Navigation Links
ABsolute QRT-PCR SYBR Green Fluorescein Mix from ABgene

ProductsABsolute QRT-PCR SYBR Green Fluorescein Mix from ABgene
Company ABgene
Item ABsolute QRT-PCR SYBR Green Fluorescein Mix
Price 
Description ABsolute QRT-PCR Mixes are formulated for use in one-step QRT-PCR reactions, with gene-specific primers and SYBR Green. One-step QRT-PCR mixes offer a quick and simple method for analysing RNA. The pipetting steps are minimised, making the protocol easy to follow and automation possible. The 2x ABsolute QRT-PCR Mix is supplied with a patented blend of RTases combining the advantages of AMV and MMuLV RTases to give unparalleled performance. The mix can be used at elevated temperatures, avoiding problems caused by secondary structure in the RNA template. In addition the QRTase Blend has greater dynamic range than either AMV or MMuLV. This leads to greater accuracy in QRT-PCR experiments. Unique QRTase Enhancer - Provided to reduce problems associated with DNA contamination. ABsolute QRTase Blend - ABsolute QRT-PCR kits contain a unique blend of MMuLV and AMV RTases in a buffer designed for synergy with subsequent QPCR reactions. This allows detection of rare templates. The RNase H activity facilitates the freeing of priming sites during the QPCR stage of the reaction. The AMV component allows reverse transcription to be carried out at up to 57C when secondary structure is problematic. A RNase inhibitor is included in the blend to prevent RNA degradation from RNase A. Optimised Buffer - ABsolute QRT-PCR Mixes are based on a balanced buffer to maximise performance of both the QRTase Blend and Thermo-Start Polymerase. SYBR Green I is also included in the buffer at an optimum concentration to facilitate detection of low abundance templates. Maximum Specificity - Thermo-Start DNA Polymerase remains completely inactive during the reverse transcription stage of QRT-PCR. Enzyme is fully activated by a 15minute activation at 95C. Simple protocol Easily adapted for automation This mix with Fluorescein included is ideal for use in Bio-Rad instruments - other formats available on request Applications: real-time QRT-PCR SYBR Green I assays, end-point analysis

We are part of Thermo Fisher Scientific, the world leader in serving science. Thermo Fisher Scientific enables customers to make the world healthier, cleaner and safer by providing analytical instruments, equipment, reagents and consumables, software and services for research, analysis, discovery and diagnostics. With annual sales of more than $9 billion, Thermo Fisher Scientific has 30,000 employees and serves more than 350,000 customers in pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies as well as environmental, industrial quality and process control settings.

  • ABgene produces specialist products for nucleic acid amplification and biostorage. ABgenes focus is on rapidly responding to the continually developing markets of drug discovery, genomic and forensic research and biological archiving.
  • ABgenes product lines include PCR and QPCR reagents, molecular biology consumables and instrumentation designed to complement each other for increased productivity.
Info ABgeneABgene
ABgene House
Blenheim Road
Epsom
Surrey KT19 9AP
UK
Customer Service: +44 (0)1372 723456
Fax Number: +44 (0)1372 741414
Web Site: http://www.abgene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. ABsolute QPCR SYBR Green ROX Mix from ABgene
2. SYBR Green II RNA gel stain*10,000X concentrate in DMSO* from Molecular Probes (Invitrogen)
3. SYBR Green II RNA gel stain*10,000X concentrate in DMSO* from Molecular Probes (Invitrogen)
4. SYBR Green II RNA gel stain*10,000X concentrate in DMSO**special packaging* from Molecular Probes (Invitrogen)
5. SYBR Green I nucleic acid gel stain*10,000X concentrate in DMSO* from Molecular Probes (Invitrogen)
6. SYBR Green I nucleic acid gel stain*10,000X concentrate in DMSO**special packaging* from Molecular Probes (Invitrogen)
7. SYBR Green I nucleic acid gel stain from Molecular Probes (Invitrogen)
8. PR Competitor Assay Green from Invitrogen
9. PPARGamma-Competitor Assay Kit Green from Invitrogen
10. Lysozyme Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
11. Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
... Kits provide rapid and efficient removal of ... and salts from PCR products (Figure 1). ... two Binding Buffer options for fragments >100 ... performed in either single column or 96-well ...
... Sequencing allow 5-minute cloning and subsequent sequencing of ... with primer sites located as close as 33 ... This minimizes the amount of vector DNA that ... of your insert.vectors are available. The pCR4-TOPO vector ...
Zebrafish Vitellogenin ELISA System, 96 wells. ELISARange: 0.5-100 ng/ml.Sensitivity: 0.3 ng/ml.Sample volume: 100 ul.Suitable for use with Zebrafish plasma samples.3-h protocol.Store at 2-8 C. Categ...
... Normal;heading 1;heading ... types and experimental ... for standard transfection ... transduction systems. ViraPower ...
Biology Products:
(Date:3/14/2016)... Florida , March 14, 2016 ... the growing mobile commerce market, announces the airing of a ... channels starting the week of March 21 st .  The ... CNBC, including its popular Squawk on the Street show. ... focused on the growing mobile commerce market, announces the airing ...
(Date:3/11/2016)... March 11, 2016 --> ... research report "Image Recognition Market by Technology (Pattern Recognition), ... Advertising), by Deployment Type (On-Premises and Cloud), by Industry ... published by MarketsandMarkets, the global market is expected to ... 29.98 Billion by 2020, at a CAGR of 19.1%. ...
(Date:3/9/2016)... 2016 This BCC Research report provides an ... RNA Sequencing (RNA Seq) market for the years 2015, ... and reagents, data analysis, and services. Use ... RNA-Sequencing market such as RNA-Sequencing tools and reagents, RNA-Sequencing ... affecting each segment and forecast their market growth, future ...
Breaking Biology News(10 mins):
(Date:4/27/2016)... ... April 27, 2016 , ... The Pittcon Organizing Committee is ... Chuck has been a volunteer member of Committee since 1987. Since then, he has ... directors and treasurer and was chairman for both the program and exposition committees. In ...
(Date:4/27/2016)... ... April 27, 2016 , ... ... has joined the company as an Expert Consultant. Mr. Clark was formerly ... and managing the development of small molecule monographs based on analytical methods. ...
(Date:4/27/2016)... 27, 2016 MedDay, a biotechnology company ... the appointment of Catherine Moukheibir as Chairman of its Board ... Jean Jacques Garaud , who contributed to the rapid ... immediately. Catherine started her career in strategy consulting ... London .  She held C-Suite level roles ...
(Date:4/26/2016)... ... April 26, 2016 , ... ... systems, announces the latest technology innovation for its Volume Pattern Generator (VPG) line ... for production of advanced photomasks as well as a solution for mid volume ...
Breaking Biology Technology: