Navigation Links
10X Automation Buffer from GeneTex

Products10X Automation Buffer from GeneTex
Company GeneTex
Item 10X Automation Buffer
Price $138.00
Description The 10X Automation Buffer is a concentrated formulation of a rinsing buffer for use in autostainers employing capillary action technology, as well as for manual immunostaining. It was developed in collaboration with David Brigati M.D., Department of Pathology, University of Oklahoma, Oklahoma City, OK. This buffer is recommended for all the rinsing steps performed when doing immunocytochemical procedures. The 10X Automation Buffer is recommended for all procedures utilizing Dr. David Brigati's capillary action technique for transporting reagents to the specimens. No pH adjustment or additives are required when using this formulation. Simply dilute the concentrated 10-fold buffer with distilled or deionized water. A total of 10 liters of working rinsing solution can be prepared with 1 liter of the concentrated reagent. This buffer is also the reagent of choice for rinsing tissue sections that are subjected to immunocytochemical staining using conventional horizontal processing techniques. This concentrated buffer is recommended for the rinsing steps when performing most immunoassays including immunohistochemical staining of tissue sections using any of our HistoScan(tm), LymphoScan(tm) or BioStain(tm) Super ABC kits. Seven Step Procedure: 1.After the immunostaining, rinse slides with distilled water and drain. 2.Introduce the hematoxylin. 3.Incubate the slides for 1-2 min. at room temperature. 4.Drain off the hematoxylin and wash slides several times with distilled water. 5.Incubate the slides with 1X Automation Buffer or PBS (pH7.4) for 1 minute. 6.Wash with distilled water. 7.Mount with Crystal/Mount (Cat. #M02 or #M03). TBS based (Tris-Buffered Saline): -- Deionized or Distilled Water -- 50mM Tris Base -- 150mM Sodium Chloride (NaCl) -- 0.02% Non-ionic detergent (Triton-X 100, NP-40, Tween-20 or BRIG 35.) detergent used to reduce background and aid in breaking of surface tension for capillary action -- Preservative --The pH of 10X buffer is 7.5, when diluted 10 times, the pH of working solution will be 7.5+/- 0.1 Alternatively: -- Substitute 10mM Phosphate for Tris Base. -- Substitute 10mM Phosphate for Tris Base.
Info GeneTexGeneTex
14785 Omicron Dr. Suite101
San Antonio, TX 78245
Customer Service: (877) 436-3839
Fax Number: (210) 677-8843
Web Site: http://www.genetex.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Biomek 3000 Laboratory Automation Workstation from Beckman Coulter
2. Biomek FXP Laboratory Automation Workstation with Single Span-8 Pod from Beckman Coulter
3. Biomek NXP Span-8 Laboratory Automation Workstation (w/o Gripper) from Beckman Coulter
4. Biomek NX P Span-8 Laboratory Automation Workstation (w/ Gripper) from Beckman Coulter
5. Biomek NXP Multichannel Laboratory Automation Workstation (with integrated Gripper) from Beckman Coulter
6. Biomek FXP Laboratory Automation Workstation with Multichannel Head from Beckman Coulter
7. Automation Certified Syringe Filters from Caliper Life Sciences
8. 10X Capillary Electrophoresis Running Buffer from Sigma-Aldrich
9. Dulbeccos Phosphate Buffered Saline (DPBS) from Sigma-Aldrich
10. Dulbeccos Phosphate Buffered Saline (DPBS) from Sigma-Aldrich
11. SlideHyb Glass Array Hybridization Buffer #1 from Ambion
(Date:12/10/2014)... -- CIE San Diego has launched an innovative trust ... that enhances care coordination among social service and care ... earned a second $1 million grant from the Alliance ... in community and; will be recognized as a "Live ... 4-6p. CIE San Diego today announced the ...
(Date:12/10/2014)... , December 9, 2014 ... BCARD Platform   ... meeting scheduling solutions for business-to-business (B2B) events, today ... leader in mobile near-field communication (NFC), Bluetooth low ...      (Logo: http://photos.prnewswire.com/prnh/20140612/691055) , Jifflenow will ...
(Date:12/5/2014)... -- Tute Genomics, a leader in cloud-based solutions for genome ... led by UK-based Eurovestech. Peak Ventures and a number ... "We are at a pivotal time in ... seeks new approaches for the diagnosis, treatment and prevention ... and CEO of Tute Genomics. "One of the main ...
Breaking Biology News(10 mins):Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 2Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 3Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 4Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 2Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 3Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3
... childhood obesity epidemic in the United States continues, researchers are ... problem. A study from the Department of Nutritional Sciences, Rutgers ... Journal of Nutrition Education and Behavior reports that mothers ... overfeed them, leading to excess weight gains during the 6 ...
... States, the Netherlands and Iceland has identified three genes ... disease risk. One of the discovered genes, the UMOD ... the urine of healthy individuals. Although the Tamm-Horsfall protein ... are not well understood and its relationship to chronic ...
... of Standards and Technology (NIST) last week issued its ... field of tissue engineering for medicine. The new NIST ... that have been measured and documented by NIST for ... under development for some years, are biodegradable materials that ...
Cached Biology News:Does mom know when enough is enough? 2Study finds novel genetic risk factors for kidney disease 2NIST issues first reference material for tissue engineering 2
(Date:12/24/2014)... December 23, 2014 “Preparative & ... HPLC, Flash), Consumables (Columns, Solvents, Buffers, Valves, Guages, ... & Agriculture) - Forecasts to 2019” provides a ... opportunities, current market trends, and strategies impacting the ... estimates and forecasts of the revenue and share ...
(Date:12/24/2014)... MA (PRWEB) December 23, 2014 ... webinar series is designed to introduce cutting ... inform users and partners of platform upgrades. ... and benefits; empowering users in their quest ... powerful tools. , According to SoundConnect’s Marketing ...
(Date:12/24/2014)... Texas (PRWEB) December 23, 2014 ... methyl mercaptan such as its definition, classification, ... presents product specification, manufacturing process, and product ... technology and applications. The analysis also covers ... marketing channels, industry development trend and proposals. ...
(Date:12/24/2014)... (PRWEB) December 23, 2014 Pipette.com announces ... their Twitter followers can submit their #HolidayInTheLab pictures for ... their lab mates. , For those struggling to think ... the Pipette.com Holiday in the Lab Contest provides the ... Twitter followers to send pictures of their chemistree, ...
Breaking Biology Technology:Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 2Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 3Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 4SoundConnect Launches Leading Edge Business Communications Webinar Series 2SoundConnect Launches Leading Edge Business Communications Webinar Series 3Worldwide Methyl Mercaptan Market 2015-2020 Forecasts on Development & Trends Now Available at ReportsnReports.com 2Major Pipette Distributor Pipette.com Announces their Holiday in the Lab Contest 2