|Products||AmpliTaq Gold DNA Polymerase from Applied Biosystems|
|Item||AmpliTaq Gold DNA Polymerase|
|Description|| AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase. When the chemical moiety is attached to the enzyme, the enzyme is inactive. During set-up and the first ramp of thermal cycling (when the reaction is going through non-optimal annealing temperatures), the enzyme is inactive. The result is that mis-primed primers are not extended. Once the reaction reaches optimal annealing temperatures, the chemical moiety is cleaved during a heat activation step, releasing active AmpliTaq® DNA enzyme.|
The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot start enzyme, there is no worry of biological contamination.
AmpliTaq Gold® DNA enzyme's chemical hot-start capability can release active enzyme in a "time-release" manner. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity.
|Info|| Applied Biosystems|
850 Lincoln Centre Drive
Foster City, CA 94404
Fax Number: 650-638-5884
Web Site: http://home.appliedbiosystems.com/