Navigation Links
Canine Pancreatic Microsomal Membranes from Promega

ProductsCanine Pancreatic Microsomal Membranes from Promega
Company Promega
Item Canine Pancreatic Microsomal Membranes
Price Inquire
Description Microsomal vesicles are used to study co-translational and initial post-translational processing of proteins. Processing events such as signal peptide cleavage, membrane insertion, translocation and core glycosylation can be examined by the translation of the appropriate mRNA in vitro in the presence of these microsomal membranes. In addition, processing and glycosylation events may be studied by the transcription/translation of the appropriate DNA in the TNT® Lysate Systems when used with Canine Pancreatic Microsomal Membranes(a). To assure consistent performance with minimal translational inhibition and background, microsomes have been isolated free from contaminating membrane fractions and stripped of endogenous membrane-bound ribosomes and mRNA (1). Membrane preparations are assayed for both signal peptidase and core glycosylation activities using two different control mRNAs. The two control mRNAs supplied with this system are the precursor for β-lactamase (or ampicillin resistance gene product) from E. coli and the precursor for α-mating factor (or α-factor gene product) from S. cerevisiae. • The Signal Sequence Control mRNA (E. coli β-lactamase) is transcribed by SP6 RNA polymerase from a plasmid bearing the coding region for the E. coli gene encoding the precursor to β-lactamase (the ampicillin resistance gene product). The RNA is synthesized without a cap analog. This control mRNA is used to assay for signal peptidase activity and is supplied with the Canine Pancreatic Microsomal Membranes System. • The Core Glycosylation Control mRNA (S. cerevisiae α-factor) is transcribed by SP6 RNA polymerase from a plasmid bearing the coding region for the S. cerevisiae α-mating factor. The RNA is synthesized without a cap analog. This control mRNA is used to assay for core glycosylation activity and is supplied with the Canine Pancreatic Microsomal Membranes System.

Features:
Microsomes are stripped of endogenous membrane-bound ribosomes and mRNA to ensure consistent performance with minimal translational inhibition and background. Performance tested in rabbit reticulocyte lysate.

Applications:
Examination of signal peptide cleavage, membrane insertion, translocation and core glycosylation. • Compatible with the TNT® Lysate Systems, Rabbit Reticulocyte Lysate, and Flexi® Lysate.
Info PromegaPromega
2800 Woods Hollow Rd.
Madison, WI 53711-5399 USA
Customer Service: 800-356-9526
Tech Support: 1-800-356-9526
Fax Number: 800-356-1970
Web Site: http://www.promega.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Canine Endostatin EIA Kit from Neogen Corporation
2. Canine serum from Equitech-Bio
3. Normal Canine Serum - 100mL from Biomeda Corporation
4. Canine CCL2 (JE; MCP-1) ELISA development Kit, DuoSet from R&D Systems
5. Canine IL-10 ELISpot Kit from R&D Systems
6. Canine IL-4 ELISpot Kit from R&D Systems
7. Canine IL-10 ELISpot Development Kit from R&D Systems
8. Canine IL-4 ELISpot Development Kit from R&D Systems
9. Canine IFN-gamma ELISpot Development Kit from R&D Systems
10. Canine IFN-gamma ELISA Kit from R&D Systems
11. Canine Serum from CHEMICON
Rabbit polyclonal to SERHL ( Abpromise for all tested applications). entrezGeneID: 94009 SwissProtID: Q9H4I8...
MDMA Mouse Monoclonal Antibody (Clone 3G8)...
Mouse anti-hepatitis delta virus Class: Antibody Product Group: Bacterial and Viral Antigen...
Mouse monoclonal [MN-8C4] to zona radiata ( Abpromise for all tested applications). Antigen: Purified eggshell proteins (zona radiata proteins and small amounts of zona pellucida proteins) from ...
Biology Products:
(Date:4/13/2016)... 2016  IMPOWER physicians supporting Medicaid patients in ... clinical standard in telehealth thanks to a new partnership ... platform, IMPOWER patients can routinely track key health measurements, ... index, and, when they opt in, share them with ... a local retail location at no cost. By leveraging ...
(Date:3/29/2016)... 2016 LegacyXChange, Inc. (OTC: ... SelectaDNA/CSI Protect are pleased to announce our successful effort ... variety of writing instruments, ensuring athletes signatures against counterfeiting ... from athletes on LegacyXChange will be assured of ongoing ... Bill Bollander , CEO states, "By ...
(Date:3/22/2016)... PROVO and SANDY, Utah ... Ontario (NSO), which operates the highest sample volume laboratory ... and Tute Genomics and UNIConnect, leaders in clinical sequencing ... announced the launch of a project to establish the ... panel. NSO has been contracted by ...
Breaking Biology News(10 mins):
(Date:6/27/2016)...  Liquid Biotech USA , ... Sponsored Research Agreement with The University of Pennsylvania ... cancer patients.  The funding will be used to ... clinical outcomes in cancer patients undergoing a variety ... employed to support the design of a therapeutic, ...
(Date:6/24/2016)... ... June 24, 2016 , ... While the majority of commercial spectrophotometers and fluorometers ... the 6000i models are higher end machines that use the more unconventional z-dimension of ... beam from the bottom of the cuvette holder. , FireflySci has developed several ...
(Date:6/23/2016)... 23, 2016   Boston Biomedical , an ... designed to target cancer stemness pathways, announced that ... Orphan Drug Designation from the U.S. Food and ... cancer, including gastroesophageal junction (GEJ) cancer. Napabucasin is ... inhibit cancer stemness pathways by targeting STAT3, and ...
(Date:6/23/2016)... ... 2016 , ... Charm Sciences, Inc. is pleased to announce ... Research Institute approval 061601. , “This is another AOAC-RI approval of the Peel ... President of Regulatory and Industrial Affairs. “The Peel Plate methods perform comparably to ...
Breaking Biology Technology: