Navigation Links
10X Automation Buffer from GeneTex

Products10X Automation Buffer from GeneTex
Company GeneTex
Item 10X Automation Buffer
Price $138.00
Description The 10X Automation Buffer is a concentrated formulation of a rinsing buffer for use in autostainers employing capillary action technology, as well as for manual immunostaining. It was developed in collaboration with David Brigati M.D., Department of Pathology, University of Oklahoma, Oklahoma City, OK. This buffer is recommended for all the rinsing steps performed when doing immunocytochemical procedures. The 10X Automation Buffer is recommended for all procedures utilizing Dr. David Brigati's capillary action technique for transporting reagents to the specimens. No pH adjustment or additives are required when using this formulation. Simply dilute the concentrated 10-fold buffer with distilled or deionized water. A total of 10 liters of working rinsing solution can be prepared with 1 liter of the concentrated reagent. This buffer is also the reagent of choice for rinsing tissue sections that are subjected to immunocytochemical staining using conventional horizontal processing techniques. This concentrated buffer is recommended for the rinsing steps when performing most immunoassays including immunohistochemical staining of tissue sections using any of our HistoScan(tm), LymphoScan(tm) or BioStain(tm) Super ABC kits. Seven Step Procedure: 1.After the immunostaining, rinse slides with distilled water and drain. 2.Introduce the hematoxylin. 3.Incubate the slides for 1-2 min. at room temperature. 4.Drain off the hematoxylin and wash slides several times with distilled water. 5.Incubate the slides with 1X Automation Buffer or PBS (pH7.4) for 1 minute. 6.Wash with distilled water. 7.Mount with Crystal/Mount (Cat. #M02 or #M03). TBS based (Tris-Buffered Saline): -- Deionized or Distilled Water -- 50mM Tris Base -- 150mM Sodium Chloride (NaCl) -- 0.02% Non-ionic detergent (Triton-X 100, NP-40, Tween-20 or BRIG 35.) detergent used to reduce background and aid in breaking of surface tension for capillary action -- Preservative --The pH of 10X buffer is 7.5, when diluted 10 times, the pH of working solution will be 7.5+/- 0.1 Alternatively: -- Substitute 10mM Phosphate for Tris Base. -- Substitute 10mM Phosphate for Tris Base.
Info GeneTexGeneTex
14785 Omicron Dr. Suite101
San Antonio, TX 78245
Customer Service: (877) 436-3839
Fax Number: (210) 677-8843
Web Site: http://www.genetex.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Biomek 3000 Laboratory Automation Workstation from Beckman Coulter
2. Biomek FXP Laboratory Automation Workstation with Single Span-8 Pod from Beckman Coulter
3. Biomek NXP Span-8 Laboratory Automation Workstation (w/o Gripper) from Beckman Coulter
4. Biomek NX P Span-8 Laboratory Automation Workstation (w/ Gripper) from Beckman Coulter
5. Biomek NXP Multichannel Laboratory Automation Workstation (with integrated Gripper) from Beckman Coulter
6. Biomek FXP Laboratory Automation Workstation with Multichannel Head from Beckman Coulter
7. Automation Certified Syringe Filters from Caliper Life Sciences
8. 10X Capillary Electrophoresis Running Buffer from Sigma-Aldrich
9. Dulbeccos Phosphate Buffered Saline (DPBS) from Sigma-Aldrich
10. Dulbeccos Phosphate Buffered Saline (DPBS) from Sigma-Aldrich
11. SlideHyb Glass Array Hybridization Buffer #1 from Ambion
... White solid. PACKAGED UNDER INERT GAS. DO ... resin with applications similar to those of DTT ... , and 233155 ) but with the ... to the system. REDUCTACRYL™ Reagent can be used ...
... HVJ (hemagglutinating virus of Japan, also named ... tool for transfection of molecules by means ... for siRNA (RNAi), Protein, Oligo, cDNA, Antibodies, ... in vivo. GenomONE-Neo EX has a high ...
... White solid.. PACKAGED UNDER INERT GAS. Cyclizes ... is "driven" to completion. A protective agent for ... in many other preparations. Blocks the lethal and ... titration. Contaminants: Heavy metals: ≤1 ppm. ...
... recent sequencing of Salmonella genomes has allowed ... ORFmer PCR* primer pairs. The S. enterica ... single chromosome (4,857,432 bp; 4,440 ORFs) and ... These ORFmers from Sigma-Genosys have been designed ...
Biology Products:
(Date:5/16/2013)... FASEB MARC (Maximizing Access to Research Careers) Program ... Society for Developmental Biology (SDB) 72nd Annual Meeting ... awards are meant to promote the entry of ... the mainstream of the basic science community and ... the SDB 2013 Annual Meeting. , Awards are ...
(Date:5/16/2013)... layer lining the body,s blood vessels, is extremely ... thickness, this super-tenuous structure routinely withstands blood flow, ... a unique and highly dynamic barrier that maintains ... body,s circulatory system. , It,s also extremely adaptable. ... breached to enable immune cells to reach ...
(Date:5/16/2013)... fossils that are about 23 million years old ... the gobioid order, one of the most species-rich ... led by paleontologist Professor Bettina Reichenbacher from the ... of Earth and Environmental Sciences at Ludwig-Maximilians-Universitaet (LMU) ... analysis of fish fossils which they assign to ...
Breaking Biology News(10 mins):Endothelium, heal thyself 2Endothelium, heal thyself 3Endothelium, heal thyself 4Paleontology: The eloquence of the otoliths 2Paleontology: The eloquence of the otoliths 3
... usually a joyous event, but when a child is born too ... baby and steep medical bills for the family. To address this, ... new system to pick up very early signs that a woman ... a pregnancy is 40 weeks, while babies born before 37 weeks ...
... 21 July, 2010 (Vienna, Austria) -- Can HIV infection ... persistence in infected persons was the topic of a ... eve of AIDS 2010, the biennial international conference of ... "Towards a Cure: HIV Reservoirs and Strategies to Control ...
... a new solution to an old debate on why species hybrids ... be published online next week in the online open access journal ... "noise" caused by a surprising degree of variation in gene activity ... that when species hybridise, some of the variation in gene activity ...
Cached Biology News:Students design early labor detector to prevent premature births 2Students design early labor detector to prevent premature births 3Students design early labor detector to prevent premature births 4Top scientists tackle the issue of HIV persistence 2What causes hybrid vigor? 2
(Date:5/17/2013)... May 17, 2013 •    First of its ... for sustainability ,     New facility will help solve ... , Syngenta unveiled its new crop research ... company’s RTP Innovation Center. The first of its kind, ... simulate any agricultural climate and precisely measure plant inputs ...
(Date:5/17/2013)... Calif. , May 17, 2013 /PRNewswire-iReach/ -- ... has been reached with Switzerland ... of anti-infective human monoclonal antibody (mAb) products, and ... human mAb products for treatment of infections by ... Staphylococcus aureus , Pseudomonas aeruginosa , ...
(Date:5/17/2013)... Frederick, MD (PRWEB) May 17, 2013 ... release of ezColony version 4.1, a versatile refinement ... world-class premier software solution, improves its advanced methodical ... animal facilities with ezColony 4.1. The network-based platform ... to conduct effective research. While continuing to emphasize ...
(Date:5/16/2013)... The Stirling Ultracold Model SU780U ... Athens, OH, USA, won the Outstanding New Product Award ... for Biological and Environmental Repositories (ISBER) at their annual ... product annually, recognizes the company’s development and introduction of ... The award was presented to Global Cooling’s CEO Neill ...
Breaking Biology Technology:Syngenta Opens Unique $72 Million Advanced Crop Lab 2Syngenta Opens Unique $72 Million Advanced Crop Lab 3Aridis Pharmaceuticals Announces Acquisition of Monoclonal Antibody Products and Technologies From Kenta Biotech 2Aridis Pharmaceuticals Announces Acquisition of Monoclonal Antibody Products and Technologies From Kenta Biotech 3RURO Introduces ezColony 4.1 – The Versatile Transgenic Animal Colony Management Software 2Stirling Ultracold Ultra-Low Freezer Wins 2013 Outstanding New Product Award at International Biorepository Conference, Sydney, Australia 2