Navigation Links
10X Automation Buffer from GeneTex

Products10X Automation Buffer from GeneTex
Company GeneTex
Item 10X Automation Buffer
Price $138.00
Description The 10X Automation Buffer is a concentrated formulation of a rinsing buffer for use in autostainers employing capillary action technology, as well as for manual immunostaining. It was developed in collaboration with David Brigati M.D., Department of Pathology, University of Oklahoma, Oklahoma City, OK. This buffer is recommended for all the rinsing steps performed when doing immunocytochemical procedures. The 10X Automation Buffer is recommended for all procedures utilizing Dr. David Brigati's capillary action technique for transporting reagents to the specimens. No pH adjustment or additives are required when using this formulation. Simply dilute the concentrated 10-fold buffer with distilled or deionized water. A total of 10 liters of working rinsing solution can be prepared with 1 liter of the concentrated reagent. This buffer is also the reagent of choice for rinsing tissue sections that are subjected to immunocytochemical staining using conventional horizontal processing techniques. This concentrated buffer is recommended for the rinsing steps when performing most immunoassays including immunohistochemical staining of tissue sections using any of our HistoScan(tm), LymphoScan(tm) or BioStain(tm) Super ABC kits. Seven Step Procedure: 1.After the immunostaining, rinse slides with distilled water and drain. 2.Introduce the hematoxylin. 3.Incubate the slides for 1-2 min. at room temperature. 4.Drain off the hematoxylin and wash slides several times with distilled water. 5.Incubate the slides with 1X Automation Buffer or PBS (pH7.4) for 1 minute. 6.Wash with distilled water. 7.Mount with Crystal/Mount (Cat. #M02 or #M03). TBS based (Tris-Buffered Saline): -- Deionized or Distilled Water -- 50mM Tris Base -- 150mM Sodium Chloride (NaCl) -- 0.02% Non-ionic detergent (Triton-X 100, NP-40, Tween-20 or BRIG 35.) detergent used to reduce background and aid in breaking of surface tension for capillary action -- Preservative --The pH of 10X buffer is 7.5, when diluted 10 times, the pH of working solution will be 7.5+/- 0.1 Alternatively: -- Substitute 10mM Phosphate for Tris Base. -- Substitute 10mM Phosphate for Tris Base.
Info GeneTexGeneTex
14785 Omicron Dr. Suite101
San Antonio, TX 78245
Customer Service: (877) 436-3839
Fax Number: (210) 677-8843
Web Site: http://www.genetex.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Biomek 3000 Laboratory Automation Workstation from Beckman Coulter
2. Biomek FXP Laboratory Automation Workstation with Single Span-8 Pod from Beckman Coulter
3. Biomek NXP Span-8 Laboratory Automation Workstation (w/o Gripper) from Beckman Coulter
4. Biomek NX P Span-8 Laboratory Automation Workstation (w/ Gripper) from Beckman Coulter
5. Biomek NXP Multichannel Laboratory Automation Workstation (with integrated Gripper) from Beckman Coulter
6. Biomek FXP Laboratory Automation Workstation with Multichannel Head from Beckman Coulter
7. Automation Certified Syringe Filters from Caliper Life Sciences
8. 10X Capillary Electrophoresis Running Buffer from Sigma-Aldrich
9. Dulbeccos Phosphate Buffered Saline (DPBS) from Sigma-Aldrich
10. Dulbeccos Phosphate Buffered Saline (DPBS) from Sigma-Aldrich
11. SlideHyb Glass Array Hybridization Buffer #1 from Ambion
Contains L-glutamine...
... Anti-PRK2 MW 140kDa ... Immunogen Peptide spanning amino acids ... Purification immunoaffinity purified Quality ... RIPA lysates from HeLa cells. ...
...
Replacement Parts for Electrophoresis Apparatus...
Biology Products:
(Date:3/2/2017)... Australia , March 2, 2017 Australian ... Ltd (ASX: CYP), has signed an agreement with ... from the Monash Biomedicine Discovery Institute and Department of ... to conduct a further preclinical study to support the ... treatment of asthma.  Asthma is a ...
(Date:2/27/2017)... 27, 2017   Strategic Cyber Ventures , the ... led a $3.5 million investment in  Polarity , the ... Ventures is DC based and is led by cybersecurity ... . Ron Gula , also a longtime cybersecurity ... in this series A round of funding. This new ...
(Date:2/21/2017)... -- Der weltweite Biobanking-Sektor wird bis zum ... mit mehr als 50 Vertretern aus verschiedenen Branchen wurde aber ... diese Prognose zu realisieren. ... Zu den Schwierigkeiten für ... für die Biobank, die Implementierung Zeit sparender Technologien, ein ...
Breaking Biology News(10 mins):
(Date:3/23/2017)... ... March 23, 2017 , ... Lajollacooks4u is proud to announce it has become the ... corporate cooking challenges for companies around the world, such as Illumina, HP and Qualcomm, and ... reason for its increasing popularity is due to its new team building format, a way ...
(Date:3/23/2017)... MILFORD, Mass. , March 23, 2017 ... leading partner to global in vitro diagnostics ... launch of the industry,s first multiplexed ... inherited disease testing by next-generation sequencing ... materials were developed with input from industry ...
(Date:3/23/2017)... ... March 23, 2017 , ... Ellen ... the Connecticut Technology Council (CTC) as a 2017 Women of Innovation® finalist. Matloff ... of Innovation Awards Dinner. , The dinner recognizes women accomplished in science, technology, ...
(Date:3/23/2017)... , March 23, 2017 According to a ... and derivatives market is fragmented due to the presence of a large ... Proliant, Thermo Fisher , and Sigma-Aldrich, compete with each other ... companies, collectively, held more than 76% of this market in 2016.  ... As of now, ...
Breaking Biology Technology: