Navigation Links
Apo-BrdU Apoptosis Detection Kit from eBioscience

ProductsApo-BrdU Apoptosis Detection Kit from eBioscience
Company eBioscience
Item Apo-BrdU Apoptosis Detection Kit
Price $395.00
Description The APO-BRDU Kit is a 2-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells, including positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl transferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), and fluorescein labeled anti-BrdU antibody for labeling DNA breaks and propidium iodide/RNase A solution for counterstaining the total DNA.

One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. These DNA fragments can be extracted from apoptotic cells and result in the appearance of DNA laddering when the DNA is analyzed by agarose gel electrophoresis. The DNA of non-apoptotic cells that remains largely intact does not display this laddering on agarose gels during electrophoresis. The large number of DNA fragments appearing in apoptotic cells results in a multitude of 3'-hydroxyl termini in the DNA. This property can be used to identify apoptotic cells by labeling the 3'-hydroxyl ends with bromolated deoxyuridine triphosphate nucleotides (Br-dUTP). The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes a template independent addition of deoxyribonucleoside triphosphates to the 3'-hydroxyl ends of double- or single-stranded DNA with either blunt, recessed or overhanging ends. A substantial number of these sites are available in apoptotic cells providing the basis for the method utilized in the APO-BRDU Kit. Recent evidence has demonstrated that Br-dUTP is more readily incorporated into the genome of apoptotic cells than are the deoxynucleotide triphosphates complexed to larger ligands like fluorescein, biotin or digoxigenin. This greater incorporation gives rise to a brighter flow cytometry signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody. Non-apoptotic cells do not incorporate significant amounts of the Br-dUTP due to the lack of exposed 3'-hydroxyl DNA ends.
Info eBioscienceeBioscience
6042 Cornerstone Court West
San Diego, CA 92121
Customer Service: (888) 999-1371
Fax Number: (858) 642-2046
Web Site: http://www.ebioscience.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Apo-Direct Apoptosis Detection Kit from eBioscience
2. ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit from CHEMICON
3. ApopTag Fluorescein In Situ Apoptosis Detection Kit from CHEMICON
4. ApopTag Red In Situ Apoptosis Detection Kit from CHEMICON
5. MitoLight Mitochondrial Apoptosis Detection Kit from CHEMICON
6. MitoLightApoptosis Detection Kit from CHEMICON
7. Apoptosis Pathway SpliceArrays from ExonHit Therapeutics Inc.
8. Flow Cytometry Kit for Apoptosis from Sigma-Aldrich
9. Human Apoptosis Kit 50 tests from BD Biosciences Pharmingen
10. Vybrant Apoptosis Assay Kit #4 from Molecular Probes (Invitrogen)
11. MitoCapture Apoptosis Detection Kit from BioVision
... Edwards (1996) • Provides key ... immunoassays, including RIA, ELISA and ... the photoluminescent and chemiluminescent labels ... also given on assay design ...
EoProbe Eosinophil Staining Kit, 1 Kit...
... and M.W. Turner (1997). ... step-by-step methods for the ... molecules, the use of ... analytical probes and as ...
B.D. Hames and D.M. Glover (1996) • This issue covers T-Cell antigen receptor genes; T-Lymphocyte signal transduction; B-Cell activation antibody engineering and the complement system and other ...
Biology Products:
(Date:5/16/2017)...   Bridge Patient Portal , an enterprise ... EMR Systems , an electronic medical record solutions ... established a partnership to build an interface between ... Centricity™ products, including Centricity Practice Solution (CPS), Centricity ... new integrations will allow healthcare delivery networks using ...
(Date:4/17/2017)... NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or the "Company"), ... Report on Form 10-K on Thursday April 13, 2017 with the ... The ... section of the Company,s website at http://www.nxt-id.com  under "SEC Filings," ... 2016 Year Highlights: Acquisition of ...
(Date:4/11/2017)... N.Y. , April 11, 2017 ... fingerprints, but researchers at the New York University ... College of Engineering have found that partial similarities ... security systems used in mobile phones and other ... thought. The vulnerability lies in the ...
Breaking Biology News(10 mins):
(Date:7/17/2017)... (PRWEB) , ... July 17, ... ... of technology-enabled clinical trial solutions, today announced safety software company AB Cube ... the eHealth Solutions business segment to advance technology innovation across life sciences ...
(Date:7/17/2017)... , ... July 17, 2017 , ... ... characterized by a wide range of overlapping clinical features. The advancement of targeted ... the field of NDD research and testing. , However, designing a custom ...
(Date:7/17/2017)... ... July 17, 2017 , ... Co-Diagnostics, Inc. ... and intends both to manufacture and sell reagents used for diagnostic tests, has ... , Headquartered in Sandy, Utah, Co-Diagnostics’ intellectual property and technologies are protected by ...
(Date:7/16/2017)... (PRWEB) , ... July 16, 2017 , ... ... equipment and analytical instruments announced the launch of its new line of Rocking ... five rocking and waving shaker models (both analog and digital) for laboratory applications ...
Breaking Biology Technology: