Navigation Links
Dithio-bis(2-nitrobenzoic acid) BioChemica from AppliChem GmbH

ProductsDithio-bis(2-nitrobenzoic acid) BioChemica from AppliChem GmbH
Company AppliChem GmbH
Item Dithio-bis(2-nitrobenzoic acid) BioChemica
Price 
Description Synonym: Dinitro-5,5'-dithiodibenzoic acid , DTNB , Ellman's reagent


*M: 396.36 g/mol
*CAS-No.: 69-78-3
*Storage: RT

When an enzyme-catalyzed reaction creates a product with a reactive grouping not present in the substrate, it may be possible to include a reagent which will react directly with the product to form a chromophore. The chromogenic reactant should not interfere with the enzymic reaction. 5,5'-Dithiobis-2-nitrobenzoic acid (DTNB, Ellman's reagent) reacts with free thiols in an exchange reaction that produces the yellow 4-nitrothiolate anion. For the assay of acetylcholine esterase (E.C. 3.1.1.7), acetylthiocholine iodide (see product no. A3754) is used as a synthetic substrate, replacing the natural substrate acetylcholine. This enzyme hydrolyses the substrate to yield acetate and thiocholine. The free thiol group of thiocholine reacts with DTNB included in the assay mixture, producing the yellow 4-nitrothiolate. The release of this yellow anion is followed at 412 nm. The assay may be performed at 30C in 0.1 M sodium phosphate buffer with 10 mM DTNB and 12.5 mM acetylthiocholine iodide (1). Stability: Solutions of DTNB are not very stable. You may keep them refrigerated for 3-4 days. Freezing improves stability. It is recommended to prepare the solution fresh each time.

Literature:
*(1) John, R.A. (1993) Photometric assays in Enzyme Assays: A Practical Approach p. 81 (Eisenthal, R. & Danson, M.F. eds.) Oxford University Press; Oxford, New York, Tokyo.

Info AppliChem GmbHAppliChem GmbH
AppliChem GmbH
Ottoweg 4
D-64291 Darmstadt
Germany
Tel: +49 (0)6151 / 9357-0
Fax: +49 (0)6151 / 9357-11

AppliChem Inc.
505 Highland Avenue
Cheshire, CT 06410 U.S.A.
Tel: (800) 620-7783
Fax: (800) 823-9903

Customer Service: +49 (0)6151 / 9357-0, 1 (800) 620-7783
Fax Number: +49 (0)6151 / 9357-11, 1 (800) 823-9903
Web Site: http://www.applichem.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. 55-dithiobis-(2-nitrobenzoic acid) (DTNB; Ellmans reagent) from Molecular Probes (Invitrogen)
2. Schneiders Drosophila - Insect medium from AppliChem GmbH
3. TNM-FH - Insect medium (modified Graces Insect medium) from AppliChem GmbH
4. Denhardts powder mixture (for 50X stock solution) from AppliChem GmbH
5. CromoCenAGN SC - Powder with supplement A5012 from AppliChem GmbH
6. Leukocytes, human - sonicated from AppliChem GmbH
7. Taq DNA Polymerase -Klein Taq from AppliChem GmbH
Borosilicate Glass. Certified for use with the Beacon 2000 Systems, 1,000 tubes/case...
Whole blood is collected from healthy guinea pigs of either sex from US source which are free of visible diseases at time of blood collection. Hemolytic titer: CH50 = 435 units...
... 96 wells. ELISA.Specific for total MMP-3: Recognizes ... cross-reactivity with MMP-1, -2, -9. Range: 3.75-120 ... cell culture supernatant, serum, plasma and tissue ... C. Category: Drug Screening & Cellular ...
... Steady gravity-fed delivery of buffer ... animal perfusion. Rat version. In Vivo ... results--Steady gravity-fed delivery of buffer and ... Easy to setup, use and clean--Complete ...
Biology Products:
(Date:3/27/2017)... ROCKVILLE CENTRE, N.Y. , March 27, 2017 ... by Healthcare Information and Management Systems Society (HIMSS) ... Analytics Outpatient EMR Adoption Model sm . In ... top 12% of U.S. hospitals using an electronic ... recognized CHS for its high level of EMR ...
(Date:3/24/2017)... -- Research and Markets has announced the addition of ... - Industry Forecast to 2025" report to their offering. ... The Global Biometric Vehicle ... around 15.1% over the next decade to reach approximately $1,580 million ... estimates and forecasts for all the given segments on global as ...
(Date:3/23/2017)... Research and Markets has announced the addition of the ... to 2025" report to their offering. ... The Global Vehicle Anti-Theft System Market ... the next decade to reach approximately $14.21 billion by 2025. ... for all the given segments on global as well as regional ...
Breaking Biology News(10 mins):
(Date:10/10/2017)... ... October 10, 2017 , ... ... program has won a US2020 STEM Mentoring Award. Representatives of the FirstHand program ... in Volunteer Experience from US2020. , US2020’s mission is to change the trajectory ...
(Date:10/10/2017)... ... October 10, 2017 , ... ... 13 prestigious awards honoring scientists who have made outstanding contributions ... scheduled symposium during Pittcon 2018, the world’s leading conference and exposition for laboratory ...
(Date:10/9/2017)... , ... October 09, 2017 ... ... on October 5, 2017, in the medical journal, Epilepsia, Brain Sentinel’s SPEAC® ... gold standard, video EEG, in detecting generalized tonic-clonic seizures (GTCS) using surface ...
(Date:10/6/2017)... ... October 06, 2017 , ... On Tuesday, October 24th, ... INSIGhT, the first-ever adaptive clinical trial for glioblastoma (GBM). The featured speaker will ... free and open to the public, but registration is required. , WHAT: ...
Breaking Biology Technology: