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ABsolute QRT-PCR SYBR Green Fluorescein Mix from ABgene

ProductsABsolute QRT-PCR SYBR Green Fluorescein Mix from ABgene
Company ABgene
Item ABsolute QRT-PCR SYBR Green Fluorescein Mix
Description ABsolute QRT-PCR Mixes are formulated for use in one-step QRT-PCR reactions, with gene-specific primers and SYBR Green. One-step QRT-PCR mixes offer a quick and simple method for analysing RNA. The pipetting steps are minimised, making the protocol easy to follow and automation possible. The 2x ABsolute QRT-PCR Mix is supplied with a patented blend of RTases combining the advantages of AMV and MMuLV RTases to give unparalleled performance. The mix can be used at elevated temperatures, avoiding problems caused by secondary structure in the RNA template. In addition the QRTase Blend has greater dynamic range than either AMV or MMuLV. This leads to greater accuracy in QRT-PCR experiments. Unique QRTase Enhancer - Provided to reduce problems associated with DNA contamination. ABsolute QRTase Blend - ABsolute QRT-PCR kits contain a unique blend of MMuLV and AMV RTases in a buffer designed for synergy with subsequent QPCR reactions. This allows detection of rare templates. The RNase H activity facilitates the freeing of priming sites during the QPCR stage of the reaction. The AMV component allows reverse transcription to be carried out at up to 57C when secondary structure is problematic. A RNase inhibitor is included in the blend to prevent RNA degradation from RNase A. Optimised Buffer - ABsolute QRT-PCR Mixes are based on a balanced buffer to maximise performance of both the QRTase Blend and Thermo-Start Polymerase. SYBR Green I is also included in the buffer at an optimum concentration to facilitate detection of low abundance templates. Maximum Specificity - Thermo-Start DNA Polymerase remains completely inactive during the reverse transcription stage of QRT-PCR. Enzyme is fully activated by a 15minute activation at 95C. Simple protocol Easily adapted for automation This mix with Fluorescein included is ideal for use in Bio-Rad instruments - other formats available on request Applications: real-time QRT-PCR SYBR Green I assays, end-point analysis

We are part of Thermo Fisher Scientific, the world leader in serving science. Thermo Fisher Scientific enables customers to make the world healthier, cleaner and safer by providing analytical instruments, equipment, reagents and consumables, software and services for research, analysis, discovery and diagnostics. With annual sales of more than $9 billion, Thermo Fisher Scientific has 30,000 employees and serves more than 350,000 customers in pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies as well as environmental, industrial quality and process control settings.

  • ABgene produces specialist products for nucleic acid amplification and biostorage. ABgenes focus is on rapidly responding to the continually developing markets of drug discovery, genomic and forensic research and biological archiving.
  • ABgenes product lines include PCR and QPCR reagents, molecular biology consumables and instrumentation designed to complement each other for increased productivity.
Info ABgeneABgene
ABgene House
Blenheim Road
Surrey KT19 9AP
Customer Service: +44 (0)1372 723456
Fax Number: +44 (0)1372 741414
Web Site:
NOTE: Price information is approximate list price and actual prices may vary.

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