Navigation Links
20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON

Products20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON
Company CHEMICON
Item 20S Proteasome Fluorometric (AMC) Assay Kit
Price $325.00
Description The ubiquitin-proteasome pathway is the major proteolytic system in the cytosol of eukaryotic cells, where it catalyzes the selective degradation of short-lived regulatory proteins and the rapid elimination of proteins with abnormal conformation (Hershko & Ciechanover 1998; Hochstrasser 1996). The critical protease in this pathway is the 26S proteasome, an ATP-dependent proteolytic complex, which is formed by the association of the barrel-shaped 20S proteasome (700-kDa) and two 19S (700-kDa) regulatory complexes (Baumeister et al. 1998; Coux et al. 1996). The 20S Proteasome, catalytic core of the proteasome complex, is responsible for the breakdown of key proteins involved with apoptosis, DNA repair, endocytosis, and cell cycle control (Coux et al. 1996; Hoffman & Rechsteiner 1996; Hochstrasser 1995). The CHEMICON Proteasome Activity Assay Kit provides a quick, efficient and sensitive system for evaluation of proteasome activity in cell lysates or inhibitor screening. Test Principle: Chemicons Proteasome Activity Assay Kit provides a simple and convenient means for assaying proteasome activity that recognize the substrate LLVY (Meng et al. 1999). The assay is based on detection of the fluorophore 7-Amino-4-methylcoumarin (AMC) after cleavage from the labeled substrate LLVY-AMC. The free AMC fluorescence can be quantified using a 380/460 nm filter set in a fluorometer. A proteasome inhibitor, Lactacystin, is included as a test inhibitor for screening purpose. Lactacystin is a microbial natural product and the most specific and potent inhibitor of proteasomes currently known (Fenteany & Schreiber 1998). Application: The Proteasome Activity Assay Kit is a relatively quick method for detection of intracellular proteasome activity. Testing of purified proteasome enzyme, in vitro inhibitor screening and the study of proteasome regulation can be performed with this assay.
Info CHEMICONCHEMICON
CHEMICON International, Inc.
28820 Single Oak Drive
Temecula, CA 92590
Customer Service: 800-437-7500
Tech Support: 800-437-7500
Fax Number: 800-437-7502
Web Site: http://www.chemicon.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. 26S Proteasome Fraction Extract from GeneTex
2. Ubiquitin/Proteasome Fraction II Extract from GeneTex
3. 26S Proteasome Fraction Extract from GeneTex
4. Mouse Anti-20S Proteasome alpha1, 2, 3, 5, 6, & 7-Subunits Monoclonal Antibody, Unconjugated, Clone MCP231 from Calbiochem
5. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
6. Cell invasion Fluorometric (green) Assay Kit, InnoCyte™ from Calbiochem
7. Telomerase Fluorometric Assay Kit, TRAPeze from CHEMICON
8. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
9. TACE Fluorometric (MCA)) Assay Kit, InnoZyme™ from Calbiochem
10. Lysozyme Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
11. Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
Sequencing and primer design rolled into one. Just give us your clone and a reference sequence, and we'll do the rest. Single-strand or double-strand coverage. Guaranteed results....
...
Anti-Wnt-2 Polyclonal Antibody Description: 100 g purified goat polyclonal antibody. Reacts with human/mouse/rat. Tested in Western blot/IP/IHC. Research Focus: signal transduction Storage...
... custom microplate coating using advanced automated high throughput microplate processing ... We can also accommodate virtually any lot size of the ... , 8 and 12 well ... 384 well microplate, ...
Biology Products:
(Date:5/29/2015)... May 28, 2015 Research and Markets ... the "Facial Recognition Market by Solution, by ... Forecast to 2020" report to their offering. ... facial recognition technology become more efficient and cost ... traction and investment from the commercial sector. The ...
(Date:5/22/2015)... 2015  NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or ... ending its promotional pricing and making the Wocket generally available ... Savin , Head of Marketing said, "We,ve extended the opportunity ... end of May. To get your wocket at this special ... the store on June 1, you,ll receive a unique code ...
(Date:5/20/2015)... OXFORD, Conn. , May 20, 2015 ... "Company") announces that its wholly owned subsidiary, 3D-ID LLC, ... contractor under Team Battelle for the biometrics technology portion ... Unrestricted Suite Contract from the Department of the Army. ... facial recognition products developed for government, law enforcement and ...
Breaking Biology News(10 mins):Global advanced Facial Recognition Market Expected to Grow From $2.77 Billion in 2015 to $6.19 Billion in 2020, at an Estimated CAGR of 17.4% 2NXT-ID, Inc Announces Retail Price For Wocket Smart Wallet And Extends Discounted Promotional Price Till End Of May 2NXT-ID, Inc Announces Retail Price For Wocket Smart Wallet And Extends Discounted Promotional Price Till End Of May 3NXT-ID, Inc Announces Participation as Member of Team Battelle for TIES Contract Award 2NXT-ID, Inc Announces Participation as Member of Team Battelle for TIES Contract Award 3
... For his creative work in evolutionary systems biology, Jeff ... Technology, is The Pew Charitable Trusts, biomedical researcher of the month. Gore ... the goal of understanding how pathogens become resistant to drugs. ... Inspired ...
... is available in German . ... previously assumed. More precise tests may allow allergy sufferers more freedom ... studies carried out by the Helmholtz Centre for Environmental Research (UFZ), ... The researchers examined patients with a Nile perch allergy. Being allergic ...
... on the biochemical makeup of small interfering RNA ... used in research to help scientists better understand ... accessible to researchers worldwide increases the potential of ... National Center for Advancing Translational Sciences (NCATS) collaborated ...
Cached Biology News:Each food fish can cause specific allergies 2Each food fish can cause specific allergies 3Gene-silencing data now publicly available to help scientists better understand disease 2Gene-silencing data now publicly available to help scientists better understand disease 3
(Date:6/3/2015)... June 3, 2015  HireLifeScience.com, the premier career ... host its 6 th annual career fair ... Raritan Center in Edison, NJ ... Novo Nordisk, Covance, Actavis and AstraZeneca.  Additional Pharmaceutical, ... exhibiting are Johnson & Johnson, MedImmune, DSM Nutritional ...
(Date:6/3/2015)... York and London (PRWEB) June 03, 2015 ... (NCSA) recently set a new world record by ... cores on the Blue Waters supercomputer. , ... used across many industries to stimulate innovation and ... Private Sector Program and Blue Waters manufacturer Cray ...
(Date:6/3/2015)... June 03, 2015 From smart street ... important part in enabling success in dealing with important ... technology leaders helped bring focus to the role and ... Photonics21 annual meeting last week in Brussels. ... event, held 28 and 29 May at the Hotel ...
(Date:6/2/2015)... 2015 Research and Markets ( http://www.researchandmarkets.com/research/6wjpd6/bioburden_testing ) ... Market by Product , Test, Application & End User ... The bioburden testing market is expected to ... 2014, growing at a CAGR of 9.8% from 2014 ... microbial limit testing market is segmented into consumables and ...
Breaking Biology Technology:AstraZeneca Added as Sponsor for Industry Leading Life Science Hiring Event 2NCSA Scales CD-adapco’s STAR-CCM+ to New World Record of 102,000 Cores on Blue Waters 2NCSA Scales CD-adapco’s STAR-CCM+ to New World Record of 102,000 Cores on Blue Waters 3Photonics21 Annual Meeting Focuses on Solutions for ‘Societal Challenges’ 2Photonics21 Annual Meeting Focuses on Solutions for ‘Societal Challenges’ 3Bioburden Testing Market 2015 - Global Forecasts to 2019 2