Navigation Links
Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

ProductsAmylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item Amylase Fluorometric (Green) Assay Kit, EnzChek®
Price 
Description α-Amylase is a hydrolytic enzyme that catalyzes the conversion of starch to a mixture of glucose, maltose, maltotriose and dextrins. The level of a-amylase in various fluids of the human body are of clinical importance,1-3 while plant and microbial a-amylases are important enzymes for industry.4

The EnzChek Amylase Assay Kit (E-11954) provides the speed, high sensitivity and convenience required for measuring a-amylase activity or for screening inhibitors in a high-throughput format. The EnzChek kit contains a starch derivative that is labeled with the BODIPY FL dye to such a degree that the fluorescence is quenched. The DQ starch substrate is efficiently degraded by a-amylase to yield highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader, minifluorometer or standard fluorometer.

α-Amylase purified from Bacillus sp. is provided with the EnzChek Amylase Assay Kit to serve as a control enzyme. Using 12.5 g/mL of the DQ starch substrate and a 60 minute incubation period at room temperature, the assay can detect the activity of this enzyme down to a final concentration of 1 10-4 U/mL (0.3 g protein/mL), where one unit is defined as the amount of enzyme required to liberate 1 mg of maltose from starch in 3 minutes at 20C, pH 6.9. At high enzyme concentrations (e.g. 2 mU/mL and higher), incubation times can be as short as 5 minutes.

Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
PO Box 22010
Eugene, OR 97402-0469

Call Molecular Probes (Invitrogen) to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-541-359-2658
Customer Service: 1-541-465-8300
Fax Number: 1-541-344-6504
Web Site: http://probes.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
2. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
3. 20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON
4. Cell invasion Fluorometric (green) Assay Kit, InnoCyte™ from Calbiochem
5. Telomerase Fluorometric Assay Kit, TRAPeze from CHEMICON
6. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
7. TACE Fluorometric (MCA)) Assay Kit, InnoZyme™ from Calbiochem
8. Lysozyme Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
9. Cholesterol Fluorometric (Red) Assay Kit, Amplex Red from Molecular Probes (Invitrogen)
10. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
11. MMP-1 Fluorometric (Blue) Assay Kit, EnzoLyte from AnaSpec
High affinity antibodies recognizes HSP70 protein from human cardiac tissue. Could be used for Westerb blotting application, ELISA....
... kit I provides reagents and a protein standard ... reducing agents and detergents. The RC DC protein ... reagents and standards sufficient for 500 standard assays: ... reagent II, 250 ml alkaline copper tartrate solution, ...
5X StabilCoat Immunoassay Stabilizer is a 5X concentrate solution of StabilCoat Immunoassay Stabilizer. The shelf-life of 5X StabilCoat Immunoassay Stabilizer is 2 years when stored at 4C. Product ...
... for determining the absolute count of CD34+ ... peripheral blood samples, and leukapheresis samples. These ... cell count and a total CD45 (leucocyte) ... use in the BD Procount progenitor cell ...
Biology Products:
(Date:2/28/2017)... ANGELES , Feb. 28, 2017   Acuant ... verification software globally, announces significant enhancements to new and ... May 2016. New products include mobile and desktop Acuant ... DocX TM - a real time manual review ... core idScan® technology provides the fastest and most accurate ...
(Date:2/27/2017)... WASHINGTON , Feb. 27, 2017   Strategic ... fund, today announced it has led a $3.5 million investment ... collaboration platform. Strategic Cyber Ventures is DC based and ... and Hank Thomas . Ron Gula , ... Tech Ventures, also participated in this series A round ...
(Date:2/22/2017)... LONDON , Feb. 22, 2017 ... by 2021, ABI Research identifies four technologies that ... solutions to secure significant share in the changing ... payments, and passive authentication.   "Companies ... it comes to security," says Dimitrios Pavlakis ...
Breaking Biology News(10 mins):
(Date:3/28/2017)... ... March 28, 2017 , ... ... for the Advancement of Science (AAAS), the world's largest general scientific society ... its high-impact scholarly collection across its cross-platform reference management system. , All ...
(Date:3/28/2017)... (PRWEB) , ... March 28, 2017 , ... Mass spectrometry ... The utilization of this technology is driven by its potential to perform challenging analyses ... are also some challenges that must be addressed for it to be routinely used ...
(Date:3/28/2017)... Philadelphia, PA (PRWEB) , ... March 28, 2017 ... ... Johnston, spoke at a Marketing Roundtable on March 22 in Philadelphia. The event ... WHYY. , The Marketing Roundtable featured breakout groups and interaction ...
(Date:3/28/2017)... ... March 28, 2017 , ... Triumph Modular Incorporated, and ... (MBI), an international modular trade organization, were awarded First Place, as well as ... Life Lab at Harvard University. The awards were presented at the 34th Annual ...
Breaking Biology Technology: