Navigation Links
Calf Topoisomerase I from USB Corp.

ProductsCalf Topoisomerase I from USB Corp.
Company USB Corp.
Item Calf Topoisomerase I
Price 
Description Source: Calf thymus
Description:
Topoisomerase I relaxes both positively and negatively supercoiled DNA by a transient breakage and rejoining of phosphodiester bonds(1,2). Relaxing supercoiled DNA with Topoisomerase I allows molecules to be differentiated by a single nucleotide pair by gel electrophoresis(3). Topoisomerase I is active in the absence of Mn2+ and can be used in solutions containing EDTA.
Purity:
Tested for contaminating endonucleases and exonucleases.
Storage Buffer:
20mM potassium phosphate (pH 7.2), 50mM KCl, 0.05mM EDTA, 5mM 2-mercaptoethanol, 0.02% BSA, 50% glycerol.
Shipping and Storage:
Shipped on dry ice. Store at -20C.
Unit Definition:
One unit is the amount of enzyme required to catalyze the conversion of 0.5 g supercoiled pBR322 DNA to a relaxed state in 30 min at 37C.
Concentration:5 units/l
Applications:*Analysis of DNA supercoiling and conformation(2) .
*Produces knots in single-stranded, circular DNA(2).
*Analysis of chromatin reconstitution in vitro(4).
*DNA repair studies(4).
*Studies on drug resistance, cell proliferation and leukemia(5,6).
Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128


Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-216-245-1829
Customer Service: 216.765.5000
Fax Number: 216.464.5075
Web Site: http://www.usbweb.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Topoisomerase I from Invitrogen
2. Vaccinia DNA Topoisomerase I from EPICENTRE Biotechnologies
3. Vaccinia DNA Topoisomerase I from EPICENTRE Biotechnologies
4. Topoisomerase I from Invitrogen
5. Human Topoisomerase I Wild Type from Calbiochem
6. Human Topoisomerase II, Alpha from USB Corp.
7. Human Topoisomerase II, Alpha from USB Corp.
8. Anti-Topoisomerase II (Ki-S1) Monoclonal Antibody, Unconjugated, Clone P-tyr-I from CHEMICON
9. Topoisomerase I (Vaccinia) from Molecular Cloning Laboratories (MCLAB)
10. Topoisomerase I from Promega
11. S-Acetyl Coenzyme A Lithium Salt from USB Corp.
Viraffinity 100 from Biotech Support Group
Tumor Suppressor Sampler Kit from BD Biosciences Pharmingen
Mouse Anti-Synapsin I Monoclonal Antibody, Unconjugated, Clone 223 from IMGENEX
Slide Chambers from Novagen
Biology Products:
(Date:11/23/2009)...dy provides "incontrovertible evidence" that the v...matra about 73,000 years ago deforested much of ce...researchers report. , The volcano ejected an est...here, leaving a crater (now the world,s largest vo...lometers wide. Ash from the event has been found i...
(Date:11/23/2009)...09 -- A USDOE and USDA study concluded that 50 mil...opland pasture could be converted from current use...switchgrass, from which biomass could be harvested...le production of a perennial grass monoculture fro...ved annually is expected to require nitrogen ferti...
(Date:11/23/2009)...dies reveal in unprecedented detail how the riboso...proteins and guide them toward their destination i...amics flexible fitting (MDFF) to examine the inter...r partners. , The first study, in Proceedings ... intimate signaling between the ribosome and an el...
Breaking Biology News(10 mins):Supervolcano eruption -- in Sumatra -- deforested India 73,000 years ago 2Switchgrass produces biomass efficiently 2Computational microscope peers into the working ribosome 2Computational microscope peers into the working ribosome 3Schofield Media Names Brian Reshefsky Group President 56972 1Coast Dental Deploys Demandforce D3 Communications Platform 56970 1Coast Dental Deploys Demandforce D3 Communications Platform 56970 2Discovery May Pave Way to Better Diabetes Care 56967 1
...on, WI -- 12 AUGUST 2008 -- More than 10,000 inter... students will gather at the George R. Brown Conve...cuss the latest research and trends in energy, wat... education, earth sciences, and related discipline...llaboration that showcases the sciences of the spo...
...ng ways of controlling a devastating new wheat dis...duction and food supply crisis., Cornell Universit... & Melinda Gates Foundation Global Development Pro...earch as part of the Durable Rust Resistance in Wh..., wheat stem rust. , World leaders in rust resear...
... University of California, Davis, law professor Ke...property protection for plants over the past sever...re that plant genetic resources remain freely avai...eeders and researchers. , "Seed Wars: Controversi...ectual Property" provides a comprehensive overview...
Other Biology News:Scientists team up in Houston to tackle global challenges 2Scientists team up in Houston to tackle global challenges 3Scientists team up in Houston to tackle global challenges 4CSIRO enlisted to avert global wheat supply crisis 2
(Date:11/24/2009)..., TORONTO,Nov.24/PRNewswire-FirstCall/-Hamilt...,aleadingproviderofadvancedlasersystemsandinstrume...,todayreportedoperationalandfinancialresultsforthe...ights,-Revenuewas$1.46millioncomparedwith$1.43mil...redwith66.1percentinQ3,2008,-Netlossreducedto$148,...
(Date:11/24/2009)... Program Connects Customers with Partners Valida...DNA Genotek Products , Ot... , a leading provider of products for biological s...oday announced its new global partner program. The...enotek,s partner community and is designed to help...
(Date:11/23/2009)...mises ultra-fast communication, computation and mo... But trying to use quantum states as carriers of i...two physicists have shown, mathematically, how to ...cation, while keeping the information intact. Thei...al Review Letters and highlighted with a Viewpoin...
(Date:11/23/2009)... Contec’s Sporicidin disinfectant solution...de disinfectants against H1N1 and Influenza A., ,...ovember 24, 2009 -- Contec Inc, the leading manufa...t their Sporicidin disinfectants have been deeme...viruses, including Pandemic 2009 H1N1 influenza A ...
Breaking Biology Technology:Hamilton Thorne announces third quarter results 2Hamilton Thorne announces third quarter results 3Hamilton Thorne announces third quarter results 4Hamilton Thorne announces third quarter results 5Hamilton Thorne announces third quarter results 6Hamilton Thorne announces third quarter results 7DNA Genotek Launches Global Partner Program 2DNA Genotek Launches Global Partner Program 3Sporicidin Brand Disinfectant Kills H1N1 in 3 Minutes 2