Navigation Links
ATTO-TAG CBQCA Amine-Derivatization Kit from Molecular Probes (Invitrogen)

ProductsATTO-TAG CBQCA Amine-Derivatization Kit from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item ATTO-TAG CBQCA Amine-Derivatization Kit
Price 
Description Molecular Probes is pleased to offer the ATTO-TAG series of reagents for the ultrasensitive detection of primary amines. Developed by Novotny and collaborators at Indiana University, the ATTO-TAG reagents are similar to the important reagents o-phthaldialdehyde (OPA), naphthalene-2,3-dicarboxaldehyde (NDA) and anthracene-2,3-dicarboxaldehyde, all of which are also available in high purity form from Molecular Probes.

Principle:
ATTO-TAG CBQCA (3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde or CBQCA 1) and ATTO-TAG FQ (3-(2-furoyl) quinoline-2-carboxaldehyde 2) react specifically with primary amines to form conjugates that can be analyzed by electrophoretic or chromatographic methods. Unlike OPA and NDA, ATTO-TAG CBQCA will also react with hydrophilic peptides and amino sugars. The resulting products of CBQCA highly fluorescent 7-aza-1-cyano-5,6-benzisoindoles are maximally excited at 450nm or by the 442 nm spectral line of the He-Cd laser and have emission maxima at ~550 nm. The products of ATTO-TAG FQ are maximally excited at 480 nm or by the 488 nm line of the argon-ion laser and have emission maxima at ~590 nm. In capillary zone electrophoresis (CZE), the sensitivity of detection of ATTO-TAG CBQCA conjugates should be in the attomole range (10-18 moles). The high sensitivity, freedom from background and long-wavelength excitability make these potential reagents for research, diagnostic and forensic applications, including drug analysis. The ATTO-TAG reagents can, of course, be used in HPLC and other modes of chromatography with either absorbance or fluorescence detection. The principal limitation to obtaining ultrasensitive detection using chemical derivatization reagents, including the ATTO-TAG reagents, is that relatively high concentrations of the derivatizing reagent are required to obtain adequate kinetics and sufficient modification of the analyte. A recently described technique employs ATTOTAG FQ for the solid-phase derivatization of dilute solutions (10-8 M) of peptides that have been immobilized on Immobilon-CD membranes. This method permits the quantitative derivatization and analysis by capillary electrophoresis of only a few picomoles of the analyte.
Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
PO Box 22010
Eugene, OR 97402-0469

Call Molecular Probes (Invitrogen) to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-541-359-2658
Customer Service: 1-541-465-8300
Fax Number: 1-541-344-6504
Web Site: http://probes.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. ATTO-TAG FQ Amine-Derivatization Kit from Molecular Probes (Invitrogen)
2. ATTO-TAG CBQCA derivatization reagent (CBQCA; 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde) from Molecular Probes (Invitrogen)
3. Cleland’s Reagent, Molecular Biology Grade from Calbiochem
4. SYBR Green II RNA gel stain*10,000X concentrate in DMSO* from Molecular Probes (Invitrogen)
5. SYBR Green II RNA gel stain*10,000X concentrate in DMSO* from Molecular Probes (Invitrogen)
6. SYBR Green II RNA gel stain*10,000X concentrate in DMSO**special packaging* from Molecular Probes (Invitrogen)
7. AquaMax DW4 from Molecular Devices
8. GenePix Pro Microarray Image Analysis Software from Molecular Devices
9. adenosine 5-O-(3-thiotriphosphate), BODIPY FL conjugated/tagged from Molecular Probes (Invitrogen)
10. Acuity Enterprise Microarray Informatics Software from Molecular Devices
11. SlowFade Antifade Kit from Molecular Probes (Invitrogen)
... to 1 outflow Single-cell and patch delivery ... millisecond) solution change Very small dead volume ... construction 360, 250 and 100 micron delivery ... any micromanipulator Available in 4, 8, and ...
Sf21 cells derived from Spodoptera frugiperda ovarian cells are recommended for high-level production of recombinant proteins. Higher expression levels of some proteins are seen due to the larger ce...
... Signet offers the most comprehensive, cost-effective ... We have incorporated unprecedented quality into ... This includes proven detection chemistries and ... the TechMate protocol. It also includes ...
Functionally tested for consistent G-banding of chromosomes....
Biology Products:
(Date:6/15/2016)... 15, 2016 Transparency Market ... Recognition Market by Application Market - Global Industry Analysis Size ... to the report, the  global gesture recognition market ... and is estimated to grow at a CAGR ... 2024.  Increasing application of gesture recognition ...
(Date:6/9/2016)... June 9, 2016 Paris ... Teleste,s video security solution to ensure the safety of people ... during the major tournament Teleste, an international ... and services, announced today that its video security solution will ... to back up public safety across the country. The system ...
(Date:6/3/2016)... LONDON , June 3, 2016 /PRNewswire/ ... Transport Management) von Nepal ... ,Angebot und Lieferung hochsicherer geprägter Kennzeichen, einschließlich ... weltweit führend in der Produktion und Implementierung ... an der Ausschreibung im Januar teilgenommen, aber ...
Breaking Biology News(10 mins):
(Date:12/6/2016)... ... December 06, 2016 , ... The Osteoarthritis Research Society International ... Administration (FDA) to consider OA as a serious disease. As an organization of ... population of OA patients, many of whom may experience progressive disability and decreased ...
(Date:12/6/2016)... ... December 06, 2016 , ... ... Partners ("GPP") portfolio company, today announced it has acquired the assets of ... of Chiltern International and focuses on clinical trial drug packaging, labeling, storage, ...
(Date:12/6/2016)... 2016  SRI International has been awarded a ... National Institutes of Health,s National Institute of Allergy ... (NIAID-DAIDS) to support the manufacturing and characterization of ... Under the seven-year contract, SRI will provide a ... candidate HIV-prevention products that emerge from investigator-initiated studies ...
(Date:12/5/2016)... , Dec. 5, 2016 Axovant Sciences Ltd. ... company focused on the treatment of dementia, today announced ... the treatment of Alzheimer,s disease will be presented at ... on Friday, December 9, 2016 in San ... results of both simple and complex measures of activities ...
Breaking Biology Technology: