|Products||Anti-Rat HRP-DAB Cell & Tissue Staining Kit from R&D Systems|
|Item||Anti-Rat HRP-DAB Cell & Tissue Staining Kit|
|Description|| R&D Systems Cell and Tissue Staining Kits are intended for localization of antigens in a broad range of histological and cytological specimens. These kits may be used with primary IgG antibodies manufactured by either R&D Systems or by other vendors. Briefly, detection is based on the formation of the Avidin-Biotin Complex (ABC) with primary antibodies that reacted with tissue antigens under study. Visualization is based on enzymatic conversion of a chromogenic substrate 3,3 Diaminobenzidine (DAB) into a colored brown precipitate by horseradish peroxidase (HRP) at the sites of antigen localization, which can then be viewed using bright-field microscopy. The high detection sensitivity of R&D Systems staining kits is achieved by using high quality Secondary Biotinylated Antibodies and High Sensitivity Streptavidin conjugated to HRP (HSS-HRP). High Sensitivity Streptavidin is a chemical analog of Streptavidin that has little net positive charge at neutral or slightly alkaline pH and will interact only with biotin attached to secondary antibodies. HSS-HRP shows little or no non-specific binding to phospholipids, nucleic acids and carbohydrate binding proteins.|
R&D Systems Cell and Tissue Staining Kits are manufactured to work equally well on cryostat and paraffin-embedded tissue sections, cytospin preparations (i.e. lymphocytes, monocytes, transfected cells) and free-floating tissue sections. The kits are made in a ready-to-use format including pre-diluted Secondary Biotinylated Antibodies and HSS-HRP that eliminates the need for extra steps in the procedure, minimizes hands-on time, maximizes convenience and reduces the risk of erroneous calculations.
|Info|| R&D Systems|
R&D Systems, Inc.
614 McKinley Place N.E.
Minneapolis, MN 55413
Customer Service: 1 (800) 343-7475
1 (612) 379-2956
Fax Number: 1 (612) 656-4400
Web Site: http://www.rndsystems.com