|Products||Custom Antibody Production Services from Aves Labs, Inc.|
|Company||Aves Labs, Inc.|
|Item||Custom Antibody Production Services|
|Description|| Research Scale (gram quantities of IgY)
After we receive your antigen (see How to Prepare your Antigen), we segregate the appropriate number of chickens and begin to collect pre-immune eggs. After 3 such eggs are collected (usually over a period of 3-5 days), we begin the injection series. This involves a total of four injections into the breast muscle. The first is in Complete Freunds' Adjuvant and the remaining injections use a 1:1 dilution of complete and incomplete Freund's Adjuvants. Following the fourth injection, we wait a week, and then begin to collect eggs. After 6 immune eggs are collected, we purify the IgY fraction from both pre-immune and immune eggs, and express-mail these antibodies to you.
This standard protocol typically takes 11 weeks (from the day we receive your antigen to the day you receive the purified antibody in the mail), and yields about 500 mg immune IgY and about 150 mg pre-immune IgY. The preparations we send you are filter-sterilized, and is least 90% pure IgY. Unless otherwise requested, your antibodies are in phosphate buffered isotonic saline (PBS) containing 0.02% sodium azide.
We also routinely collect an additional 12 eggs after the 6 eggs used to prepare immune antibodies, and store these eggs for a period of 2 months, in case you want us to purify additional batches of antibodies. Since egg yolk antibody titres fall relatively slowly (as compared, for example, to rabbit serum levels), the titres in the second batch of 6 eggs are likely to be the same as those in the purified immune batch.
In addition, we maintain the injected hens for 3 weeks following mailing of antibodies. If you decide to have us perform additional injections, we ask you to notify us within this 3 week period.
Large Scale (kilogram quantites of Igy)
|Info|| Aves Labs, Inc.|
Aves Labs, Inc.
12571 SW Main Street
Tigard, Oregon 97223
Customer Service: (503) 598-8766
Fax Number: (503) 598-8746
Web Site: http://www.aveslab.com