Navigation Links
Dithio-bis(2-nitrobenzoic acid) BioChemica from AppliChem GmbH

ProductsDithio-bis(2-nitrobenzoic acid) BioChemica from AppliChem GmbH
Company AppliChem GmbH
Item Dithio-bis(2-nitrobenzoic acid) BioChemica
Price 
Description Synonym: Dinitro-5,5'-dithiodibenzoic acid , DTNB , Ellman's reagent


*M: 396.36 g/mol
*CAS-No.: 69-78-3
*Storage: RT

When an enzyme-catalyzed reaction creates a product with a reactive grouping not present in the substrate, it may be possible to include a reagent which will react directly with the product to form a chromophore. The chromogenic reactant should not interfere with the enzymic reaction. 5,5'-Dithiobis-2-nitrobenzoic acid (DTNB, Ellman's reagent) reacts with free thiols in an exchange reaction that produces the yellow 4-nitrothiolate anion. For the assay of acetylcholine esterase (E.C. 3.1.1.7), acetylthiocholine iodide (see product no. A3754) is used as a synthetic substrate, replacing the natural substrate acetylcholine. This enzyme hydrolyses the substrate to yield acetate and thiocholine. The free thiol group of thiocholine reacts with DTNB included in the assay mixture, producing the yellow 4-nitrothiolate. The release of this yellow anion is followed at 412 nm. The assay may be performed at 30C in 0.1 M sodium phosphate buffer with 10 mM DTNB and 12.5 mM acetylthiocholine iodide (1). Stability: Solutions of DTNB are not very stable. You may keep them refrigerated for 3-4 days. Freezing improves stability. It is recommended to prepare the solution fresh each time.

Literature:
*(1) John, R.A. (1993) Photometric assays in Enzyme Assays: A Practical Approach p. 81 (Eisenthal, R. & Danson, M.F. eds.) Oxford University Press; Oxford, New York, Tokyo.

Info AppliChem GmbHAppliChem GmbH
AppliChem GmbH
Ottoweg 4
D-64291 Darmstadt
Germany
Tel: +49 (0)6151 / 9357-0
Fax: +49 (0)6151 / 9357-11

AppliChem Inc.
505 Highland Avenue
Cheshire, CT 06410 U.S.A.
Tel: (800) 620-7783
Fax: (800) 823-9903

Customer Service: +49 (0)6151 / 9357-0, 1 (800) 620-7783
Fax Number: +49 (0)6151 / 9357-11, 1 (800) 823-9903
Web Site: http://www.applichem.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. 55-dithiobis-(2-nitrobenzoic acid) (DTNB; Ellmans reagent) from Molecular Probes (Invitrogen)
2. Schneiders Drosophila - Insect medium from AppliChem GmbH
3. TNM-FH - Insect medium (modified Graces Insect medium) from AppliChem GmbH
4. Denhardts powder mixture (for 50X stock solution) from AppliChem GmbH
5. CromoCenAGN SC - Powder with supplement A5012 from AppliChem GmbH
6. Leukocytes, human - sonicated from AppliChem GmbH
7. Taq DNA Polymerase -Klein Taq from AppliChem GmbH
Request Info...
Mouse monoclonal [3G131] to Desmoglein 1 ( Abpromise for all tested applications). entrezGeneID: 1828 SwissProtID: Q02413...
TCP-1 alpha (H-110)...
Synphilin 1 Immunogen: Synthetic peptide corresponding to residues S(829) L E L N G E K D K D K G R T L Q R T(847) of human Synphilin 1. Storage: -20 C, Avoid Freeze/Thaw Cycles...
Biology Products:
(Date:12/17/2014)... RALEIGH, N.C. , Dec. 16, 2014 Valencell, ... licensed its PerformTek biometric technology to industry leaders such ... highly accurate, clinically validated, biometric wearable products. These products ... 6-9 in Las Vegas . ...
(Date:12/11/2014)... and Markets , http://photos.prnewswire.com/prnh/20130307/600769 ... the addition of the "Biometrics Market in the ... One major trend emerging in this market is ... is important to upgrade biometric solutions to the latest ...
(Date:12/10/2014)... been here before: you desperately need to sign into your online ... answer to your secret question. What,s your dog,s birthday? Who was ... , a digital infrastructure security company, launches the app ... with usernames, passwords and PINs – 1U TM . ...
Breaking Biology News(10 mins):Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2Biometrics Market in the APAC Region 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3
... BOULDER--The use of prescribed burns to manage Western ... footprint. A new study finds that such burns, often ... bigger trees, release substantially less carbon dioxide emissions than ... prescribed burns can be an important piece of a ...
... DC, March 16, 2010 A new study ... research institutions states that forest protection offers one ... to combat climate change. The study, "Indigenous Lands, ... in PLoS Biology , a peer-reviewed scientific ...
... UCSF researchers have discovered that a key cellular defect ... can lead to cancer susceptibility. The scientists also found ... in correcting this defect. According to the study ... fight against cancer and could affect treatment of lymphoma ...
Cached Biology News:Prescribed burns may help reduce US carbon footprint 2Prescribed burns may help reduce US carbon footprint 3Study highlights forest protected areas as a critical strategy for slowing climate change 2Blocking cancer in its path: New cellular defect discovered 2Blocking cancer in its path: New cellular defect discovered 3
(Date:1/22/2015)... Wilmington, DE (PRWEB) January 22, 2015 ... the Pistoia Alliance, which helps companies check the legal ... set to expand to China. , As their ... companies are globally adopting software solutions built as a ...
(Date:1/22/2015)... Nueva Jersey , 22 de enero de 2015 ... Research abre hoy su llamada a nominaciones para 2015. ... científica ha hecho, o tiene el potencial para hacer, ... Las nominaciones se aceptarán hasta el 15 de marzo ...
(Date:1/22/2015)... , a precision medicine software company that has developed a ... genomic, imaging and other biomedical data in research and clinical ... CEO of Aspera, an IBM Company, to its board as ... "We are pleased to welcome Michelle to ...
(Date:12/24/2014)... , Dec. 24, 2014  United Therapeutics Corporation (NASDAQ: ... MDT ) has submitted a pre-market approval ... for the use of Medtronic,s SynchroMed ® II ... for use with United Therapeutics, Remodulin ® (treprostinil) ...
Breaking Biology Technology:Global Compliance Service for Controlled Substances to Expand to China 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4GenoSpace Expands Board with Appointment of Michelle Munson 2United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 2United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 3United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 4