Navigation Links
ABsolute QRT-PCR SYBR Green Fluorescein Mix from ABgene

ProductsABsolute QRT-PCR SYBR Green Fluorescein Mix from ABgene
Company ABgene
Item ABsolute QRT-PCR SYBR Green Fluorescein Mix
Price 
Description ABsolute QRT-PCR Mixes are formulated for use in one-step QRT-PCR reactions, with gene-specific primers and SYBR Green. One-step QRT-PCR mixes offer a quick and simple method for analysing RNA. The pipetting steps are minimised, making the protocol easy to follow and automation possible. The 2x ABsolute QRT-PCR Mix is supplied with a patented blend of RTases combining the advantages of AMV and MMuLV RTases to give unparalleled performance. The mix can be used at elevated temperatures, avoiding problems caused by secondary structure in the RNA template. In addition the QRTase Blend has greater dynamic range than either AMV or MMuLV. This leads to greater accuracy in QRT-PCR experiments. Unique QRTase Enhancer - Provided to reduce problems associated with DNA contamination. ABsolute QRTase Blend - ABsolute QRT-PCR kits contain a unique blend of MMuLV and AMV RTases in a buffer designed for synergy with subsequent QPCR reactions. This allows detection of rare templates. The RNase H activity facilitates the freeing of priming sites during the QPCR stage of the reaction. The AMV component allows reverse transcription to be carried out at up to 57C when secondary structure is problematic. A RNase inhibitor is included in the blend to prevent RNA degradation from RNase A. Optimised Buffer - ABsolute QRT-PCR Mixes are based on a balanced buffer to maximise performance of both the QRTase Blend and Thermo-Start Polymerase. SYBR Green I is also included in the buffer at an optimum concentration to facilitate detection of low abundance templates. Maximum Specificity - Thermo-Start DNA Polymerase remains completely inactive during the reverse transcription stage of QRT-PCR. Enzyme is fully activated by a 15minute activation at 95C. Simple protocol Easily adapted for automation This mix with Fluorescein included is ideal for use in Bio-Rad instruments - other formats available on request Applications: real-time QRT-PCR SYBR Green I assays, end-point analysis

We are part of Thermo Fisher Scientific, the world leader in serving science. Thermo Fisher Scientific enables customers to make the world healthier, cleaner and safer by providing analytical instruments, equipment, reagents and consumables, software and services for research, analysis, discovery and diagnostics. With annual sales of more than $9 billion, Thermo Fisher Scientific has 30,000 employees and serves more than 350,000 customers in pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies as well as environmental, industrial quality and process control settings.

  • ABgene produces specialist products for nucleic acid amplification and biostorage. ABgenes focus is on rapidly responding to the continually developing markets of drug discovery, genomic and forensic research and biological archiving.
  • ABgenes product lines include PCR and QPCR reagents, molecular biology consumables and instrumentation designed to complement each other for increased productivity.
Info ABgeneABgene
ABgene House
Blenheim Road
Epsom
Surrey KT19 9AP
UK
Customer Service: +44 (0)1372 723456
Fax Number: +44 (0)1372 741414
Web Site: http://www.abgene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. ABsolute QPCR SYBR Green ROX Mix from ABgene
2. SYBR Green II RNA gel stain*10,000X concentrate in DMSO* from Molecular Probes (Invitrogen)
3. SYBR Green II RNA gel stain*10,000X concentrate in DMSO* from Molecular Probes (Invitrogen)
4. SYBR Green II RNA gel stain*10,000X concentrate in DMSO**special packaging* from Molecular Probes (Invitrogen)
5. SYBR Green I nucleic acid gel stain*10,000X concentrate in DMSO* from Molecular Probes (Invitrogen)
6. SYBR Green I nucleic acid gel stain*10,000X concentrate in DMSO**special packaging* from Molecular Probes (Invitrogen)
7. SYBR Green I nucleic acid gel stain from Molecular Probes (Invitrogen)
8. PR Competitor Assay Green from Invitrogen
9. PPARGamma-Competitor Assay Kit Green from Invitrogen
10. Lysozyme Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
11. Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
... stimulating factors for assays of mouse hematopoietic ... fetal liver. Suitable for the growth ... when added to methylcellulose at a final ... Will also support growth of mouse ...
Mouse monoclonal antibody to FMN2 - formin 2...
...
Anti-Human Complement Component C4c, IgY fraction (Polyclonal) (chicken IgY)...
Biology Products:
(Date:3/27/2017)... , March 27, 2017  Catholic Health ... and Management Systems Society (HIMSS) Analytics for achieving ... Adoption Model sm . In addition, CHS previously ... U.S. hospitals using an electronic medical record (EMR). ... its high level of EMR usage in an ...
(Date:3/24/2017)... , Mar 24, 2017 Research and Markets ... System Market Analysis & Trends - Industry Forecast to 2025" ... ... grow at a CAGR of around 15.1% over the next decade ... industry report analyzes the market estimates and forecasts for all the ...
(Date:3/23/2017)... Research and Markets has announced the addition ... - Industry Forecast to 2025" report to their offering. ... The Global Vehicle ... around 8.8% over the next decade to reach approximately $14.21 billion ... estimates and forecasts for all the given segments on global as ...
Breaking Biology News(10 mins):
(Date:9/19/2017)... ... 19, 2017 , ... Molecular Devices, LLC, a leader in ... launch the CloneSelect™ Single-Cell Printer™ in North America. This novel system utilizes ... visual documentation of monoclonality for use in cell line development. , Clonal ...
(Date:9/18/2017)... ... September 18, 2017 , ... Transportable biomass conversion facilities ... torrefied wood is the topic of a September 27 webinar hosted ... viability of transportable biomass conversion facilities for producing biochar, briquettes, and torrefied wood, ...
(Date:9/18/2017)... ... September 18, 2017 , ... ... optimization firm for the life sciences and healthcare industries, announces Bryan Coddington ... , What: Digital Transformation in Medical Device – The Journey to FDA Compliant ...
(Date:9/14/2017)... ... September 14, 2017 , ... ... formally introduces its flexible scientist program (FSP)-- a flexible business approach similar ... FSP, which combines SSCI’s extensive project-based analytical and solid-state chemistry services and ...
Breaking Biology Technology: