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Price $400.00
Description Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (5). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA. The CpGenome™ DNA Modification Kit (S7820) contains the reagents necessary to perform the initial bisulfite reactions, while the CpG WIZ® DAP-kinase Amplification Kit contains the reagents required for the PCR amplification reactions. The CpG WIZ® DAP-kinase Amplification Kit contains primers that can be used for analysis of DNA samples by MSP. However, the samples must first undergo bisulfite modification prior to PCR amplification. The CpGenome™ DNA Modification Kit, S7820, contains the reagents necessary to perform the modification. Chemical modification creates the sequence differences between the methylated and unmethylated DNA. The primer sets in the kit are engineered to anneal to the DNA, based upon the sequence differences. U Primer Set will anneal to unmethylated DNA that has undergone a chemical modification. M Primer Set will anneal to methylated DNA that has undergone a chemical modification. W Primer Set serves as a control for the efficiency of chemical modification. It will anneal to any DNA (unmethylated or methylated) that has NOT undergone chemical modification, hence, the "wild type", or W. Data interpretation can still proceed in the case of incomplete chemical modification (up to 50%). The amplified region is defined as the sequence between the 3' nucleotide of the sense primer and the complement of the 3' nucleotide of the anti-sense primer for each gene promoter. The nucleotide numbering systems are those used in the GenBank submissions identified as X76104 for DAP-kinase.
CHEMICON International, Inc.
28820 Single Oak Drive
Temecula, CA 92590
Customer Service: 800-437-7500
Tech Support: 800-437-7500
Fax Number: 800-437-7502
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