Navigation Links
CaspaTag™ Pan-Caspase In Situ Assay Kit, Sulforhodamine from CHEMICON

ProductsCaspaTag™ Pan-Caspase In Situ Assay Kit, Sulforhodamine from CHEMICON
Company CHEMICON
Item CaspaTag™ Pan-Caspase In Situ Assay Kit, Sulforhodamine
Price $193.00
Description The CHEMICON® CaspaTag™ Pan-Caspase In Situ Assay Kit is a fluorescent-based assay for detection of active caspases in cells undergoing apoptosis. The kit is for research use only. Not for use in diagnostic or therapeutic procedures. CHEMICON®s CaspaTag™ Pan-Caspase In Situ Assay Kits use a novel approach to detect active caspases. The methodology is based on Fluorochrome Inhibitors of Caspases (FLICA). The inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the inhibitor binds covalently to the active caspase7. This kit uses a sulforhodamine-labeled fluoromethyl ketone peptide inhibitor of caspase (SR-VAD-FMK), which produces a red fluorescence. When added to a population of cells, the SR-VAD-FMK probe enters each cell and covalently binds to a reactive cysteine residue that resides on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. The bound labeled reagent is retained within the cell, while any unbound reagent will diffuse out of the cell and is washed away. The red fluorescent signal is a direct measure of the amount of active caspase present in the cell at the time the reagent was added. Cells that contain the bound labeled reagent can be analyzed by 96-well plate-based fluorometry, fluorescence microscopy, or flow cytometry.

KIT COMPONENTS:
  1. CaspaTag™ Reagent (SR-VAD-FMK): One lyophilized vial
  2. 10X Wash Buffer: 15 mL
  3. Fixative: 6 mL
  4. Hoechst Stain: 1 mL at 200 Μg/mL, ready-to-use
Info CHEMICONCHEMICON
CHEMICON International, Inc.
28820 Single Oak Drive
Temecula, CA 92590
Customer Service: 800-437-7500
Tech Support: 800-437-7500
Fax Number: 800-437-7502
Web Site: http://www.chemicon.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. CaspaTag™ Pan-Caspase In Situ Assay Kit, Fluorescein from CHEMICON
2. In Situ Pan-Caspase Assay Kit, Sulforhodamine from CHEMICON
3. In Situ Pan-Caspase Assay Kit from CHEMICON
4. Glutathione-S-Transferase (GST) Colorimetric Assay Kit from Sigma-Aldrich
5. In Vitro Osteogenesis Assay Kit from CHEMICON
6. Mast Cell Degranulation colorimetric Assay Kit from CHEMICON
7. PR Competitor Assay Green from Invitrogen
8. PolarScreen Androgen Receptor Competitor Assay Kit, Red from Molecular Probes (Invitrogen)
9. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
10. PPARGamma-Competitor Assay Kit Green from Invitrogen
11. Total antioxidant capacity Colorimetric Assay Kit from Neogen Corporation
... Use in any standard fixed-angle microcentrifuge ... g.,Spin- centrifuge tube filters consist ... a centrifuge tube. They filter by ... HPLC sample preparation, removal of cells ...
... Simplify the process and you reduce ... built into the CEQ 8000. Developed using ... and leadership in capillary electrophoresis technology, the ... analysis system. This system automatically fills the ...
... a convenient method to probe conformational changes in ... Thus, the kit may be used to determine ... transport substrates for MDR1. Because UIC2 binding is ... the MDR1 Shift Assay can be utilized for ...
... The Chemicon Detergent-OUT SDS Kit is ... detergents from protein solutions. Removes detergents ... Simply load the protein solution on the ... protein solution. Med columns are suitable ...
Biology Products:
(Date:1/6/2017)... BOULDER, Colo. , Jan. 5, 2017 /PRNewswire/ ... join the "Digital Life Alliance" established by iCarbonX, ... in 2015 to build a "Global Digital Health ... based on a combination of individual,s biological, behavioral ... Under the agreement between the companies, SomaLogic will ...
(Date:1/4/2017)... of attendees at this year,s International Consumer Electronics Show (CES), A&D ... and services, will be featuring its new line of ULTRA CONNECT ... CES Exhibit Suite , the new upper arm and wrist smart blood ... product platform.  Continue Reading ... ...
(Date:1/3/2017)... -- Onitor, provider of digital health technology for consumers, ... biometric data-driven program designed to aid weight loss for ... Consumer Electronics Show (CES) in Las Vegas ... World Health Organization (WHO), have identified lifestyle risks that ... overweight or obese. WHO also states that more people ...
Breaking Biology News(10 mins):
(Date:1/12/2017)... ... January 12, 2017 , ... ... 26-year-old Lisa Rosendahl’s doctors gave her only a few months to live. Now ... combination that has stabilized Rosendahl’s disease and increased both the quantity and quality ...
(Date:1/12/2017)... January 12, 2017 The report "Direct-Fed Microbials Market by Type (Lactic ... and Liquid), and Region - Global Forecast to 2022", published by MarketsandMarkets, the ... and projected to reach USD 1,399.6 Million by 2022, at a CAGR of ... ... MarketsandMarkets Logo ...
(Date:1/11/2017)... ... January 11, 2017 , ... ... clinics in Peru studying the pathogens that cause malaria and tuberculosis. Seeing firsthand ... path of discovery. , Now, as an assistant professor of biology and biotechnology ...
(Date:1/11/2017)... Linda, Ca (PRWEB) , ... January 11, 2017 ... ... resistance in pathogens are observed in clinical settings, it is becoming increasingly clear ... problem. An over-reliance on culture-based methods, the standard in the study of clinical ...
Breaking Biology Technology: