Navigation Links
NeuroCyte - Rat Neural Stem Cell Kit from Orion BioSolutions, Inc.

ProductsNeuroCyte - Rat Neural Stem Cell Kit from Orion BioSolutions, Inc.
Company Orion BioSolutions, Inc.
Item NeuroCyte - Rat Neural Stem Cell Kit
Price $585.00
Description Rat Neural Stem Cell Kit with Medium

High quality ready-to-use cryopreserved neural stem cells (NCSs) complete with optimized base medium for growth and lineage commitment. Just thaw and plate these unique cells generated from E18 fetal rat brain in the format you need for a tissue-like mixture of in vitro stem/progenitor cells, neurons, astrocytes and oligodendrocytes. Better than neurospheres and primary E18 hippocampal preparations.

Orions scientists have developed a proprietary culture system for neural stem and progenitor cells. Using purified ECM components found in basement membranes in vivo, and our tissue-engineering expertise, we have recreated a microenvironment in vitro with the key molecules found in the germinal centers of the brain during fetal development. This process supports a unique mix of nestin expressing neuroepithelial stem cells and radial glial cell-like neuroglial progenitors, which retain the capacity for multi-lineage differentiation into astrocytes, neurons, and oligodendrocytes. NeuroCyte brings these cells to you in a ready to use format, so you dont have to waste time and money waiting for your neurospheres to grow.

Using FGF-2 as the primary mitogen in our own medium formulation which contains very low amounts of fetal bovine serum (less than 0.2%), neural stem cells are expanded for 3 to 4 passages prior to cryopreservation. You simply thaw the cryovial, add to the complete medium with ECM and bFGF provided in the kit, and plate. After 3-5 days NeuroCyte neural stem cells are ready for your experiments. This kit also provides an optimized minimal medium for multi-lineage commitment, through growth factor withdrawal and retinoic acid supplementation, perfect for experiments which examine the effects of test molecules on neural stem and progenitor cell differentiation.

Unlike neurospheres, Orions adherent culture allows for the normal planar polarity found in areas like the SVZ, where undifferentiated neuroepithelial sheets provide an anchor for directed radial glial cell process extension and subsequent neuroglial progenitor and neuroblast migration. The phase contrast photomicrographs above show how NeuroCyte neural stem cells look in culture, with an epithelial cluster below large radial glial-like cells with an extensive network of long branching processes. Once cultures are switched into the commitment base medium, growth factor withdrawal and retinoic acid induced differentiation proceeds (far right). With NeuroCyte-NSC intracellular FACS phenotyping kit, you can quantify a decrease in nestin expressing cells with an increase in baseline TUJ1/b-tubulin III neuroblasts and neurons. Orion also provides a focused microarray for gene expression analysis, StemChip.

Info Orion BioSolutions, Inc.Orion BioSolutions, Inc.
1396 Poinsettia Ave
Vista, CA 92081
Customer Service: (760) 560-0022
Fax Number: (760) 560-1031
Web Site: http://www.orionbiosolutions.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Neural Stem Cell Expansion Kit - Monolayer Plus from R&D Systems
2. Neural Stem Cell Expansion Kit - Neurosphere from R&D Systems
3. Neural Stem Cell Expansion Kit - Monolayer from R&D Systems
4. Mouse/Rat Neural Stem Cell Functional Identification Kit from R&D Systems
5. Human Neural Stem Cell Functional Identification Kit from R&D Systems
6. rNeural Stem Cell (rNSC)Kit from AlphaGenix, Inc.
... Each vial contains 200 g of ... receptor sequence (C-terminal amino acids 380-402; GLTPSAWEASSLRSSRHSGLSHF. ... an antigen for production of Cayman's EP1 ... can be used in conjunction with this ...
Anti-ZFP200 Family: Zinc Finger Peptide Sequence: CGKSFNHKTNLNKHER...
Complete cell culture media with cytokines...
Consists of 4 separate 100mM solutions (ATP,GTP,CTP and UTP pH 7.5) as sodium salts. 98% Pure by HPLC...
Biology Products:
(Date:4/9/2015)... , April 9, 2015 Synaptics Inc. (NASDAQ: ... announced today that it will report financial results for ... 23, 2015, after the close of market. The company ... investors at 2:00 p.m. PT (5:00 p.m. ET), during ... To participate on the live call, analysts and investors ...
(Date:4/2/2015)... Sweden , April 2, 2015 ... for Q1 2015 attributed to somewhat higher than 125 ... in the year-end report 2014 that revenues for Q1 ... that the company reported for Q4 2014. The operating ... to be negative. The complete interim report will as ...
(Date:3/31/2015)... March 31, 2015   Guidepoint , a leading ... the Post-Surgical Pain Management TRACKER, part of the ... trends in the medical device and therapeutics markets. The ... including treatment volumes, market share, and adoption rates across ... with joint surgery. The Post-Surgical Pain Management ...
Breaking Biology News(10 mins):Synaptics to Report Third Quarter Results on April 23 2Fingerprint Cards Revises Revenues for Q1 2015 2Guidepoint Launches Post-Surgical Pain Management TRACKER 2Guidepoint Launches Post-Surgical Pain Management TRACKER 3
... PHILADELPHIA , Feb. 20, 2013   LaserLock ... in providing state-of-the-art authentication solutions to governments, pharmaceuticals, ... a provisional patent application for a Characteristic ... simultaneously identify counterfeit products and protect against identity ...
... in the Journal of the Academy of Nutrition and ... a connection between diet and acne, particularly from high glycemic ... (MNT) can play an important role in acne treatment. ... their adolescent and young adult years. Acne influences quality of ...
... brain, patterned on engineering control theory, may some day ... and migraines, according to a Penn State researcher who ... more complex brain models emerge. "The dual ... of the most important developments in mathematics of the ...
Cached Biology News:LaserLock Technologies Files for Provisional Patent Enabling Mobile Phones with Anti-Counterfeiting Technology 2Increasing evidence links high glycemic index foods and dairy products to acne 2Engineering control theory helps create dynamic brain models 2Engineering control theory helps create dynamic brain models 3Engineering control theory helps create dynamic brain models 4
(Date:4/20/2015)... Sinovac Biotech Ltd. (Nasdaq: SVA), a leading ... unaudited financial results for the fourth quarter and full ... 2014 Financial Highlights(Compared to the fourth quarter 2013) ... of 11.4% from $22.9 million in the prior year ... pandemic influenza vaccine of $0.1 million in the fourth ...
(Date:4/20/2015)... 2015 Whitehouse Laboratories is pleased and ... most recent Food and Drug Administration (FDA) inspection and ... five day, GMP compliance inspection, as based upon 21CFR ... through April 6th and was granted NAI (no action ... the strict Good Manufacturing Practice (GMP) quality system required ...
(Date:4/17/2015)... Reed Technology and Information Services ... management, preparation, and submission to the U.S. Food and ... new Lot Distribution Report (LDR) service for manufacturers of ... with the FDA’s new rule on electronic submission of ... the volume and timing of particular biologics introduced to ...
(Date:4/17/2015)... April 17, 2015  Guggenheim Securities, the investment banking ... the hiring of William Tanner as a ... Mr. Tanner will start work at Guggenheim in May ... office. "Bill brings a wealth of ... our experienced and highly acclaimed healthcare team," said ...
Breaking Biology Technology:Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 2Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 3Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 4Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 5Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 6Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 7Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 8Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 9Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 10Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 11Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 12Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 13Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 14Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 15Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 16Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 17Sinovac Reports Unaudited Fourth Quarter and Full Year 2014 Financial Results 18Whitehouse Laboratories Completes FDA Inspection with No Form 483 – No Observations 2Whitehouse Laboratories Completes FDA Inspection with No Form 483 – No Observations 3Reed Tech Announces FDA Lot Distribution Report (LDR) Service for Manufacturers of Biologic Products 2Reed Tech Announces FDA Lot Distribution Report (LDR) Service for Manufacturers of Biologic Products 3Guggenheim Securities Hires Biotech Analyst William Tanner in Expansion of Healthcare Coverage 2