|Products||Custom Protein Kinase Substrate (PKS) Peptide Microarray from LC Sciences|
|Item||Custom Protein Kinase Substrate (PKS) Peptide Microarray|
|Description|| Microarrays designed for proteomic scale kinase profiling, quantitative measurement of kinase kinetic activities, and drug discovery research built on the flexible and powerful Paraflo microfluidic on-chip synthesis platform. These microarrays are available as part of our comprehensive Protein Kinase Substrate - Peptide Microarray Service.|
Custom Probe Content
Probe content is completely customer specified. Screen your samples against thousands of possible substrate sequences for discovery, or zero in on known substrates to generate binding curves. We can easily synthesize all possible variants of your known sequences of interest or perform sequence tiling.
Traditional kinase assays focus on a single to a few peptide or protein substrates. Profiling kinases on a peptide microarray offers the opportunity to study thousands of specific substrates in a single experiment. One experiment using our PKS - peptide microarray is equivalent to 40 or more experiments using a 96-well plate.
These are not spotted arrays. The Paraflo microfluidic technology enables on-chip synthesis ensuring high probe quality, tight process control, and complete content flexibility. In addition, the microfluidic technology produces a uniform distribution of the sample solutions on the array and enhances binding reactions and stringency wash processes.
Well designed positive and negative controls as well as a universal highly phosphate-specific fluorescent dye staining reagent ensure quantitative results. There is no need for radioisotope labeling and potential signal bias due to antibody sequence specificity is eliminated. Test sequence signals can be directly compared to phosphopeptide signals (considered to be 100% of phosphorylation reading).
LC Sciences is a genomics and proteomics company offering innovative, customizable, and comprehensive microarray services for nucleic acid/protein profiling and functional analysis, biomarker-discovery, novel drug screening, and the custom development of diagnostic devices. We provide unique one-stop products and services for assays of DNA, RNA, protein, enzymes, antibodies, or small molecules, as well as proven microfluidic technology and novel microarray chemistry for design of your miniaturized assay devices for diagnostics and biosensing applications.
Our microarray services include the use of custom synthesized microarrays based on the Paraflo on-chip synthesis technology which enables the total customization of content on each individual microarray. Our customers have the flexibility to use our standard probe content or their own custom designed probe content and to use probes of their chosen lengths and number of replicates. The Paraflo technology also provides the flexibility of synthesizing microarrays containing probes with modifications such as, 5-amino linker, phosphate, biotin, and various methylation or other types of modifications. Incorporation of modifications greatly increases the number of applications for these microarrays.
LC Sciences offers additional Paraflo technology derived products that serve customers needs for construction of biomolecular libraries containing up to millions of different biomolecules of defined sequences. These products significantly reduce the cost and increase the speed of highly multiplexing genome-scale experiments such as exploration of small RNAs and designer gene/DNA library synthesis.
|Info|| LC Sciences|
2575 W. Bellfort
Houston, TX 77054
Fax Number: (713) 664-8181
Web Site: http://www.lcsciences.com