Navigation Links
Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

ProductsAmylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item Amylase Fluorometric (Green) Assay Kit, EnzChek®
Price 
Description a-Amylase is an enzyme that catalyzes the hydrolysis of starch to a mixture of maltose, maltotriose, and dextrins. The level of a-amylase in various fluids of the human body is of clinical significance in the diagnosis of disease states including pancreatitis and diabetes; plant and microbial a-amylases are important enzymes for industrial applications ranging from the manufacture of baked goods and dairy products to the production of ethanol and paper.14

The EnzChek Ultra Amylase Assay Kit (E33651) provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek kit contains a starch derivative the DQ starch substrate that is labeled with BODIPY FL dye (Figure 1) to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity (Figures 2 and 3) and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.

In tests using a-amylase from Bacillus sp. (Sigma A-6380) and 200 g/mL of the DQ starch substrate (30 minute incubation at room temperature), the EnzChek Ultra Amylase Assay Kit could be used to detect a-amylase activity down to a final concentration of 2 10-3 U/mL, where one unit is defined as the amount of enzyme required to liberate 1 mg of maltose from starch in 3 minutes at 20C, pH 6.9.

Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
PO Box 22010
Eugene, OR 97402-0469

Call Molecular Probes (Invitrogen) to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-541-359-2658
Customer Service: 1-541-465-8300
Fax Number: 1-541-344-6504
Web Site: http://probes.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
2. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
3. 20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON
4. Cell invasion Fluorometric (green) Assay Kit, InnoCyte™ from Calbiochem
5. Telomerase Fluorometric Assay Kit, TRAPeze from CHEMICON
6. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
7. TACE Fluorometric (MCA)) Assay Kit, InnoZyme™ from Calbiochem
8. Lysozyme Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
9. Cholesterol Fluorometric (Red) Assay Kit, Amplex Red from Molecular Probes (Invitrogen)
10. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
11. MMP-1 Fluorometric (Blue) Assay Kit, EnzoLyte from AnaSpec
... carry-over contamination, which prevents false positive ... from previous PCR amplifications without degrading ... amplification ,Optimized for use with GeneAmp ... systems , The GeneAmp PCR Carry-over ...
... enzyme that catalyzes the conversion,of starch ... maltotriose and,dextrins. The level of a-amylase ... are of clinical importance,1-3 while plant ... industry.4, The EnzChek Amylase Assay Kit ...
... that catalyzes the hydrolysis of,starch to ... dextrins. The,level of a-amylase in various ... of,clinical significance in the diagnosis of ... and microbial a-amylases are,important enzymes for ...
Rat monoclonal [20d5] to NKG2A + C+ E ( Abpromise for all tested applications). Antigen: Tissue / cell preparation (Mouse): CHO transfected cells expressing the B6 allele of NKG2A. Entrez Gen...
Biology Products:
(Date:11/21/2014)... C-Labs LLC, a leading provider of remote ... today announced the appointment of John Traynor ... strategic advisor to the firm, Mr. Traynor will now ... based out of the C-Labs office in ... Muench , Chief Executive Officer. Photo - ...
(Date:11/18/2014)... , Nov. 18, 2014  The Secure ... Industry Association today jointly announce the formation of ... release of its Identity and Biometric Entry ... The Framework received official support from BORDERPOL, the ... to improve and provide expertise regarding border security, ...
(Date:11/18/2014)... Md. , Nov. 17, 2014 The Parenteral ... and U.S. regulatory agencies will speak and at least seven ... Metrics Conference at the Omni Shoreham Hotel in ... pleased once again to have significant support from the regulatory ... Europe in our effort to help ...
Breaking Biology News(10 mins):C-Labs Names Former Microsoft and Bsquare Executive as Chief Operating Officer 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 3FDA's Janet Woodcock, EMA's Emer Cooke Headline PDA Quality Metrics Conference 2
... Atlantic right whale's future looks grim if the current ... professor of oceanography Douglas Nowacek and a group of ... co-authored a paper titled "North Atlantic Right Whales in ... the journal Science. , In it, Nowacek and his ...
... the final step in the completion of new blood vessel ... Diego (UCSD) School of Medicine team in research that promises ... by cutting off the tumor blood supply. , The research ... at UCSD and a member of the Rebecca and John ...
... Scientists may have found a way to dramatically slow ... , That's the promising implication of an animal study ... at Birmingham (UAB) and the University of Florida (UF) ... the National Academy of Sciences. , The research team ...
Cached Biology News:FSU scientist warns North Atlantic right whale facing extinction 2Unexpected lock and key mechanism found for the assembly of tumor blood vessels 2Unexpected lock and key mechanism found for the assembly of tumor blood vessels 3Discoveries by UAB and Florida scientists may help transplanted organs survive longer 2
(Date:11/22/2014)... 2014   , ... Officer zu KLOX    ... Financial Officer bestellt    KLOX ist ... jüngst zugelassenen Behandlungssystems zur Wundheilung zu beginnen    , ... ist sehr erfreut, die folgenden personellen Veränderungen anzukündigen: ...
(Date:11/21/2014)... 20, 2014 Author Matthew J. Pallamary’s ... the Other Side” published as a tribute to ... the “Fiction: Short Story” category of the 2014 ... and CEO of USA Book News, said this year’s ... publishers, including Simon & Schuster, Penguin, John Wiley & ...
(Date:11/21/2014)... November 21, 2014 Why did Stephen ... Albert Einstein so instantly recognizable? Why have they become ... astronomer and author Hilton Ratcliffe seeks out the answers ... at all to do with science. In " Stephen ... , November 21, 2014), Ratcliffe puts it plainly: ...
(Date:11/21/2014)... YORK and SAN DIEGO , ... ), today announced that President and CEO Marc Hedrick , ... DATE:   Thursday, December 4, 2014 TIME: ... Click here or paste this URL into your ... recommended that investors pre-register to save time and receive event ...
Breaking Biology Technology:KLOX Technologies meldet Neubesetzung von Führungspositionen 2KLOX Technologies meldet Neubesetzung von Führungspositionen 3KLOX Technologies meldet Neubesetzung von Führungspositionen 4KLOX Technologies meldet Neubesetzung von Führungspositionen 5Mystic Ink 's Short Story Collection “A Short Walk to the Other Side” Honoring Ray Bradbury Praised As Award-Winning Finalist In USA Best Book Awards 2Mystic Ink 's Short Story Collection “A Short Walk to the Other Side” Honoring Ray Bradbury Praised As Award-Winning Finalist In USA Best Book Awards 3Mystic Ink 's Short Story Collection “A Short Walk to the Other Side” Honoring Ray Bradbury Praised As Award-Winning Finalist In USA Best Book Awards 4“Stephen Hawking Smoked My Socks” Sparks Controversy in Science World 2“Stephen Hawking Smoked My Socks” Sparks Controversy in Science World 3“Stephen Hawking Smoked My Socks” Sparks Controversy in Science World 4Cytori Therapeutics to Provide Corporate Update and Investor Presentation on December 4 through a Live, Interactive Webcast 2Cytori Therapeutics to Provide Corporate Update and Investor Presentation on December 4 through a Live, Interactive Webcast 3Cytori Therapeutics to Provide Corporate Update and Investor Presentation on December 4 through a Live, Interactive Webcast 4