Navigation Links
ATTO-TAG CBQCA Amine-Derivatization Kit from Molecular Probes (Invitrogen)

ProductsATTO-TAG CBQCA Amine-Derivatization Kit from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item ATTO-TAG CBQCA Amine-Derivatization Kit
Price 
Description Molecular Probes is pleased to offer the ATTO-TAG series of reagents for the ultrasensitive detection of primary amines. Developed by Novotny and collaborators at Indiana University, the ATTO-TAG reagents are similar to the important reagents o-phthaldialdehyde (OPA), naphthalene-2,3-dicarboxaldehyde (NDA) and anthracene-2,3-dicarboxaldehyde, all of which are also available in high purity form from Molecular Probes.

Principle:
ATTO-TAG CBQCA (3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde or CBQCA 1) and ATTO-TAG FQ (3-(2-furoyl) quinoline-2-carboxaldehyde 2) react specifically with primary amines to form conjugates that can be analyzed by electrophoretic or chromatographic methods. Unlike OPA and NDA, ATTO-TAG CBQCA will also react with hydrophilic peptides and amino sugars. The resulting products of CBQCA highly fluorescent 7-aza-1-cyano-5,6-benzisoindoles are maximally excited at 450nm or by the 442 nm spectral line of the He-Cd laser and have emission maxima at ~550 nm. The products of ATTO-TAG FQ are maximally excited at 480 nm or by the 488 nm line of the argon-ion laser and have emission maxima at ~590 nm. In capillary zone electrophoresis (CZE), the sensitivity of detection of ATTO-TAG CBQCA conjugates should be in the attomole range (10-18 moles). The high sensitivity, freedom from background and long-wavelength excitability make these potential reagents for research, diagnostic and forensic applications, including drug analysis. The ATTO-TAG reagents can, of course, be used in HPLC and other modes of chromatography with either absorbance or fluorescence detection. The principal limitation to obtaining ultrasensitive detection using chemical derivatization reagents, including the ATTO-TAG reagents, is that relatively high concentrations of the derivatizing reagent are required to obtain adequate kinetics and sufficient modification of the analyte. A recently described technique employs ATTOTAG FQ for the solid-phase derivatization of dilute solutions (10-8 M) of peptides that have been immobilized on Immobilon-CD membranes. This method permits the quantitative derivatization and analysis by capillary electrophoresis of only a few picomoles of the analyte.
Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
PO Box 22010
Eugene, OR 97402-0469

Call Molecular Probes (Invitrogen) to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-541-359-2658
Customer Service: 1-541-465-8300
Fax Number: 1-541-344-6504
Web Site: http://probes.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. ATTO-TAG FQ Amine-Derivatization Kit from Molecular Probes (Invitrogen)
2. ATTO-TAG CBQCA derivatization reagent (CBQCA; 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde) from Molecular Probes (Invitrogen)
3. Cleland’s Reagent, Molecular Biology Grade from Calbiochem
4. SYBR Green II RNA gel stain*10,000X concentrate in DMSO* from Molecular Probes (Invitrogen)
5. SYBR Green II RNA gel stain*10,000X concentrate in DMSO* from Molecular Probes (Invitrogen)
6. SYBR Green II RNA gel stain*10,000X concentrate in DMSO**special packaging* from Molecular Probes (Invitrogen)
7. AquaMax DW4 from Molecular Devices
8. GenePix Pro Microarray Image Analysis Software from Molecular Devices
9. adenosine 5-O-(3-thiotriphosphate), BODIPY FL conjugated/tagged from Molecular Probes (Invitrogen)
10. Acuity Enterprise Microarray Informatics Software from Molecular Devices
11. SlowFade Antifade Kit from Molecular Probes (Invitrogen)
... is designed for easy transformation of the ... for successful transformation simply by mixing a ... cultured yeast cells. No complicated steps, such ... The kit is particularly well suited for ...
... Microplate Handler is a high-capacity, plug-and-play ... BioTeks washers, readers and liquid handling ... ultimate flexibility and intuitive and powerful ... to single devices or linking several ...
... It offers a wide array of features for weighing ... supported: Control of ... SR, PG-S, PG, SG, AT, MT, UMT, AB-S, PB-S, ... User guidance on the balance display *Execute ...
XPLAC (S-17)...
Biology Products:
(Date:3/14/2016)... , March 14, 2016 NXTD ) ... mobile commerce market, announces the airing of a new series ... the week of March 21 st .  The commercials will ... its popular Squawk on the Street show. --> ... the growing mobile commerce market, announces the airing of a ...
(Date:3/10/2016)... Pa. , March 10, 2016   Unisys Corporation ... Customs and Border Protection (CBP) is testing its biometric ... San Diego to help identify certain non-U.S. ... . The test, designed to help determine the efficiency ... environment, began in February and will run until May 2016. ...
(Date:3/3/2016)... -- FlexTech, a SEMI Strategic Association Partner, awarded five FLEXI ... Leadership in Education, and, in a category new this ... year of the FLEXI Awards and the winners join ... past years . Judging was done on a set ... by a panel of non-affiliated, independent, industry experts. ...
Breaking Biology News(10 mins):
(Date:5/5/2016)... ... May 05, 2016 , ... ProMIS Neurosciences (“ProMIS” or the ... targets, it has identified a fourth in a series of novel potential therapeutic ... progression of Alzheimer’s disease (AD). , “This discovery is an important ...
(Date:5/4/2016)... ... 04, 2016 , ... The Children’s Tumor Foundation announced its annual month-long campaign ... on nerves throughout the body. It affects 1 in 3,000 people of all populations; ... the month of May, as well as online activities, Neurofibromatosis Awareness Month and “I ...
(Date:5/3/2016)... Francisco, CA (PRWEB) , ... May 03, 2016 ... ... Morf Playbook™ enterprise talent development, skill-building and compliance training platform on mobile ... training course: Overview of Regulatory Requirements for Medical Devices. The course is ...
(Date:5/3/2016)... (PRWEB) , ... May 03, 2016 , ... ... NeuroTech NYC , the sensor and data driven conferences, will take place on June 7-8, ... UnitedHealth's Vidya Raman-Tangella on incorporating technology -- including AR/VR, machine learning, ...
Breaking Biology Technology: