Navigation Links
Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

ProductsAmylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item Amylase Fluorometric (Green) Assay Kit, EnzChek®
Price 
Description a-Amylase is an enzyme that catalyzes the hydrolysis of starch to a mixture of maltose, maltotriose, and dextrins. The level of a-amylase in various fluids of the human body is of clinical significance in the diagnosis of disease states including pancreatitis and diabetes; plant and microbial a-amylases are important enzymes for industrial applications ranging from the manufacture of baked goods and dairy products to the production of ethanol and paper.14

The EnzChek Ultra Amylase Assay Kit (E33651) provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek kit contains a starch derivative the DQ starch substrate that is labeled with BODIPY FL dye (Figure 1) to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity (Figures 2 and 3) and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.

In tests using a-amylase from Bacillus sp. (Sigma A-6380) and 200 g/mL of the DQ starch substrate (30 minute incubation at room temperature), the EnzChek Ultra Amylase Assay Kit could be used to detect a-amylase activity down to a final concentration of 2 10-3 U/mL, where one unit is defined as the amount of enzyme required to liberate 1 mg of maltose from starch in 3 minutes at 20C, pH 6.9.

Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
PO Box 22010
Eugene, OR 97402-0469

Call Molecular Probes (Invitrogen) to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-541-359-2658
Customer Service: 1-541-465-8300
Fax Number: 1-541-344-6504
Web Site: http://probes.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
2. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
3. 20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON
4. Cell invasion Fluorometric (green) Assay Kit, InnoCyte™ from Calbiochem
5. Telomerase Fluorometric Assay Kit, TRAPeze from CHEMICON
6. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
7. TACE Fluorometric (MCA)) Assay Kit, InnoZyme™ from Calbiochem
8. Lysozyme Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
9. Cholesterol Fluorometric (Red) Assay Kit, Amplex Red from Molecular Probes (Invitrogen)
10. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
11. MMP-1 Fluorometric (Blue) Assay Kit, EnzoLyte from AnaSpec
PTC-100 cycler with gold/silver block with Hot Bonnet heated lid, holds one 96-well V-plate or96x0.2ml tubes...
... performance on minimum space. Thanks to a ... and cooling rates have been achieved that, ... maximum capacity of 48 x 0.2ml tubes, ... much more expensive 96 well instruments. Alternatively, ...
... Super Sensitive Polymer-HRP IHC ... novel product based on ... technology. This system yields ... noise ratio and minimizes ...
... Cycler unit is an inexpensive personal or mini ... PCR process. Lightweight, with a small footprint, it ... including the researcher's desk. , The Gene Cycler ... do not have access to a large laboratory ...
Biology Products:
(Date:3/9/2017)... Australia , March 9, 2017 /PRNewswire/ ... at the prestigious World Lung Imaging Workshop at the ... Fouras , was invited to deliver the latest data ... This globally recognised event brings together leaders at the ... latest developments in lung imaging. "The ...
(Date:3/6/2017)... , March 6, 2017 Mintigo ... technology, today announced Predictive Sales Coach TM , ... actionable sales intelligence into Salesforce. This unique AI ... their sales organizations with deep knowledge of their ... for intelligent engagement. Predictive Sales Coach extends Mintigo,s ...
(Date:3/2/2017)... , March 2, 2017 Australian stem cell ... (ASX: CYP), has signed an agreement with the Monash ... Monash Biomedicine Discovery Institute and Department of Pharmacology at ... a further preclinical study to support the use of ... asthma.  Asthma is a chronic, long ...
Breaking Biology News(10 mins):
(Date:3/28/2017)... ... 28, 2017 , ... Franz Inc ., the leading supplier of Semantic ... named a ‘Champion’ by Bloor Research in its recent Graph Database Market Update ... and, thanks to Gruff, it was rated as the easiest product to use.” – ...
(Date:3/28/2017)... ... March 28, 2017 , ... Ecovia ... Business Innovation Research (SBIR) grant from the National Science Foundation (NSF). Under the ... to develop a suite of BioGel™ biopolymer materials for hygiene applications, particularly for ...
(Date:3/28/2017)... Summary This report provides all the information ... interests and activities since 2010. Description The Partnering Deals ... the partnering activity of one of the world,s leading life ... upon purchase to ensure inclusion of the most up to ... be delivered in PDF format within 1 working day of ...
(Date:3/28/2017)... March 28, 2017 Volition America, Inc., a wholly-owned ... the engagement of Deborah Vollmer Dahlke , DrPH, CEO ... Dr. Vollmer Dahlke,s role will be to assist ... State of Texas and elsewhere in ... Dahlke has significant experience over the past six years, ...
Breaking Biology Technology: