Navigation Links
Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

ProductsAmylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item Amylase Fluorometric (Green) Assay Kit, EnzChek®
Price 
Description α-Amylase is a hydrolytic enzyme that catalyzes the conversion of starch to a mixture of glucose, maltose, maltotriose and dextrins. The level of a-amylase in various fluids of the human body are of clinical importance,1-3 while plant and microbial a-amylases are important enzymes for industry.4

The EnzChek Amylase Assay Kit (E-11954) provides the speed, high sensitivity and convenience required for measuring a-amylase activity or for screening inhibitors in a high-throughput format. The EnzChek kit contains a starch derivative that is labeled with the BODIPY FL dye to such a degree that the fluorescence is quenched. The DQ starch substrate is efficiently degraded by a-amylase to yield highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader, minifluorometer or standard fluorometer.

α-Amylase purified from Bacillus sp. is provided with the EnzChek Amylase Assay Kit to serve as a control enzyme. Using 12.5 g/mL of the DQ starch substrate and a 60 minute incubation period at room temperature, the assay can detect the activity of this enzyme down to a final concentration of 1 10-4 U/mL (0.3 g protein/mL), where one unit is defined as the amount of enzyme required to liberate 1 mg of maltose from starch in 3 minutes at 20C, pH 6.9. At high enzyme concentrations (e.g. 2 mU/mL and higher), incubation times can be as short as 5 minutes.

Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
PO Box 22010
Eugene, OR 97402-0469

Call Molecular Probes (Invitrogen) to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-541-359-2658
Customer Service: 1-541-465-8300
Fax Number: 1-541-344-6504
Web Site: http://probes.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
2. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
3. 20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON
4. Cell invasion Fluorometric (green) Assay Kit, InnoCyte™ from Calbiochem
5. Telomerase Fluorometric Assay Kit, TRAPeze from CHEMICON
6. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
7. TACE Fluorometric (MCA)) Assay Kit, InnoZyme™ from Calbiochem
8. Lysozyme Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
9. Cholesterol Fluorometric (Red) Assay Kit, Amplex Red from Molecular Probes (Invitrogen)
10. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
11. MMP-1 Fluorometric (Blue) Assay Kit, EnzoLyte from AnaSpec
... handy dispensers save space and organize ... other garb. They are the perfect ... used in labs, processing and manufacturing. ... and the particles they attract. Many ...
... that it uses a deep-UV deuterium bulb, which ... with a shutter (controlled via a TTL signal ... Source produces a powerful, stable output from 215-400 ... source are available, providing a wavelength range of ...
... In situ detection of apoptosis and mitochondrial ... provides an early indication of the initiation ... defined as a collapse in the electrochemical ... by the change in the membrane potential ...
... an exciting product for cell-based kinetic assays ... the use of expensive HTS equipment, which ... the assay development stage. The NOVOstar offers ... as well as any other assay suitable ...
Biology Products:
(Date:9/2/2014)... (September 2, 2014) In the battle between our ... unknown ally in bacteria-killing viruses known as phages. In ... Hospital, Partners In Health, Haiti,s National Public Health Laboratory, ... to give up their virulence in order to survive. ... found that cholera,s mutational escape from phage predation ...
(Date:9/2/2014)... September 2, 2014 A study published in ... of the American Academy of Child and Adolescent ... with a psychiatric diagnosis had an increased risk ... antipsychotics. , Using data from the nationwide Danish ... Ren Ernst Nielsen, Psychiatry, Aalborg University Hospital, Denmark, ...
(Date:9/2/2014)... their long, spiky legs and their propensity for eating ... of nightmares. And now research from North Carolina State ... into homes across the eastern United States. , "The ... pose any kind of threat to humans," says Dr. ... and lead author of a paper about the research. ...
Breaking Biology News(10 mins):War between bacteria and phages benefits humans 2War between bacteria and phages benefits humans 3Risk of diabetes in children and adolescents exposed to antipsychotics 2Researchers find Asian camel crickets now common in US homes 2
... you ever met a cat that doesn't go crazy over ... the world don't respond to it at all, according to ... of Chemical & Engineering News, the weekly newsmagazine published by ... Catnip sensitivity is inherited, says Carolyn M. McDaniel, a veterinarian ...
... Florida researchers say they are a step closer to ... cancers before symptoms become apparent. , The findings, currently ... Sciences, involve introducing molecularly engineered strands of DNA into ... burst after they adhere to cancer proteins. , ...
... the gene responsible for controlling a first key step in ... in the journal Nature tomorrow (Thursday 27 October 2005). , ... take place once a sperm enters an egg, giving it ... reproducing animals. , The absence or mutation of this gene ...
Cached Biology News:Many cats unaffected by catnip, C&EN reports 2Scientists closer to new cancer detection method 2Scientists closer to new cancer detection method 3Scientists unpick genetics of first 15 minutes of life 2
(Date:9/2/2014)... , Sept. 2, 2014  Technology Applications International Corporation ... its wholly-owned subsidiary Renuell Int,l, Inc. has signed an ... Space Administration ("NASA") at the Johnson Space Center in ... See photos: tapplic.com/houstonphotos/ ... very pleased to announce our latest, exclusive agreement with ...
(Date:9/2/2014)... September 02, 2014 Opertech Bio, ... highly efficient, cost effective approach to sensory evaluation, ... 8,820,265 by the U.S. Patent and Trademark Office, ... , “Opertech’s taste evaluation technology called ... automated high throughput system for rapid characterization of ...
(Date:9/2/2014)... 2014  Xencor, Inc. (NASDAQ: XNCR ... antibodies for the treatment of autoimmune diseases, asthma ... appointment of Debra Zack , M.D., Ph.D., ... Lloyd Rowland , senior vice president, chief ... "Debra,s expertise in biologics clinical development and medical ...
(Date:9/2/2014)... , Sept. 2, 2014  Synthetic Biologics, Inc. ... biologic and drug candidates targeting specific pathogens that ... results from its final preclinical toxicology study of ... guidance, this bridging study was required to move ... to prevent the devastating effects of Clostridium ...
Breaking Biology Technology:Technology Applications International Corporation Subsidiary Enters Into Additional Exclusive Agreement with NASA at a Signing Ceremony at the Johnson Space Center In Houston, Texas 2Technology Applications International Corporation Subsidiary Enters Into Additional Exclusive Agreement with NASA at a Signing Ceremony at the Johnson Space Center In Houston, Texas 3Opertech Bio Receives U.S. Patent for High-Throughput Taste Evaluation Technology 2Xencor Appoints Debra Zack, M.D., Ph.D., Vice President, Clinical Development and Lloyd Rowland, Senior Vice President, Chief Compliance Officer and General Counsel 2Xencor Appoints Debra Zack, M.D., Ph.D., Vice President, Clinical Development and Lloyd Rowland, Senior Vice President, Chief Compliance Officer and General Counsel 3Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 2Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 3Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 4Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 5