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Custom Antibody Production Services from Aves Labs, Inc.

ProductsCustom Antibody Production Services from Aves Labs, Inc.
Company Aves Labs, Inc.
Item Custom Antibody Production Services
Price 
Description Research Scale (gram quantities of IgY)
After we receive your antigen (see How to Prepare your Antigen), we segregate the appropriate number of chickens and begin to collect pre-immune eggs. After 3 such eggs are collected (usually over a period of 3-5 days), we begin the injection series. This involves a total of four injections into the breast muscle. The first is in Complete Freunds' Adjuvant and the remaining injections use a 1:1 dilution of complete and incomplete Freund's Adjuvants. Following the fourth injection, we wait a week, and then begin to collect eggs. After 6 immune eggs are collected, we purify the IgY fraction from both pre-immune and immune eggs, and express-mail these antibodies to you.

This standard protocol typically takes 11 weeks (from the day we receive your antigen to the day you receive the purified antibody in the mail), and yields about 500 mg immune IgY and about 150 mg pre-immune IgY. The preparations we send you are filter-sterilized, and is least 90% pure IgY. Unless otherwise requested, your antibodies are in phosphate buffered isotonic saline (PBS) containing 0.02% sodium azide.

We also routinely collect an additional 12 eggs after the 6 eggs used to prepare immune antibodies, and store these eggs for a period of 2 months, in case you want us to purify additional batches of antibodies. Since egg yolk antibody titres fall relatively slowly (as compared, for example, to rabbit serum levels), the titres in the second batch of 6 eggs are likely to be the same as those in the purified immune batch.

In addition, we maintain the injected hens for 3 weeks following mailing of antibodies. If you decide to have us perform additional injections, we ask you to notify us within this 3 week period.

Large Scale (kilogram quantites of Igy)
We can produce kilogram quantities of IgY (or tens of gram quantities of affinity purified antibodies) to meet your specifications and your timetable. These antibodies can be made against recombinant proteins, synthetic peptides, haptens, etc. A typical large-scale project would involve:

  • Prescreening hens with ELISAs to eliminate those that might contribute to high backgrounds.
  • Injecting hens every 3 weeks to produce hyperimmunized animals
  • Purifying the IgY fractions from eggs collected from these hens and affinity purifying the antibodies against immunosorbent materials.
  • Labeling the affinity purified antibodies with biotin, HRP, fluorescent dyes, or conjugating these antibodies to agarose.
  • Performing ELISAs and other tests to assure that the quantities, affinities, and other properties of the antibodies surpass quality control specifications.
Info Aves Labs, Inc.Aves Labs, Inc.
Aves Labs, Inc.
12571 SW Main Street
Tigard, Oregon 97223
Customer Service: (503) 598-8766
Fax Number: (503) 598-8746
Web Site: http://www.aveslab.com
NOTE: Price information is approximate list price and actual prices may vary.

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