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AmpliTaq Gold DNA Polymerase from Applied Biosystems

ProductsAmpliTaq Gold DNA Polymerase from Applied Biosystems
Company Applied Biosystems
Item AmpliTaq Gold DNA Polymerase
Description AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase. When the chemical moiety is attached to the enzyme, the enzyme is inactive. During set-up and the first ramp of thermal cycling (when the reaction is going through non-optimal annealing temperatures), the enzyme is inactive. The result is that mis-primed primers are not extended. Once the reaction reaches optimal annealing temperatures, the chemical moiety is cleaved during a heat activation step, releasing active AmpliTaq® DNA enzyme.

The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot start enzyme, there is no worry of biological contamination.

AmpliTaq Gold® DNA enzyme's chemical hot-start capability can release active enzyme in a "time-release" manner. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity.
Info Applied BiosystemsApplied Biosystems
850 Lincoln Centre Drive
Foster City, CA 94404

Call Applied Biosystems to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-650-425-3266
Customer Service: 650-638-5800
Fax Number: 650-638-5884
Web Site:
NOTE: Price information is approximate list price and actual prices may vary.

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