| Products | DNA Exonuclease III , E. coli from EPICENTRE Biotechnologies |
| Company | EPICENTRE Biotechnologies |
| Item | DNA Exonuclease III , E. coli |
| Price | $48.00 |
| Description | Exonuclease III digests duplex DNA in a 3'-> 5' direction from a blunt end, 5'-overhang or nick, producing stretches of single-stranded DNA. Under defined reaction conditions, DNA degradation by Exonuclease III proceeds at a uniform rate yielding predictable and reproducible results. Because the rate of exonucleolytic excision of deoxyribonucleotides by Exonuclease III is dependent upon reaction factors including temperature, ionic strength, template sequence, and enzyme-to-DNA ratios, each template must be optimized using sample digestions to achieve the desired excision rate. Exonuclease III is not active on 3'-protruding ends of 4 bases or more in length, single-stranded DNA, or on thioester-linked nucleotides. The enzyme also has intrinsic RNase H, 3'-DNA phosphatase, and apurinic DNA endonuclease activities. |
| Info | EPICENTRE Biotechnologies726 Post Road Madison, WI 53713 USA Customer Service: 800-284-8474 Tech Support: 800-284-8474 Fax Number: 608-442-6114 Web Site: http://www.epibio.com |