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EcoR I from USB Corp.

ProductsEcoR I from USB Corp.
Company USB Corp.
Item EcoR I
Price 
Description Source: Escherichia coli RY 13
5'G AATTC3'
3'CTTAA G5'
Inactivation Conditions:
Heat inactivation at 60C for 15 min or ethanol precipitation.
Storage Conditions:
10mM Tris-HCl (pH 7.5), 100mM KCl, 0.1mM EDTA, 1.0mM DTT, 0.15% Triton X-100, 0.01% BSA, 50% glycerol.
Shipping and Storage:
Shipped on dry ice. Store at -20C.
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 g of lambda DNA completely in 50 l of the reaction mixture at 37C for 1 hour.
Concentration:
Standard Conc.[PN 70555]: 8 - 20 un/l
High Conc.[PN 70658]: 30 - 60 un/l
Ligation-Redigestion:
After 10-fold over digestion with this enzyme, the DNA fragments are ligated with T4 DNA Ligase and re-digested with this enzyme.
Includes (1 ml each): 10X High Buffer and 10X Loading Buffer. A USB Floatboat will be sent free with every USB Restriction Enzyme.
Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128


Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-216-245-1829
Customer Service: 216.765.5000
Fax Number: 216.464.5075
Web Site: http://www.usbweb.com
NOTE: Price information is approximate list price and actual prices may vary.

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