Navigation Links
* Quick & Easy Conditional Knock Out Kit - FRT from Gene Bridges GmbH

Products* Quick & Easy Conditional Knock Out Kit - FRT from Gene Bridges GmbH
Company Gene Bridges GmbH
Item * Quick & Easy Conditional Knock Out Kit - FRT
Description This kit is designed for the fast and simple integration of FRT-sites into large vector plasmids at any position (for the generation of transgenic mouse models). The included FRT-PGK-gb2-neo-FRT cassette is designed to allow neomycin / kanamycin selection in prokaryotic and eukaryotic cells. It combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eukaryotic promoter (PGK) for expression of neomycin resistance in mammalian cells. The included pCI-FLPe expression plasmid codes for an improved Flp recombinase which shows a four to tenfold higher recombinational activity compared to the wildtype Flp enzyme at temperatures between 37C (optimal growth temperature for E.coli) and 40C (mouse body temperature). This kit can also be used to work on bacterial artificial chromosomes (BACs). The generation of targeting constructs is achieved utilizing the high Red/ET efficiency plus the convenient removal of the Red/ET Recombineering protein expression plasmid pRedET after recombination.
The kit is available to academic researchers. Commercial organizations require a sub-license from Gene Bridges.

  • Additional Specifications: Also available as as service.
Info Gene Bridges GmbH
Commercial Centre
Im Neuenheimer Feld 584
69120 Heidekberg, Germany
Customer Service: +49 (0)6221 13708 10
Fax Number: +49 (0)6221 13708 29
Web Site:
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. * Quick & Easy Conditional Knock Out Kit - loxP from Gene Bridges GmbH
2. QuickLane Individual Sample Sequencing from Agencourt Bioscience
3. * Quick & Easy E. coli Gene Deletion Kit from Gene Bridges GmbH
4. TNT® T7 Quick Coupled Transcription/Translation System from Promega
5. TargeTron Gene Knockout System from Sigma-Aldrich
6. FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
7. loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH
8. FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH
9. loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
10. FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
11. loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
... drug discovery system miniaturizes, integrates and ... Using LabChip technology, the LabChip 3000 ... continuous flow fashion. LabChip assays are ... exceeds what is achievable in homogenous, ...
Biology Products:
(Date:4/15/2016)... CHICAGO , April 15, 2016  A ... companies make more accurate underwriting decisions in a ... offering timely, competitively priced and high-value life insurance ... health screenings. With Force Diagnostics, rapid ... and lifestyle data readings (blood pressure, weight, pulse, ...
(Date:4/14/2016)... , April 14, 2016 ... Malware Detection, today announced the appointment of Eyal ... new role. Goldwerger,s leadership appointment comes at ... heels of the deployment of its platform at several ... biometric technology, which discerns unique cognitive and physiological factors, ...
(Date:3/31/2016)... 31, 2016   ... the "Company") LegacyXChange is excited to release ... soon to be launched online site for trading 100% ... ) will also provide potential shareholders a sense of ... to an industry that is notorious for fraud. The ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... ... is pleased to announce the launch of their brand, UP4™ Probiotics, into Target ... over 35 years, is proud to add Target to its list of well-respected ...
(Date:6/23/2016)... 23, 2016 Houston Methodist Willowbrook Hospital ... Sports Association to serve as their official health ... Methodist Willowbrook will provide sponsorship support, athletic training ... association coaches, volunteers, athletes and families. ... Sports Association and to bring Houston Methodist quality ...
(Date:6/23/2016)... --  EpiBiome , a precision microbiome engineering company, today ... from Silicon Valley Bank (SVB). The financing will allow ... drug development efforts, as well as purchase additional lab ... been an incredible strategic partner to us – one ... provide," said Dr. Aeron Tynes Hammack , EpiBiome,s ...
(Date:6/23/2016)... LOUISVILLE, Ky. , June 23, 2016 /PRNewswire/ ... from two Phase 1 clinical trials of its ... double-blind, placebo-controlled, single and multiple ascending dose studies ... and pharmacodynamics (PD) of subcutaneous injection in healthy ... APL-2 subcutaneously (SC) either as a single dose ...
Breaking Biology Technology: