Navigation Links
CaspaTag™ Pan-Caspase In Situ Assay Kit, Fluorescein from CHEMICON

ProductsCaspaTag™ Pan-Caspase In Situ Assay Kit, Fluorescein from CHEMICON
Company CHEMICON
Item CaspaTag™ Pan-Caspase In Situ Assay Kit, Fluorescein
Price $162.00
Description The CHEMICON® CaspaTag™ Caspase-9 Pan-Caspase In Situ Assay Kit, Fluorescein is a fluorescent-based assay for detection of active caspases in cells undergoing apoptosis. The kit is for research use only. Not for use in diagnostic or therapeutic procedures. CHEMICON®s CaspaTag™ In Situ Caspase Detection Kits use a novel approach to detect active caspases. The methodology is based on Fluorochrome Inhibitors of Caspases (FLICA). The inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the inhibitor binds covalently to the active caspase7. This kit uses a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of caspase (FAM-VAD-FMK), which produces a green fluorescence. When added to a population of cells, the FAM-VAD-FMK probe enters each cell and covalently binds to a reactive cysteine residue that resides on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. The bound labeled reagent is retained within the cell, while any unbound reagent will diffuse out of the cell and is washed away. The green fluorescent signal is a direct measure of the amount of active caspase present in the cell at the time the reagent was added. Cells that contain the bound labeled reagent can be analyzed by 96-well plate-based fluorometry, fluorescence microscopy, or flow cytometry.

KIT COMPONENTS:
  1. FLICA Reagent (FAM-LETD-FMK): One lyophilized vial
  2. 10X Wash Buffer: 15 mL
  3. Fixative: 6 mL
  4. Propidium Iodide: 1 mL at 250 Μg/mL, ready-to-use
  5. Hoechst Stain: 1 mL at 200 Μg/mL, ready-to-use
Info CHEMICONCHEMICON
CHEMICON International, Inc.
28820 Single Oak Drive
Temecula, CA 92590
Customer Service: 800-437-7500
Tech Support: 800-437-7500
Fax Number: 800-437-7502
Web Site: http://www.chemicon.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. CaspaTag™ Pan-Caspase In Situ Assay Kit, Sulforhodamine from CHEMICON
2. In Situ Pan-Caspase Assay Kit, Sulforhodamine from CHEMICON
3. In Situ Pan-Caspase Assay Kit from CHEMICON
4. Glutathione-S-Transferase (GST) Colorimetric Assay Kit from Sigma-Aldrich
5. In Vitro Osteogenesis Assay Kit from CHEMICON
6. Mast Cell Degranulation colorimetric Assay Kit from CHEMICON
7. PR Competitor Assay Green from Invitrogen
8. PolarScreen Androgen Receptor Competitor Assay Kit, Red from Molecular Probes (Invitrogen)
9. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
10. PPARGamma-Competitor Assay Kit Green from Invitrogen
11. Total antioxidant capacity Colorimetric Assay Kit from Neogen Corporation
... assay reagents package is used for determining protein ... detergents. The RC DC protein assay is a ... 500 standard assays: 250 ml RC reagent I, ... copper tartrate solution, 2 L dilute Folin reagent, ...
... provides a powerful yet simple technology for ... The kit provides the heterobifunctional linkers SFB ... conjugation efficiencies. It enables conjugation of various ... tags with a variety of proteins. ...
...
MAb to HLA, Class II-DR+DP Dry Ice: No Preservative: NaN3 Availability When Not in Inventory: 14-21 Business Days...
Biology Products:
(Date:3/15/2016)... , March 15, 2016 --> ... published by Transparency Market Research "Digital Door Lock Systems Market ... 2015 - 2023," the global digital door lock systems market ... in 2014 and is forecast to grow at a CAGR ... micro, small and medium enterprises (MSMEs) across the world and ...
(Date:3/11/2016)... 2016 --> ... "Image Recognition Market by Technology (Pattern Recognition), by Component ... Deployment Type (On-Premises and Cloud), by Industry Vertical and ... MarketsandMarkets, the global market is expected to grow from ... by 2020, at a CAGR of 19.1%. ...
(Date:3/9/2016)... Fla. , March 9, 2016  Crossmatch ... authentication and enrollment solutions, today announced the addition ... ® Altus multi-factor authentication platform. New ... InfoSec managers to step-up security where it,s needed ... Washington, DC . --> ...
Breaking Biology News(10 mins):
(Date:5/25/2016)... New Haven, Connecticut (PRWEB) , ... May 25, ... ... today that the U.S. Food and Drug Administration (FDA) has granted the company’s ... This is the company’s second orphan drug designation granted by the FDA. ...
(Date:5/24/2016)... , May 24, 2016   MedyMatch Technology Ltd ... with artificial intelligence, real-time decision support tools in the emergency ... at the 2016 Israeli Advanced Technology Industries (IATI) BioMed Conference. ... Israel,s 15th National Life Sciences and Technology ... the David Intercontinental Hotel in Tel Aviv, Israel ...
(Date:5/24/2016)... ... May 24, 2016 , ... Media Cybernetics, global image analysis ... corporate branding reflects a results-driven revitalization for a company with a renewed focus ... include a crisp, refreshed logo and a new web presence. , “I believe ...
(Date:5/23/2016)... ... May 23, 2016 , ... PrecisionAg® Media has released its ... Beyond. The paper outlines the key trends that are creating both opportunities and ... a lot of highs and lows as the precision agriculture market has grown ...
Breaking Biology Technology: