| Products | Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen) | |
| Company | Molecular Probes (Invitrogen) | |
| Item | Amylase Fluorometric (Green) Assay Kit, EnzChek® | |
| Price | ||
| Description | a-Amylase is an enzyme that catalyzes the hydrolysis of
starch to a mixture of maltose, maltotriose, and dextrins. The
level of a-amylase in various fluids of the human body is of
clinical significance in the diagnosis of disease states including
pancreatitis and diabetes; plant and microbial a-amylases are
important enzymes for industrial applications ranging from the
manufacture of baked goods and dairy products to the production
of ethanol and paper.14
The EnzChek Ultra Amylase Assay Kit (E33651) provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek kit contains a starch derivative the DQ starch substrate that is labeled with BODIPY FL dye (Figure 1) to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity (Figures 2 and 3) and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters. In tests using a-amylase from Bacillus sp. (Sigma A-6380) and 200 g/mL of the DQ starch substrate (30 minute incubation at room temperature), the EnzChek Ultra Amylase Assay Kit could be used to detect a-amylase activity down to a final concentration of 2 10-3 U/mL, where one unit is defined as the amount of enzyme required to liberate 1 mg of maltose from starch in 3 minutes at 20C, pH 6.9. | |
| Info | Molecular Probes (Invitrogen)PO Box 22010 Eugene, OR 97402-0469
Fax Number: 1-541-344-6504 Web Site: http://probes.invitrogen.com |