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CaspaTag™ Pan-Caspase In Situ Assay Kit, Fluorescein from CHEMICON

ProductsCaspaTag™ Pan-Caspase In Situ Assay Kit, Fluorescein from CHEMICON
Company CHEMICON
Item CaspaTag™ Pan-Caspase In Situ Assay Kit, Fluorescein
Price $162.00
Description The CHEMICON® CaspaTag™ Caspase-9 Pan-Caspase In Situ Assay Kit, Fluorescein is a fluorescent-based assay for detection of active caspases in cells undergoing apoptosis. The kit is for research use only. Not for use in diagnostic or therapeutic procedures. CHEMICON®s CaspaTag™ In Situ Caspase Detection Kits use a novel approach to detect active caspases. The methodology is based on Fluorochrome Inhibitors of Caspases (FLICA). The inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the inhibitor binds covalently to the active caspase7. This kit uses a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of caspase (FAM-VAD-FMK), which produces a green fluorescence. When added to a population of cells, the FAM-VAD-FMK probe enters each cell and covalently binds to a reactive cysteine residue that resides on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. The bound labeled reagent is retained within the cell, while any unbound reagent will diffuse out of the cell and is washed away. The green fluorescent signal is a direct measure of the amount of active caspase present in the cell at the time the reagent was added. Cells that contain the bound labeled reagent can be analyzed by 96-well plate-based fluorometry, fluorescence microscopy, or flow cytometry.

KIT COMPONENTS:
  1. FLICA Reagent (FAM-LETD-FMK): One lyophilized vial
  2. 10X Wash Buffer: 15 mL
  3. Fixative: 6 mL
  4. Propidium Iodide: 1 mL at 250 Μg/mL, ready-to-use
  5. Hoechst Stain: 1 mL at 200 Μg/mL, ready-to-use
Info CHEMICONCHEMICON
CHEMICON International, Inc.
28820 Single Oak Drive
Temecula, CA 92590
Customer Service: 800-437-7500
Tech Support: 800-437-7500
Fax Number: 800-437-7502
Web Site: http://www.chemicon.com
NOTE: Price information is approximate list price and actual prices may vary.

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