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Anti-Rat HRP-AEC Cell & Tissue Staining Kit from R&D Systems

ProductsAnti-Rat HRP-AEC Cell & Tissue Staining Kit from R&D Systems
Company R&D Systems
Item Anti-Rat HRP-AEC Cell & Tissue Staining Kit
Price $190.00
Description R&D Systems Cell and Tissue Staining Kits are intended for localization of antigens in a broad range of histological and cytological specimens. These kits may be used with primary IgG antibodies manufactured by either R&D Systems or by other vendors. Briefly, detection is based on the formation of the Avidin-Biotin Complex (ABC) with primary antibodies that reacted with tissue antigens under study. Visualization is based on enzymatic conversion of a chromogenic substrate 3,3 Diaminobenzidine (DAB) into a colored brown precipitate by horseradish peroxidase (HRP) at the sites of antigen localization, which can then be viewed using bright-field microscopy. The high detection sensitivity of R&D Systems staining kits is achieved by using high quality Secondary Biotinylated Antibodies and High Sensitivity Streptavidin conjugated to HRP (HSS-HRP). High Sensitivity Streptavidin is a chemical analog of Streptavidin that has little net positive charge at neutral or slightly alkaline pH and will interact only with biotin attached to secondary antibodies. HSS-HRP shows little or no non-specific binding to phospholipids, nucleic acids and carbohydrate binding proteins.

R&D Systems Cell and Tissue Staining Kits are manufactured to work equally well on cryostat and paraffin-embedded tissue sections, cytospin preparations (i.e. lymphocytes, monocytes, transfected cells) and free-floating tissue sections. The kits are made in a ready-to-use format including pre-diluted Secondary Biotinylated Antibodies and HSS-HRP that eliminates the need for extra steps in the procedure, minimizes hands-on time, maximizes convenience and reduces the risk of erroneous calculations.

  • Species: &nbnsp; Rat
Info R&D SystemsR&D Systems
R&D Systems, Inc.
614 McKinley Place N.E.
Minneapolis, MN 55413
Customer Service: 1 (800) 343-7475
1 (612) 379-2956

Fax Number: 1 (612) 656-4400
Web Site: http://www.rndsystems.com
NOTE: Price information is approximate list price and actual prices may vary.

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... Target Retrieval Solution, High pH ... heat-induced target retrievel prior to immunohistochemical staining ... paraffin-embedded tissue sections mounted on glass slides. ... pH, 9.9, is especially effective. It should ...
... potent Rnase free Ribonuclease Inhibitor ... broad range of temperatures and ... not inhibit SP6, T7, or ... M-MLV reverse transcriptase or Taq ...
... Target Retrieval Solution, pH 9 (x 10) ... pH 9, intended for heat-induced target retrieval ... well-suited for use on formalin-fixed, paraffin-embedded tissue ... 0.01 mol/L citrate buffer, pH 6, the ...
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