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4',6-Diamidino-2-phenylindole dihydrochloride (DAPI) from Sigma-Aldrich

Products4',6-Diamidino-2-phenylindole dihydrochloride (DAPI) from Sigma-Aldrich
Company Sigma-Aldrich
Item 4',6-Diamidino-2-phenylindole dihydrochloride (DAPI)
Description Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Info Sigma-AldrichSigma-Aldrich
Sigma-Aldrich Corp.
St. Louis, MO, USA
Customer Service: 800-325-3010
Tech Support: 800-325-5832
Fax Number: 314-771-5757
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NOTE: Price information is approximate list price and actual prices may vary.

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