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AcTEV Protease from Invitrogen

ProductsAcTEV Protease from Invitrogen
Company Invitrogen
Item AcTEV Protease
Description AcTEV Protease is an enhanced form of Tobacco Etch Virus (TEV) protease that is highly site-specific, highly active, and significantly more stable than native TEV protease, resulting in long-term activity. AcTEV Protease specifically recognizes a seven amino acid (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly), making it useful for removing affinity tags from fusion proteins (1,2). For proper cleavage, the protein of interest is expressed with an AcTEV (TEV) Protease recognition sequence located between it and the affinity tag. Subsequent incubation with AcTEV Protease releases the protein of interest from the fusion tag. This is an effective way to remove solubility, secretion, detection, and purification tags from recombinant proteins. AcTEV Protease features

Highly specific cleavage activity at the TEV recognition site

  • Enhanced enzyme stability for prolonged protease activity
  • Activity over a broad temperature range (+4C to 30C)
  • Activity over a broad pH range (pH 6.0 to 8.5)
  • A six-histidine sequence to facilitate its removal from the digested protein sample
  • Greater than 85% single-band purity with no non-specific protease contamination
Info InvitrogenInvitrogen
Invitrogen Corporation
1600 Faraday Ave.
Carlsbad, CA 92008

Call Invitrogen to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-521-4270
Customer Service: 800-955-6288
Tech Support: 800-955-6288 ext. 2
Fax Number: 760-603-7229
Web Site:
NOTE: Price information is approximate list price and actual prices may vary.

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Mol wt: average mol wt16,951.27 Da by calculation...
Recommended for supporting the growth of anchorage-dependent cells including human and mouse stromal cells, mesenchymal cells and cell lines....
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