Navigation Links
Apo-Direct Apoptosis Detection Kit from eBioscience

ProductsApo-Direct Apoptosis Detection Kit from eBioscience
Company eBioscience
Item Apo-Direct Apoptosis Detection Kit
Price $395.00
Description The APO-DIRECT Kit is a 2-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl transferase enzyme (TdT), Fluorescein-deoxyuridine triphosphate and propidium iodide/RNase A solution for counterstaining the total DNA.

One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. These DNA fragments can be extracted from apoptotic cells and result in the appearance of DNA laddering when the DNA is analyzed by agarose gel electrophoresis. The DNA of non-apoptotic cells that remains largely intact does not display this laddering on agarose gels during electrophoresis. The large number of DNA fragments appearing in apoptotic cells results in a multitude of 3'-hydroxyl termini in the DNA. This property can be used to identify apoptotic cells by labeling the 3'-hydroxyl ends with directly conjugated fluorescein- deoxyuridine triphosphate nucleotides (FITC-dUTP). The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes a template-independent addition of deoxyribonucleoside triphosphates to the 3'-hydroxyl ends of double- or single-stranded DNA with either blunt, recessed or overhanging ends. A substantial number of these sites are available in apoptotic cells providing the basis for the method utilized in the APO-DIRECT Kit. Non-apoptotic cells do not incorporate significant amounts of the FITC-dUTP due to the lack of exposed 3'-hydroxyl DNA ends.
Info eBioscienceeBioscience
6042 Cornerstone Court West
San Diego, CA 92121
Customer Service: (888) 999-1371
Fax Number: (858) 642-2046
Web Site: http://www.ebioscience.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit from CHEMICON
2. ApopTag Fluorescein In Situ Apoptosis Detection Kit from CHEMICON
3. ApopTag Red In Situ Apoptosis Detection Kit from CHEMICON
4. Apo-BrdU Apoptosis Detection Kit from eBioscience
5. MitoLight Mitochondrial Apoptosis Detection Kit from CHEMICON
6. MitoLightApoptosis Detection Kit from CHEMICON
7. Apoptosis Pathway SpliceArrays from ExonHit Therapeutics Inc.
8. Flow Cytometry Kit for Apoptosis from Sigma-Aldrich
9. Human Apoptosis Kit 50 tests from BD Biosciences Pharmingen
10. Vybrant Apoptosis Assay Kit #4 from Molecular Probes (Invitrogen)
11. MitoCapture Apoptosis Detection Kit from BioVision
Automated Cell Culture Flask...
... high-throughput fluidics (HTF) system, 100-240 V, is ... array system that automates delivery of up ... the Bio-Plex array reader. The HTF system ... bulk sheath container to constantly maintain a ...
... laboratory, the revolutionary Luminex 100 System delivers ... in real time enhancing your operational efficiency. ... the Luminex 100, the XY Platform, and ... enables you to simultaneously assay up to ...
... This gradient system is ideal for routine binary ... highly sensitive Adept CE 4201 UV-Visible variable wavelength ... is provided by the two highly accurate ... CE 4100 solvent delivery pumps., Chromatograms may be ...
Biology Products:
(Date:8/28/2014)... is now available from the Lady Bird Johnson Wildflower ... identifying harmful non-native plant, insect and other invasive species ... cause about $137 billion in economic and other damage ... native plants and more. , "By having streamlined tools ... species occur, we,re more likely to catch these threats ...
(Date:8/28/2014)... your neighbors than traditional cigarettes, but still release ... study from USC. , Scientists studying secondhand smoke ... exposure to harmful particles, with close-to-zero exposure to ... harmful metals in second-hand e-cigarette smoke were found ... contains high levels of polycyclic aromatic hydrocarbons ...
(Date:8/28/2014)... Ga. The support of peer groups and ... breastfeeding programs, according to recent University of Georgia ... in the Athens-Clarke County area determined that role ... outcomes of mothers of infants. , "Mothers who ... successful at breastfeeding," said study co-author Alex Anderson, ...
Breaking Biology News(10 mins):Mobile app makes ID of harmful plants, insects in Texas a snap 2Second-hand e-cig smoke compared to regular cigarette smoke 2Breastfeeding study shows need for effective peer counseling programs 2
... For clinicians, the ability to look routinely inside the body ... how it confronts disease is a distant dream. But in ... of researchers from the Howard Hughes Medical Institute (HHMI) at ... step toward realizing that vision by demonstrating the ability to ...
... insect forms—beetles, flies, dragonflies, mosquitoes, grasshoppers, ants, wasps, bees, ... all species known. The large number of insect species ... or more factors: a high rate of formation of ... new environments and exploit new ecological niches, and a ...
... No matter how healthy a life one leads, no person ... though the advances of the modern age may have extended ... genetic limits to longevity. One prominent theory of aging lays ... and proteins by “free radicals,?highly reactive molecules produced by the ...
Cached Biology News:Jefferson biologist coaxing human embryonic stem cells to make dopamine with simpler, faster method 2Jefferson biologist coaxing human embryonic stem cells to make dopamine with simpler, faster method 3Islands in the Genome Promote Speciation 2Navigating the brain for sense of direction as paradigm for higher cognitive functions 2
(Date:8/29/2014)... , Aug. 29, 2014 /PRNewswire/ - iCo Therapeutics ... today reported financial results for the six months ... are expressed in Canadian dollars and presented under ... Quarter 2014 Highlights , Announced top-line ... (VA) primary endpoint for subjects enrolled in the ...
(Date:8/29/2014)... 2014 Three Lawrence Livermore researchers ... Hydrogen Production R&D Award for their research ... using sunlight. , Shared with collaborators from the ... of Nevada, Las Vegas (UNLV), the award recognizes ... photoelectrochemical solar-hydrogen production and corrosion processes. , These ...
(Date:8/29/2014)... 2014 A major challenge before new ... dense realm of Boston-Cambridge, is gaining visibility that can ... Sherley, the Director of Boston’s Adult Stem Cell Technology ... national visibility of his company an important priority since ... addition to a social media marketing campaign ...
(Date:8/28/2014)... SAN DIEGO, California, USA (PRWEB) August 28, 2014 ... at the Department of Physics and Optical Science and ... at the University of North Carolina at Charlotte, and ... has been elected to serve as the 2015 Vice ... photonics. , SPIE 2014 President Philip Stahl announced recent ...
Breaking Biology Technology:iCo Therapeutics Announces Second Quarter 2014 Financial Results 2iCo Therapeutics Announces Second Quarter 2014 Financial Results 3iCo Therapeutics Announces Second Quarter 2014 Financial Results 4Livermore Team Awarded for Hydrogen Production Research 2The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 2The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 3The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 4Glenn Boreman, UNC Professor and Plasmonics Co-Founder, Elected to SPIE Presidential Chain 2