Navigation Links
Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

ProductsAmylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item Amylase Fluorometric (Green) Assay Kit, EnzChek®
Price 
Description a-Amylase is an enzyme that catalyzes the hydrolysis of starch to a mixture of maltose, maltotriose, and dextrins. The level of a-amylase in various fluids of the human body is of clinical significance in the diagnosis of disease states including pancreatitis and diabetes; plant and microbial a-amylases are important enzymes for industrial applications ranging from the manufacture of baked goods and dairy products to the production of ethanol and paper.14

The EnzChek Ultra Amylase Assay Kit (E33651) provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek kit contains a starch derivative the DQ starch substrate that is labeled with BODIPY FL dye (Figure 1) to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity (Figures 2 and 3) and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.

In tests using a-amylase from Bacillus sp. (Sigma A-6380) and 200 g/mL of the DQ starch substrate (30 minute incubation at room temperature), the EnzChek Ultra Amylase Assay Kit could be used to detect a-amylase activity down to a final concentration of 2 10-3 U/mL, where one unit is defined as the amount of enzyme required to liberate 1 mg of maltose from starch in 3 minutes at 20C, pH 6.9.

Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
PO Box 22010
Eugene, OR 97402-0469

Call Molecular Probes (Invitrogen) to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-541-359-2658
Customer Service: 1-541-465-8300
Fax Number: 1-541-344-6504
Web Site: http://probes.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
2. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
3. 20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON
4. Cell invasion Fluorometric (green) Assay Kit, InnoCyte™ from Calbiochem
5. Telomerase Fluorometric Assay Kit, TRAPeze from CHEMICON
6. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
7. TACE Fluorometric (MCA)) Assay Kit, InnoZyme™ from Calbiochem
8. Lysozyme Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
9. Cholesterol Fluorometric (Red) Assay Kit, Amplex Red from Molecular Probes (Invitrogen)
10. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
11. MMP-1 Fluorometric (Blue) Assay Kit, EnzoLyte from AnaSpec
... Western Blot Recycling Kit is effective ... blots that have been developed with ... isotopes. It is not recommended for ... etc.), as it is not possible ...
... Immulon 2HB ELISA strips, ... increased binding affinity for hydrophilic proteins ... works well for reagent injection while ... Each strip accomodates 12 ...
Ethylene glycol-bis(2-aminoethyl ether)-N,N,N',N'-tetraacetic acid, Molecular Biology Grade...
... and negatively supercoiled DNA in presence of EDTA ... effects of supercoiling on transcription in vitro ... Determining the degree of supercoiling of naturally occurring ... in length by only one base-pair , ...
Biology Products:
(Date:6/2/2016)... June 2, 2016 Perimeter Surveillance ... Unmanned Systems, Physical Infrastructure, Support & Other Service  ... visiongain offers comprehensive analysis of the global ... will generate revenues of $17.98 billion in 2016. ... Inc, a leader in software and hardware technologies for ...
(Date:5/24/2016)... superior patient care by providing unparalleled technology to leaders of the medical imaging industry. ... recently added to the range of products distributed by Ampronix. Photo - ... ... ... ...
(Date:5/16/2016)... May 16, 2016   EyeLock LLC , a ... the opening of an IoT Center of Excellence in ... expand the development of embedded iris biometric applications. ... of convenience and security with unmatched biometric accuracy, making ... aside from DNA. EyeLock,s platform uses video technology to ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... , June 24, 2016  Regular discussions on a range ... place between the two entities said Poloz. Speaking ... Ottawa , he pointed to the country,s inflation ... federal government. "In ... "Both institutions have common economic goals, why not sit down ...
(Date:6/23/2016)... ... June 23, 2016 , ... UAS LifeSciences, one of the ... brand, UP4™ Probiotics, into Target stores nationwide. The company, which has been manufacturing ... to its list of well-respected retailers. This list includes such fine stores as ...
(Date:6/23/2016)... ... June 23, 2016 , ... Charm Sciences, Inc. is pleased ... received AOAC Research Institute approval 061601. , “This is another AOAC-RI approval of ... Salter, Vice President of Regulatory and Industrial Affairs. “The Peel Plate methods perform ...
(Date:6/23/2016)... ... ... STACS DNA Inc., the sample tracking software company, today announced that Dr. Hays ... DNA as a Field Application Specialist. , “I am thrilled that Dr. Young ... DNA. “In further expanding our capacity as a scientific integrator, Hays brings a wealth ...
Breaking Biology Technology: