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DNA Exonuclease III , E. coli from EPICENTRE Biotechnologies

ProductsDNA Exonuclease III , E. coli from EPICENTRE Biotechnologies
Company EPICENTRE Biotechnologies
Item DNA Exonuclease III , E. coli
Price $48.00
Description Exonuclease III digests duplex DNA in a 3'-> 5' direction from a blunt end, 5'-overhang or nick, producing stretches of single-stranded DNA. Under defined reaction conditions, DNA degradation by Exonuclease III proceeds at a uniform rate yielding predictable and reproducible results. Because the rate of exonucleolytic excision of deoxyribonucleotides by Exonuclease III is dependent upon reaction factors including temperature, ionic strength, template sequence, and enzyme-to-DNA ratios, each template must be optimized using sample digestions to achieve the desired excision rate. Exonuclease III is not active on 3'-protruding ends of 4 bases or more in length, single-stranded DNA, or on thioester-linked nucleotides. The enzyme also has intrinsic RNase H, 3'-DNA phosphatase, and apurinic DNA endonuclease activities.
Info EPICENTRE BiotechnologiesEPICENTRE Biotechnologies
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USA
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Collected from 8-12 week-old New Zealand or Californian rabbits....
Sterile concentration of Dithiothreitol (DTT) solution in phosphate buffer (pH 7.0). Dilute contents in one vial (10- fold) to 100 ml. For use in liquefying sputum samples....
Silica bead method is an economic method of DNA extraction from agarose...
DTT, 25g...
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