactivation at 60C for 15 min or ethanol precipitation.
10mM Tris-HCl (pH 7.5), 100mM KCl, 0.1mM EDTA, 1.0mM DTT, 0.15% Triton X-100, 0.01% BSA, 50% glycerol.
Shipping and Storage:
Shipped on dry ice. Store at -20C.
One unit is defined as the amount of enzyme required to digest 1 g of lambda DNA completely in 50 l of the reaction mixture at 37C for 1 hour.
Standard Conc.[PN 70555]: 8 - 20 un/l
High Conc.[PN 70658]: 30 - 60 un/l
After 10-fold over digestion with this enzyme, the DNA fragments are ligated with T4 DNA Ligase and re-digested with this enzyme.
Includes (1 ml each): 10X High Buffer and 10X Loading Buffer. A USB Floatboat will be sent free with every USB Restriction Enzyme.
|Info|| USB Corp.|
26111 Miles Road
Cleveland Ohio 44128
Fax Number: 216.464.5075
Web Site: http://www.usbweb.com