|Products||BlockAid blocking solution *for use with microspheres* from Molecular Probes (Invitrogen)|
|Company||Molecular Probes (Invitrogen)|
|Item||BlockAid blocking solution *for use with microspheres*|
|Description|| Molecular Probes intensely fluorescent and highly photostable FluoSpheres microspheres have enormous potential for use in a variety of applications requiring probes that can deliver a strong signal. Unfortunately, protein- and other macromolecule-labeled microspheres have hydrophobic regions that may cause them to bind to non-target surfaces in some applications. This nonspecific binding can be relieved by the use of a blocking solution; however, we have found that microspheres require a stronger blocking solution than those in common use.
BlockAid blocking solution is designed for use with our streptavidin-, NeutrAvidin-, biotin- and protein Alabeled FluoSpheres microspheres. However, we have also found it to be useful in some cases for reducing nonspecific binding by dyelabeled antibodies. In our tests, the protein-based BlockAid blocking solution was mixed with streptavidin-labeled Fluo- Spheres microspheres, which were then used to stain several different cell types for flow cytometry. We found that BlockAid blocking solution was superior to blocking solutions available from other companies as well as home-made blocking solutions described in the scientific literature for reducing nonspecific binding of labeled microspheres. BlockAid blocking solution has been found to be effective in flow cytometry applications involving NIH 3T3, A431, RAW and JURKAT cell lines, although less effective in HMC-1 cells. We expect BlockAid blocking solution to be useful for reducing the nonspecific binding of protein-coated or other macromolecule-coated microspheres in a variety of flow cytometry and microscopy applications.
|Info|| Molecular Probes (Invitrogen)|
PO Box 22010
Eugene, OR 97402-0469
Fax Number: 1-541-344-6504
Web Site: http://probes.invitrogen.com