Navigation Links
* Quick & Easy Conditional Knock Out Kit - FRT from Gene Bridges GmbH

Products* Quick & Easy Conditional Knock Out Kit - FRT from Gene Bridges GmbH
Company Gene Bridges GmbH
Item * Quick & Easy Conditional Knock Out Kit - FRT
Price 
Description This kit is designed for the fast and simple integration of FRT-sites into large vector plasmids at any position (for the generation of transgenic mouse models). The included FRT-PGK-gb2-neo-FRT cassette is designed to allow neomycin / kanamycin selection in prokaryotic and eukaryotic cells. It combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eukaryotic promoter (PGK) for expression of neomycin resistance in mammalian cells. The included pCI-FLPe expression plasmid codes for an improved Flp recombinase which shows a four to tenfold higher recombinational activity compared to the wildtype Flp enzyme at temperatures between 37C (optimal growth temperature for E.coli) and 40C (mouse body temperature). This kit can also be used to work on bacterial artificial chromosomes (BACs). The generation of targeting constructs is achieved utilizing the high Red/ET efficiency plus the convenient removal of the Red/ET Recombineering protein expression plasmid pRedET after recombination.
The kit is available to academic researchers. Commercial organizations require a sub-license from Gene Bridges.

  • Additional Specifications: Also available as as service.
Info Gene Bridges GmbH
Commercial Centre
Im Neuenheimer Feld 584
69120 Heidekberg, Germany
Customer Service: +49 (0)6221 13708 10
Fax Number: +49 (0)6221 13708 29
Web Site: http://www.Genebridges.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. * Quick & Easy Conditional Knock Out Kit - loxP from Gene Bridges GmbH
2. QuickLane Individual Sample Sequencing from Agencourt Bioscience
3. * Quick & Easy E. coli Gene Deletion Kit from Gene Bridges GmbH
4. TNT® T7 Quick Coupled Transcription/Translation System from Promega
5. TargeTron Gene Knockout System from Sigma-Aldrich
6. FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
7. loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH
8. FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH
9. loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
10. FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
11. loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
DU 530 Life Science UV/Visible Spectrophotometers from Beckman Coulter
The model 55D Rocking Shaker provides the same Reliable quality as the 55S, but double the value. The 55D gives you an additional platform with a height of 3.25 in.
...t will help protect your PCR runs against contamin... to use, reducing the possibility that contaminati...uce the chance of airborne contamination., The non...iew, an acid-resistant black formica work surface,...
Have you bought a Perkin Elmer UltraVIEW system and want to enjoy the sensitivity of using the Andor iXon and iQ software? No problem. Our experienced team can give you that upgrade. You keep your e
Biology Products:
(Date:5/16/2008)... and denials of shipments involving regulated radi...e of growing concern to safety and industry expert...they agreed on an action plan for the Mediterranea...s, research centres and organizations that rely on..., The shipment issues have surfaced over the pas...
(Date:5/16/2008)...al concerns in maintaining germ-free homes for the...and the rapid adoption of anti-bacterial soaps and...ose antiseptic soaps now have come under scrutiny ...human health concerns. , Two closely related a...the center of the debacle. Whereas triclosan (TCS)...
(Date:5/15/2008)...E Evolution is supposed to inch forward over eons...fish called the threespine stickleback, the proces... to a study led by researchers at Fred Hutchinson ...of print in the May 20 issue of Current Biology (C...les of reverse evolution in nature, said senior au...
(Date:5/15/2008)...E STATION A large quantity of nitrogen compounds ...burning of fossil fuels and the use of nitrogen fe...moval of some carbon dioxide from the atmosphere, ...y Texas A&M University Distinguished Professor of ... , The team of 30 experts from institutions arou...
Breaking Biology News(10 mins):Experts tackle shipment issues for beneficial radiation sources 2Experts tackle shipment issues for beneficial radiation sources 3New study links fate of personal care products to environmental pollution and human health concerns 2New study links fate of personal care products to environmental pollution and human health concerns 3Researchers document rapid, dramatic 'reverse evolution' in the threespine stickleback fish 2Researchers document rapid, dramatic 'reverse evolution' in the threespine stickleback fish 3Atmosphere threatened by pollutants entering ocean, prof says 2Atmosphere threatened by pollutants entering ocean, prof says 3LogistiCare a Subsidiary of Providence Service Corp Opens Phoenix Call Center to Support Expanding Commercial Client Base 9774 1LogistiCare a Subsidiary of Providence Service Corp Opens Phoenix Call Center to Support Expanding Commercial Client Base 9774 2LogistiCare a Subsidiary of Providence Service Corp Opens Phoenix Call Center to Support Expanding Commercial Client Base 9774 3Schering Plough CEO to Buy 242 Million in Common Shares 9770 1Schering Plough CEO to Buy 242 Million in Common Shares 9770 2Schering Plough CEO to Buy 242 Million in Common Shares 9770 3Sigma Aldrich Corporations 4th Quarter 2007 Earnings Conference Call 2659 1Sigma Aldrich Corporations 4th Quarter 2007 Earnings Conference Call 2659 2Kiwa Bio Tech Products Group Holds Meetings to Build Distribution Network 2655 1Kiwa Bio Tech Products Group Holds Meetings to Build Distribution Network 2655 2Kiwa Bio Tech Products Group Holds Meetings to Build Distribution Network 2655 3
...A few years ago the study of the effects of atmosp...nd the scientific sphere and the term "acid rain" ... happens because, due to the burning of fossil fue...he atmosphere is greater than that derived from na...of water vapour and under the oxido-reduction cond...
...First step toward developing enzymes to treat dise...warfare threatsA team of biomedical researchers fr... at Austin has determined the first 3-dimensional ...enting certain bacterial infections in plants, ani... the Proceedings of the National Academy of Scienc...
...Max Planck researchers uncover how a nanoscale ,co... magnetic field. , The entire bacterium is oriente... Until now, it was not clear how the cells organis...physical tendency to collapse by magnetic attracti... processes, researchers from the Max Planck Instit...
...Cyberkinetics Neurotechnology Systems, Inc., Case ...enter today announced that they have been awarded...nal Center for Medical Rehabilitation Research (NC...ld Health and Human Development (NICHD). According... $2.3 million of the total award over the five-yea...
Other Biology News:'Acid rain' and forest mass: Another perspective 2Scientists determine structure of enzyme that disrupts bacterial virulence 2Bacteria which sense the Earth's magnetic field 2Bacteria which sense the Earth's magnetic field 3Bacteria which sense the Earth's magnetic field 4Cyberkinetics, Case to develop system to help restore extremity function 2Cyberkinetics, Case to develop system to help restore extremity function 3Cyberkinetics, Case to develop system to help restore extremity function 4Cyberkinetics, Case to develop system to help restore extremity function 5Cyberkinetics, Case to develop system to help restore extremity function 6
(Date:5/16/2008)... RALEIGH, N.C., May 16 /- Campbel... specializing in the pharmaceutical and biotech,in...kshop at the Society of,Pharmaceutical and Biotech...19-22, 2008 at the Orlando World Center Marriott i... am, George Schmidt, Vice President in the,Sales P...
(Date:5/16/2008)... MOUNTAIN VIEW, Calif., May 16 /PRNewswire-Fir...P ) today announced that the Company will,present ...care Conference,on Wednesday, May 21, 2008, at 9:0...e presentation will be available on the Investor,R...t, http://www.mappharma.com . A replay will also b...
(Date:5/15/2008)... New Device Designed to Provide Fast, Safe and...Catheterization Procedures, ABBOTT PARK, Ill., Ma...ced the launch of the StarClose(R) SE Vascular Clo...ce engineered to enable fast, safe and,secure clos...catheterization procedure. StarClose SE is availab...
(Date:5/15/2008)... Toronto Stock Exchange Symbo...BioMS Medical Corp. (TSX:,MS), a leading developer...y,announced financial and operational results for ...partnership with Eli Lilly and Company, is,develop...c peptide drug for,the treatment of MS undergoing ...
Breaking Biology Technology:Campbell Alliance Sales Practice to Present Workshop at SPBT Annual Conference 2Campbell Alliance Sales Practice to Present Workshop at SPBT Annual Conference 3Abbott Launches Next-Generation StarClose(R) SE Vascular Closure System 2Abbott Launches Next-Generation StarClose(R) SE Vascular Closure System 3BioMS Medical announces first quarter 2008 results 2BioMS Medical announces first quarter 2008 results 3BioMS Medical announces first quarter 2008 results 4BioMS Medical announces first quarter 2008 results 5
...c surgery team led by Dr. Alfred Tector has perfor... artificial heart at Aurora St. Luke,s Medical Ce...e first to introduce this new technology to our pa...transplant program, in a release. , ,The Madison-a...t failure, is reportedly in stable condition. The ...
...m Doyle selected two local residents as nominees ...ty - both of whom expressed eagerness about their ... travel. , ,Mark Hanna, a city alderman, and Judy ...gram, will join several already-named nominees for... development of an aerospace industry in the state...
...hcare will have the exclusive global distribution... the terms of a 15-year agreement with its develop...l, GE will supply the stem cell harvesting product...lood Registry , one of the world,s largest chains ...tem extracts stem cells from the umbilical cords o...
...rk owners and search engines took another turn las...sued its ruling in the case 800- JR Cigar, Inc ., ...scount cigar seller. GoTo.com is now part of Yaho... Cigar," J R Cigar," and "800 JR Cigar" to various...r article "Keyword Advertisers Win Some, Lose Some...
Other Biology Technology:Wisconsin spaceport committee moves closer to formation 2Are consumers confused by search engine ads? 2Are consumers confused by search engine ads? 3Are consumers confused by search engine ads? 4