Navigation Links
ATTO-TAG FQ Amine-Derivatization Kit from Molecular Probes (Invitrogen)

ProductsATTO-TAG FQ Amine-Derivatization Kit from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item ATTO-TAG FQ Amine-Derivatization Kit
Price 
Description Molecular Probes is pleased to offer the ATTO-TAG series of reagents for the ultrasensitive detection of primary amines. Developed by Novotny and collaborators at Indiana University, the ATTO-TAG reagents are similar to the important reagents o-phthaldialdehyde (OPA), naphthalene-2,3-dicarboxaldehyde (NDA) and anthracene-2,3-dicarboxaldehyde, all of which are also available in high purity form from Molecular Probes.

Principle:
ATTO-TAG CBQCA (3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde or CBQCA 1) and ATTO-TAG FQ (3-(2-furoyl) quinoline-2-carboxaldehyde 2) react specifically with primary amines to form conjugates that can be analyzed by electrophoretic or chromatographic methods. Unlike OPA and NDA, ATTO-TAG CBQCA will also react with hydrophilic peptides and amino sugars. The resulting products of CBQCA ? highly fluorescent 7-aza-1-cyano-5,6-benzisoindoles are maximally excited at 450nm or by the 442 nm spectral line of the He-Cd laser and have emission maxima at ~550 nm. The products of ATTO-TAG FQ are maximally excited at 480 nm or by the 488 nm line of the argon-ion laser and have emission maxima at ~590 nm. In capillary zone electrophoresis (CZE), the sensitivity of detection of ATTO-TAG CBQCA conjugates should be in the attomole range (10-18 moles). The high sensitivity, freedom from background and long-wavelength excitability make these potential reagents for research, diagnostic and forensic applications, including drug analysis. The ATTO-TAG reagents can, of course, be used in HPLC and other modes of chromatography with either absorbance or fluorescence detection. The principal limitation to obtaining ultrasensitive detection using chemical derivatization reagents, including the ATTO-TAG reagents, is that relatively high concentrations of the derivatizing reagent are required to obtain adequate kinetics and sufficient modification of the analyte. A recently described technique employs ATTOTAG FQ for the solid-phase derivatization of dilute solutions (10-8 M) of peptides that have been immobilized on Immobilon-CD membranes. This method permits the quantitative derivatization and analysis by capillary electrophoresis of only a few picomoles of the analyte.
Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
PO Box 22010
Eugene, OR 97402-0469

Call Molecular Probes (Invitrogen) to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-541-359-2658
Customer Service: 1-541-465-8300
Fax Number: 1-541-344-6504
Web Site: http://probes.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. ATTO-TAG CBQCA derivatization reagent (CBQCA; 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde) from Molecular Probes (Invitrogen)
2. ATTO-TAG CBQCA Amine-Derivatization Kit from Molecular Probes (Invitrogen)
3. Cleland’s Reagent, Molecular Biology Grade from Calbiochem
4. SYBR Green II RNA gel stain*10,000X concentrate in DMSO* from Molecular Probes (Invitrogen)
5. SYBR Green II RNA gel stain*10,000X concentrate in DMSO* from Molecular Probes (Invitrogen)
6. SYBR Green II RNA gel stain*10,000X concentrate in DMSO**special packaging* from Molecular Probes (Invitrogen)
7. AquaMax DW4 from Molecular Devices
8. GenePix Pro Microarray Image Analysis Software from Molecular Devices
9. adenosine 5-O-(3-thiotriphosphate), BODIPY FL conjugated/tagged from Molecular Probes (Invitrogen)
10. Acuity Enterprise Microarray Informatics Software from Molecular Devices
11. SlowFade Antifade Kit from Molecular Probes (Invitrogen)
... Dithiothreitol DTT White solid.. ... disulfides to thiols, so reaction is "driven" to ... not contain waxy surfactant found in many other ... pentobarbitol. Purity: ≥97% by titration. ...
... This kit is designed for easy transformation ... protocol allows for successful transformation simply by ... reagents with cultured yeast cells. No complicated ... are required. The kit is particularly well ...
... for easy transformation of the budding yeast, ... transformation simply by mixing a plasmid DNA ... cells. No complicated steps, such as centrifugation ... is particularly well suited for high-throughput transformation ...
... pro balance increases your lab productivity and allows ... and regulatory compliance. Move ahead beyond traditional paper-based ... data system. *Instruments supported: ... MX, UMX, AG, XS, XP, PR, SR, PG-S, ...
Biology Products:
(Date:7/27/2014)... and fungi can evade treatment by acquiring mutations in ... permanent mutations were once thought to be the only ... study has shown that microorganisms can use a temporary ... to gain the benefits of drug resistance without the ... a fungus called Mucor circinelloides, it is likely to ...
(Date:7/27/2014)... at Barts Cancer Institute have found that targeting a ... more effective, according to research published in Nature ... part of Queen Mary University of London, have found ... the body to repair itself after chemotherapy or radiotherapy, ... researchers removed FAK from blood vessels that grew in ...
(Date:7/27/2014)... 18,000 patients, scientists have identified more than two ... including six that had not been previously reported. ... was partially funded by the National Institutes of ... NIH laboratories. , "Unraveling the genetic underpinnings of ... involved in this complex disease, and hopefully, may ...
Breaking Biology News(10 mins):Scientists discover new, noncommittal mechanism of drug resistance 2Scientists discover new, noncommittal mechanism of drug resistance 3New drug target can break down cancer's barrier against treatment 2NIH scientists find 6 new genetic risk factors for Parkinson's 2
... laser laboratory operational later this year, he will use ... bonds like a stringed musical instrument. Tokmakoff, the ... at the University of Chicago in January to tackle ... vibrational spectroscopy methods that he has developed. "He ...
... Cognitive impairments are disabling for individuals with schizophrenia, ... affect a wide range of cognition, including memory, ... appear in the earliest stages of the disease ... Scientists are exploring a variety of strategies ...
... 2013 Shiner Beers, the popular Texas craft brew, will ... for Diabetes Research at the Translational Genomics Research Institute (TGen). ... kick off on Wednesday, March 13, at the Hotel San ... Conferences & Festivals. The noon-8 p.m. event, themed after ...
Cached Biology News:Watery research theme to flow through new Tokmakoff lab 2Watery research theme to flow through new Tokmakoff lab 3Tickling the brain with magnetic stimulation improves memory in schizophrenia 2Shiner Beers launches nationwide support of TGen diabetes studies 2
(Date:7/28/2014)... DC (PRWEB) July 28, 2014 It’s a ... GI Bill benefits to further their post-secondary education are failing ... a study released this year. And according to an analysis ... this shortfall is veteran students’ uninformed choice of college majors, ... The details of the study: Some 51.7 percent of post-9/11 ...
(Date:7/28/2014)... 28, 2014 Research and Markets has ... Market 2014-2018" report to their offering. ... is a technique used to determine the size of a ... is crucial to analyze the size of particles as it ... analysis is performed to determine the behavior and nature of ...
(Date:7/25/2014)... , July 25, 2014 Amgen (NASDAQ: ... Directors today declared a $0.61 per share dividend for ... paid on Sept. 5, 2014, to all stockholders of ... 14, 2014.  About Amgen Amgen is ... suffering from serious illnesses by discovering, developing, manufacturing and ...
(Date:7/25/2014)... According to a new market report ... Global Industry Analysis, Size, Share, Growth, Trends and Forecast, ... estimated at USD 161,056.5 million in 2014 and is ... 2014 to 2020, to reach an estimated value of ... market is witnessing a significant growth due to the ...
Breaking Biology Technology:Resume Place: To Choose the Best College Majors, Veterans Need Early Career Counseling, Data Shows 2Resume Place: To Choose the Best College Majors, Veterans Need Early Career Counseling, Data Shows 3Resume Place: To Choose the Best College Majors, Veterans Need Early Career Counseling, Data Shows 4Global Particle Size Analysis Market 2014-2018 2Amgen Announces 2014 Third Quarter Dividend 2Amgen Announces 2014 Third Quarter Dividend 3Biological Drugs Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 2Biological Drugs Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 3