Navigation Links
NeuroCyte - Rat Neural Stem Cell Kit from Orion BioSolutions, Inc.

ProductsNeuroCyte - Rat Neural Stem Cell Kit from Orion BioSolutions, Inc.
Company Orion BioSolutions, Inc.
Item NeuroCyte - Rat Neural Stem Cell Kit
Price $585.00
Description Rat Neural Stem Cell Kit with Medium

High quality ready-to-use cryopreserved neural stem cells (NCSs) complete with optimized base medium for growth and lineage commitment. Just thaw and plate these unique cells generated from E18 fetal rat brain in the format you need for a tissue-like mixture of in vitro stem/progenitor cells, neurons, astrocytes and oligodendrocytes. Better than neurospheres and primary E18 hippocampal preparations.

Orions scientists have developed a proprietary culture system for neural stem and progenitor cells. Using purified ECM components found in basement membranes in vivo, and our tissue-engineering expertise, we have recreated a microenvironment in vitro with the key molecules found in the germinal centers of the brain during fetal development. This process supports a unique mix of nestin expressing neuroepithelial stem cells and radial glial cell-like neuroglial progenitors, which retain the capacity for multi-lineage differentiation into astrocytes, neurons, and oligodendrocytes. NeuroCyte brings these cells to you in a ready to use format, so you dont have to waste time and money waiting for your neurospheres to grow.

Using FGF-2 as the primary mitogen in our own medium formulation which contains very low amounts of fetal bovine serum (less than 0.2%), neural stem cells are expanded for 3 to 4 passages prior to cryopreservation. You simply thaw the cryovial, add to the complete medium with ECM and bFGF provided in the kit, and plate. After 3-5 days NeuroCyte neural stem cells are ready for your experiments. This kit also provides an optimized minimal medium for multi-lineage commitment, through growth factor withdrawal and retinoic acid supplementation, perfect for experiments which examine the effects of test molecules on neural stem and progenitor cell differentiation.

Unlike neurospheres, Orions adherent culture allows for the normal planar polarity found in areas like the SVZ, where undifferentiated neuroepithelial sheets provide an anchor for directed radial glial cell process extension and subsequent neuroglial progenitor and neuroblast migration. The phase contrast photomicrographs above show how NeuroCyte neural stem cells look in culture, with an epithelial cluster below large radial glial-like cells with an extensive network of long branching processes. Once cultures are switched into the commitment base medium, growth factor withdrawal and retinoic acid induced differentiation proceeds (far right). With NeuroCyte-NSC intracellular FACS phenotyping kit, you can quantify a decrease in nestin expressing cells with an increase in baseline TUJ1/b-tubulin III neuroblasts and neurons. Orion also provides a focused microarray for gene expression analysis, StemChip.

Info Orion BioSolutions, Inc.Orion BioSolutions, Inc.
1396 Poinsettia Ave
Vista, CA 92081
Customer Service: (760) 560-0022
Fax Number: (760) 560-1031
Web Site: http://www.orionbiosolutions.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Neural Stem Cell Expansion Kit - Monolayer Plus from R&D Systems
2. Neural Stem Cell Expansion Kit - Neurosphere from R&D Systems
3. Neural Stem Cell Expansion Kit - Monolayer from R&D Systems
4. Mouse/Rat Neural Stem Cell Functional Identification Kit from R&D Systems
5. Human Neural Stem Cell Functional Identification Kit from R&D Systems
6. rNeural Stem Cell (rNSC)Kit from AlphaGenix, Inc.
(Date:5/16/2017)... 16, 2017   Bridge Patient Portal , ... MD EMR Systems , an electronic medical ... GE, have established a partnership to build an ... the GE Centricity™ products, including Centricity Practice Solution ... These new integrations will allow healthcare delivery ...
(Date:4/24/2017)... -- Janice Kephart , former 9/11 Commission ... LLP (IdSP) , today issues the following statement: ... 6, 2017 Executive Order: Protecting the Nation ... instilled with greater confidence, enabling the reactivation of ... are suspended by until at least July 2017). ...
(Date:4/18/2017)... , April 18, 2017  Socionext Inc., a global expert in ... media edge server, the M820, which features the company,s hybrid codec ... provided by Tera Probe, Inc., will be showcased during the upcoming ... NAB show at the Las Vegas Convention ... Click ...
Breaking Biology News(10 mins):
(Date:8/16/2017)... ... August 16, 2017 , ... Tunnell Consulting ... sessions at the ISPE Annual Meeting and Expo , to be held October ... The event’s theme is “Driving innovation to advance patient therapies.” , The ISPE Annual ...
(Date:8/16/2017)... ... August 16, 2017 , ... Today, 3Bar Biologics ... secured $2M in funding from an impressive group of investors, including Rev1 Ventures, ... Fund. With this investment, 3Bar is broadening availability of its groundbreaking offering that ...
(Date:8/15/2017)... After spending the past two years building a state-of-the-art technology which ... offers this platform to healthcare stakeholders (hospitals, foundations, biopharma companies etc.) ... collection vis a vis their members, under their own brand. Three ... offer. ... ...
(Date:8/15/2017)... ... August 15, 2017 , ... Any expert in stem cell research or ... for more than half a century. Despite their essential roles in human health ... known that molecular tags developed for this purpose also tag other, more abundant, non-stem ...
Breaking Biology Technology: