Navigation Links
20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON

Products20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON
Company CHEMICON
Item 20S Proteasome Fluorometric (AMC) Assay Kit
Price $325.00
Description The ubiquitin-proteasome pathway is the major proteolytic system in the cytosol of eukaryotic cells, where it catalyzes the selective degradation of short-lived regulatory proteins and the rapid elimination of proteins with abnormal conformation (Hershko & Ciechanover 1998; Hochstrasser 1996). The critical protease in this pathway is the 26S proteasome, an ATP-dependent proteolytic complex, which is formed by the association of the barrel-shaped 20S proteasome (700-kDa) and two 19S (700-kDa) regulatory complexes (Baumeister et al. 1998; Coux et al. 1996). The 20S Proteasome, catalytic core of the proteasome complex, is responsible for the breakdown of key proteins involved with apoptosis, DNA repair, endocytosis, and cell cycle control (Coux et al. 1996; Hoffman & Rechsteiner 1996; Hochstrasser 1995). The CHEMICON Proteasome Activity Assay Kit provides a quick, efficient and sensitive system for evaluation of proteasome activity in cell lysates or inhibitor screening. Test Principle: Chemicons Proteasome Activity Assay Kit provides a simple and convenient means for assaying proteasome activity that recognize the substrate LLVY (Meng et al. 1999). The assay is based on detection of the fluorophore 7-Amino-4-methylcoumarin (AMC) after cleavage from the labeled substrate LLVY-AMC. The free AMC fluorescence can be quantified using a 380/460 nm filter set in a fluorometer. A proteasome inhibitor, Lactacystin, is included as a test inhibitor for screening purpose. Lactacystin is a microbial natural product and the most specific and potent inhibitor of proteasomes currently known (Fenteany & Schreiber 1998). Application: The Proteasome Activity Assay Kit is a relatively quick method for detection of intracellular proteasome activity. Testing of purified proteasome enzyme, in vitro inhibitor screening and the study of proteasome regulation can be performed with this assay.
Info CHEMICONCHEMICON
CHEMICON International, Inc.
28820 Single Oak Drive
Temecula, CA 92590
Customer Service: 800-437-7500
Tech Support: 800-437-7500
Fax Number: 800-437-7502
Web Site: http://www.chemicon.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. 26S Proteasome Fraction Extract from GeneTex
2. Ubiquitin/Proteasome Fraction II Extract from GeneTex
3. 26S Proteasome Fraction Extract from GeneTex
4. Mouse Anti-20S Proteasome alpha1, 2, 3, 5, 6, & 7-Subunits Monoclonal Antibody, Unconjugated, Clone MCP231 from Calbiochem
5. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
6. Cell invasion Fluorometric (green) Assay Kit, InnoCyte™ from Calbiochem
7. Telomerase Fluorometric Assay Kit, TRAPeze from CHEMICON
8. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
9. TACE Fluorometric (MCA)) Assay Kit, InnoZyme™ from Calbiochem
10. Lysozyme Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
11. Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
... is a new product number, ... product number. If showing no ... the old Sigma-Aldrich number (Z37,208-0) ... assistance. Mfr Desig: Corning ...
... Anti-YAP Polyclonal Antibody ... purified rabbit polyclonal antibody. Detects 70 ... in Western blotting and immunohistochemistry. ... proliferation Storage: ...
... Introducing SilverSNAP Stain for Mass Spectrometry ... proteome, mass spectrometry (MS) has emerged as ... Silver staining of 2-D gels is now ... of procedures that leads ultimately to identification ...
... FailSafe™ PCR System is the only PCR ... time and every time. It provides accuracy, ... the source or the property of the ... PCR Enzyme Mix, a unique blend of ...
Biology Products:
(Date:7/20/2017)... 2017 Delta (NYSE: DAL ) customers now can ... at Reagan Washington National Airport (DCA). ... Delta launches biometrics to board aircraft at Reagan ... Delta,s biometric boarding pass experience that ... now integrated into the boarding process to allow eligible Delta SkyMiles Members ...
(Date:5/23/2017)... -- Hunova, the first robotic gym for the rehabilitation and functional motor sense ... Genoa, Italy . The first 30 robots will be available ... USA . The technology was developed and patented at the IIT ... Movendo Technology thanks to a 10 million euro investment from entrepreneur Sergio ... ...
(Date:4/24/2017)... 24, 2017 Janice Kephart , ... Identity Strategy Partners, LLP (IdSP) , today issues ... President Trump,s March 6, 2017 Executive Order: ... vetting can be instilled with greater confidence, enabling ... all refugee applications are suspended by until at ...
Breaking Biology News(10 mins):
(Date:9/19/2017)... ... September 19, 2017 , ... ... (NYS DFS) cybersecurity regulations have transitioned into full force and effect. ... in the state (“Covered Entities”) to conduct an annual, professional, comprehensive cybersecurity ...
(Date:9/18/2017)... --  Montrium , a growing leader in Electronic Trial Master ... groundbreaking non-profit research organization, Multidisciplinary Association for Psychedelic Studies (MAPS). ... MAPS Public Benefit ... ... to MDMA for the treatment of Posttraumatic Stress Disorder (PTSD). MAPS ...
(Date:9/14/2017)... Lafayette, Ind (PRWEB) , ... ... ... of AMRI’s Global Analytical Services, formally introduces its flexible scientist program (FSP)-- ... offers volume-based, preferred-rate pricing. The FSP, which combines SSCI’s extensive project-based analytical ...
(Date:9/14/2017)... ... September 14, 2017 , ... AxioMed is pleased ... Australia. Dr. Steven Yang completed the procedure on a 35-year-old female patient suffering ... lumbar disc at level L5-S1. The patient failed conservative treatments prior to undergoing ...
Breaking Biology Technology: