Navigation Links
Canine Pancreatic Microsomal Membranes from Promega

ProductsCanine Pancreatic Microsomal Membranes from Promega
Company Promega
Item Canine Pancreatic Microsomal Membranes
Price Inquire
Description Microsomal vesicles are used to study co-translational and initial post-translational processing of proteins. Processing events such as signal peptide cleavage, membrane insertion, translocation and core glycosylation can be examined by the translation of the appropriate mRNA in vitro in the presence of these microsomal membranes. In addition, processing and glycosylation events may be studied by the transcription/translation of the appropriate DNA in the TNT® Lysate Systems when used with Canine Pancreatic Microsomal Membranes(a). To assure consistent performance with minimal translational inhibition and background, microsomes have been isolated free from contaminating membrane fractions and stripped of endogenous membrane-bound ribosomes and mRNA (1). Membrane preparations are assayed for both signal peptidase and core glycosylation activities using two different control mRNAs. The two control mRNAs supplied with this system are the precursor for β-lactamase (or ampicillin resistance gene product) from E. coli and the precursor for α-mating factor (or α-factor gene product) from S. cerevisiae. • The Signal Sequence Control mRNA (E. coli β-lactamase) is transcribed by SP6 RNA polymerase from a plasmid bearing the coding region for the E. coli gene encoding the precursor to β-lactamase (the ampicillin resistance gene product). The RNA is synthesized without a cap analog. This control mRNA is used to assay for signal peptidase activity and is supplied with the Canine Pancreatic Microsomal Membranes System. • The Core Glycosylation Control mRNA (S. cerevisiae α-factor) is transcribed by SP6 RNA polymerase from a plasmid bearing the coding region for the S. cerevisiae α-mating factor. The RNA is synthesized without a cap analog. This control mRNA is used to assay for core glycosylation activity and is supplied with the Canine Pancreatic Microsomal Membranes System.

Features:
Microsomes are stripped of endogenous membrane-bound ribosomes and mRNA to ensure consistent performance with minimal translational inhibition and background. Performance tested in rabbit reticulocyte lysate.

Applications:
Examination of signal peptide cleavage, membrane insertion, translocation and core glycosylation. • Compatible with the TNT® Lysate Systems, Rabbit Reticulocyte Lysate, and Flexi® Lysate.
Info PromegaPromega
2800 Woods Hollow Rd.
Madison, WI 53711-5399 USA
Customer Service: 800-356-9526
Tech Support: 1-800-356-9526
Fax Number: 800-356-1970
Web Site: http://www.promega.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Canine Endostatin EIA Kit from Neogen Corporation
2. Canine serum from Equitech-Bio
3. Normal Canine Serum - 100mL from Biomeda Corporation
4. Canine CCL2 (JE; MCP-1) ELISA development Kit, DuoSet from R&D Systems
5. Canine IL-10 ELISpot Kit from R&D Systems
6. Canine IL-4 ELISpot Kit from R&D Systems
7. Canine IL-10 ELISpot Development Kit from R&D Systems
8. Canine IL-4 ELISpot Development Kit from R&D Systems
9. Canine IFN-gamma ELISpot Development Kit from R&D Systems
10. Canine IFN-gamma ELISA Kit from R&D Systems
11. Canine Serum from CHEMICON
Synaptic Vesicle I Sampler Kit 10g each...
Hybridization chamblers suitable for use with all ChIP-GLAS microarrays....
Rabbit polyclonal to BCA1 ( Abpromise for all tested applications). Antigen: Generated using E.coli expressed mouse BCA1. Entrez GeneID: 10563 SWISS Protein ID: O43927...
SH3 Proteins II Sampler Kit 10g each...
Biology Products:
(Date:3/2/2017)... Australian stem cell and regenerative medicine company, ... an agreement with the Monash Lung Biology Network, a ... and Department of Pharmacology at Monash University, ... to support the use of Cymerus™ mesenchymal stem cells ... Asthma is a chronic, long term lung condition recognised ...
(Date:2/27/2017)... WASHINGTON , Feb. 27, 2017   Strategic ... fund, today announced it has led a $3.5 million investment ... collaboration platform. Strategic Cyber Ventures is DC based and ... and Hank Thomas . Ron Gula , ... Tech Ventures, also participated in this series A round ...
(Date:2/21/2017)... , February 21, 2017 Der ... US-Dollar wachsen. Nach einem Gespräch mit mehr als 50 Vertretern ... Hindernisse zu überwinden gilt, um diese Prognose zu realisieren. ... ... die Mobilisierung der finanziellen Mittel für die Biobank, die ...
Breaking Biology News(10 mins):
(Date:3/29/2017)... , March 29, 2017 QIAGEN N.V. (NASDAQ: ... today announced the U.S. launch of its ipsogen ® JAK2 ... U.S. Food and Drug Administration as a qualitative in vitro diagnostic test for ... whole blood.* ... assay is processed on QIAGEN,s Rotor-Gene ® Q MDx system, which is ...
(Date:3/29/2017)... March 29, 2017 /PRNewswire/ - Last year,s petition to the ... the only efforts active to generate awareness on the importance of ... drugs in the healthcare market place. ... Earlier this month Health Advance ... of pharmaceutical and nutritional products, announced its endeavors on this ...
(Date:3/29/2017)... PETACH TIKVAH, Israel , ... Inc. (NASDAQ: BCLI), a leading developer of ... announced financial results for the year ended ... a highly successful and pivotal year for Brainstorm, with ... progress made on clinical, regulatory and operational fronts," ...
(Date:3/29/2017)... ... March 29, 2017 , ... ComplianceOnline, the leading governance, ... announced its 3rd Annual Medical Device Summit 2017 venue and speaker lineup. The Summit ... Hotel, in Boston, MA. , The Omni Parker House Hotel, which is located at ...
Breaking Biology Technology: