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Apoptotic Cell Isolation Kit from MBL International

ProductsApoptotic Cell Isolation Kit from MBL International
Company MBL International
Item Apoptotic Cell Isolation Kit
Price 
Description 30 isolations. The kit provides a simple and efficient means for isolation of apoptotic cells from normal c lture using annexin V magnetic beads (MagBeads). Annexin V is a Ca2+-dependent phospholipid binding protein with high affinity for phosphatidylseri

The Apoptotic Cell Isolation Kit provides a simple and efficient means for isolation of apoptotic cells or removal of dead cells from cell culture or tissue preparations using annexin V/magnetic beads (MagBeads). Annexin V is a Ca2+-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), which is redistributed from the inner to the outer plasma membrane leaflet in apoptotic or dead cells. Once on the cell surface, PS becomes available for binding to annexin V and any of its conjugates. Binding of annexin V-biotin to apoptotic cells followed by binding of the biotin to streptavidin-MagBeads enables separation of apoptotic cells from living cells. The apoptotic cells bound to the MagBeads adhere to the magnet, while nonapoptotic cells stay in suspension. The separated apoptotic and healthy cells can then be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from healthy cell cultures.
Info MBL InternationalMBL International
15 B Constitution Way
Woburn, MA 01801
Customer Service: 781-939-6964
Fax Number: 781-939-6963
Web Site: http://mblintl.com
NOTE: Price information is approximate list price and actual prices may vary.

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... designed for use with the ProQuest Two-Hybrid ... contains deletions in the endogenous GAL4 and ... systems. The strain also has leu2 and ... and prey vectors. MaV203 contains three GAL4 ...
... MaV203 Competent Yeast Cells are designed for ... 1). S. cerevisiae strain MaV203 contains deletions ... for use with GAL4-based two-hybrid systems. The ... for selection of ProQuest bait and prey ...
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... protein in 200 l SDS-PAGE Western blotting buffer, ... transformed macrophage cells, whole cell ... should be stored at -20C and ... sample vial should be boiled once ...
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