Navigation Links
NeuroCyte - Rat Neural Stem Cell Kit from Orion BioSolutions, Inc.

ProductsNeuroCyte - Rat Neural Stem Cell Kit from Orion BioSolutions, Inc.
Company Orion BioSolutions, Inc.
Item NeuroCyte - Rat Neural Stem Cell Kit
Price $585.00
Description Rat Neural Stem Cell Kit with Medium

High quality ready-to-use cryopreserved neural stem cells (NCSs) complete with optimized base medium for growth and lineage commitment. Just thaw and plate these unique cells generated from E18 fetal rat brain in the format you need for a tissue-like mixture of in vitro stem/progenitor cells, neurons, astrocytes and oligodendrocytes. Better than neurospheres and primary E18 hippocampal preparations.

Orions scientists have developed a proprietary culture system for neural stem and progenitor cells. Using purified ECM components found in basement membranes in vivo, and our tissue-engineering expertise, we have recreated a microenvironment in vitro with the key molecules found in the germinal centers of the brain during fetal development. This process supports a unique mix of nestin expressing neuroepithelial stem cells and radial glial cell-like neuroglial progenitors, which retain the capacity for multi-lineage differentiation into astrocytes, neurons, and oligodendrocytes. NeuroCyte brings these cells to you in a ready to use format, so you dont have to waste time and money waiting for your neurospheres to grow.

Using FGF-2 as the primary mitogen in our own medium formulation which contains very low amounts of fetal bovine serum (less than 0.2%), neural stem cells are expanded for 3 to 4 passages prior to cryopreservation. You simply thaw the cryovial, add to the complete medium with ECM and bFGF provided in the kit, and plate. After 3-5 days NeuroCyte neural stem cells are ready for your experiments. This kit also provides an optimized minimal medium for multi-lineage commitment, through growth factor withdrawal and retinoic acid supplementation, perfect for experiments which examine the effects of test molecules on neural stem and progenitor cell differentiation.

Unlike neurospheres, Orions adherent culture allows for the normal planar polarity found in areas like the SVZ, where undifferentiated neuroepithelial sheets provide an anchor for directed radial glial cell process extension and subsequent neuroglial progenitor and neuroblast migration. The phase contrast photomicrographs above show how NeuroCyte neural stem cells look in culture, with an epithelial cluster below large radial glial-like cells with an extensive network of long branching processes. Once cultures are switched into the commitment base medium, growth factor withdrawal and retinoic acid induced differentiation proceeds (far right). With NeuroCyte-NSC intracellular FACS phenotyping kit, you can quantify a decrease in nestin expressing cells with an increase in baseline TUJ1/b-tubulin III neuroblasts and neurons. Orion also provides a focused microarray for gene expression analysis, StemChip.

Info Orion BioSolutions, Inc.Orion BioSolutions, Inc.
1396 Poinsettia Ave
Vista, CA 92081
Customer Service: (760) 560-0022
Fax Number: (760) 560-1031
Web Site: http://www.orionbiosolutions.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Neural Stem Cell Expansion Kit - Monolayer Plus from R&D Systems
2. Neural Stem Cell Expansion Kit - Neurosphere from R&D Systems
3. Neural Stem Cell Expansion Kit - Monolayer from R&D Systems
4. Mouse/Rat Neural Stem Cell Functional Identification Kit from R&D Systems
5. Human Neural Stem Cell Functional Identification Kit from R&D Systems
6. rNeural Stem Cell (rNSC)Kit from AlphaGenix, Inc.
... Tube Disperser System from IKA Works is the ... cleaner, safer, and more efficient way to disperse ... 2 to 15 ml inside sealed tubes. Depending ... of 4 different tube types. Each tube is ...
... newest member of Wyatt Technology's family of ... of your high temperature chromatograph and enables ... from thermoplastic polymers. The HTmD is easily ... 150C, PL220C or Alliance 2000 device and ...
... Experion StdSens reagents and supplies, for ... supplies sufficient to perform standard-sensitivity RNA ... on the Experion automated electrophoresis system. ... 20 microliters RNA StdSens stain, 20 ...
...
Biology Products:
(Date:5/24/2016)... 2016 Ampronix facilitates superior patient care by providing unparalleled technology to leaders ... is the latest premium product recently added to the range of products distributed by ... ... ... LCD Medical Display- Ampronix News ...
(Date:5/20/2016)... MINNEAPOLIS , May 20, 2016  VoiceIt ... technology partnership with VoicePass. By working ... user experience.  Because VoiceIt and VoicePass take slightly ... two engines increases both security and usability. ... expressed excitement about this new partnership. ...
(Date:5/16/2016)... 2016   EyeLock LLC , a market leader ... of an IoT Center of Excellence in ... development of embedded iris biometric applications. EyeLock,s ... and security with unmatched biometric accuracy, making it the ... DNA. EyeLock,s platform uses video technology to deliver a ...
Breaking Biology News(10 mins):
(Date:5/23/2016)... ... May 23, 2016 , ... ... molecular nanotechnology, announced the winners for the 2015 Foresight Institute Feynman Prizes. ... given in two categories, one for experiment and the other for theory in ...
(Date:5/20/2016)... ... , ... The recent recall by Costco and Trader Joes of 47 million ... 12, 2016(1), demonstrates the need for faster and more cost effective bio-threat detection to ... , PathSensor’s latest solution uses a biosensor technology called CANARY®. ...
(Date:5/19/2016)... SAN DIEGO , May 19, 2016 /PRNewswire/ ... (OTCBB: RGBP), (OTC PINK: RGBP) and (OTC PINK: RGBPP) announced ... at creating the first cord blood based cancer ... a provisional patent application, Regen described a generation ... activity was potentiated by gene silencing.  The product ...
(Date:5/18/2016)... ... May 18, 2016 , ... STACS DNA Inc., the sample tracking software company, ... sexual assault kit processing to help them save time and reduce errors. , Sexual ... to be processed and victims informed of results. Due to a previous lack of ...
Breaking Biology Technology: