Navigation Links
pHLIP, a novel technology to locate and treat tumors

Research teams at Yale University and the University of Rhode Island have demonstrated a new way to target and potentially treat tumors using a short piece of protein that acts like a nanosyringe to deliver “tags?or therapy to cells, according to a report in the Proceedings of the National Academy of Sciences.

The researchers show that, the protein fragment, called “pHLIP?(pH (Low) Insertion Peptide) can be injected into the abdomen of a mouse, find its way into the blood and then specifically accumulate in tumors. Within 20 hours after injection of labeled pHLIP, the molecules had passed through the bloodstream and accumulated in mouse breast tumors grown to different “stages?on the leg of a mouse.

The researchers demonstrated that by attaching fluorescent probes to a pHLIP peptide, tumors could be detected. They expect that by attaching and delivering active agents with pHLIP, that tumors may be able to be treated. Targeting is based on the fact that most tumors, even very small ones, are acidic as a result of the way they grow.

“Since the mechanism is general, and since even very small tumors can be targeted, there is an exciting array of possible applications for pHLIP,?said Donald Engelman, Eugene Higgins Professor of Molecular Biophysics & Biochemistry at Yale and a co-author of the paper.

“Andreev and Reshetnyak [co-authors of the paper] have taken a recent discovery from our lab and we are pushing hard as a team to test possible applications,?said Engelman. “We are very excited by the possibilities for both imaging and treating tumors.?

The pHLIP molecule has three states: soluble in water, bound to the surface of a membrane, and inserted across the membrane as an alpha-helix. Under normal tissue conditions of neutral pH, the water-soluble form is favored. At acidic pH, the transmembrane alp ha-helix predominates.

An earlier paper from the same groups shows that at low pH, pHLIP can move cell-impermeable molecules across a cell membrane, where they are released in the cytoplasm. “pHLIP acts as a molecular nanosyringe, inserting itself into the cell membrane and injecting compounds into cell,?said co-author Yana Reshetnyak, of the University of Rhode Island. “The transported molecules can be therapeutic or toxic to the cell, depending on the intended outcome—for treating cancer, the idea is to cause cell death.?

In addition to targeting tumors, other disease states that produce inflammation and cause tissue to be acidic are a target for pHLIP. “Acidosis is a physiological marker of many diseases ?and pHLIP feels acidity,?said Reshetnyak. “Therefore, pHLIP could also be used for monitoring of disease development and therapeutic outcomes. It might play very important role in the study of arthritis, ischemia and stroke.?

Lead author Oleg Andreev said, “We believe that universal medical tests to reveal many health problems at earlier stages may be developed based on pHLIP technology?

“Our discovery is an example of the reason that the NIH and DOD support basic science—we were working on the principles of membrane protein folding, and made a discovery with important medical implications that wouldn’t have happened without the ideas and approaches used in that work,?Engelman said.

Among the applications the team is actively pursuing are PET imaging of tumors, treatment of breast cancer, and alternative designs using the principles they have already established.

Source:Yale University

Related biology news :

1. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
2. Insight into natural cholesterol control suggests novel cholesterol-lowering therapy
3. GeneNotes - A novel information management software for biologists
4. Endocannabinoids ?the brains cannabis ?demonstrate novel modes of action to stress
5. A novel virus for croup
6. Scientists show that tick-borne flaviviruses use a novel mechanism to evade host defenses
7. A novel method to measure circadian cycles
8. UCSD discovery may provide novel method to generate medically useful proteins
9. Updated data on novel HPV vaccine confirms efficacy in large population
10. Neurons generated in the adult brain learn to respond to novel stimuli
11. Federal grant funds research on novel HIV therapy

Post Your Comments:

(Date:5/6/2017)... May 5, 2017 RAM Group ... a new breakthrough in biometric authentication based on ... mechanical properties to perform biometric authentication. These new sensors ... material created by Ram Group and its partners. This ... transportation, supply chains and security. Ram Group is ...
(Date:4/13/2017)... , April 13, 2017 UBM,s Advanced ... will feature emerging and evolving technology through ... Innovation Summits will run alongside the expo portion of ... sessions, panels and demonstrations focused on trending topics within ... advanced design and manufacturing event will take place June ...
(Date:4/11/2017)... NXT-ID, Inc. (NASDAQ:   NXTD ) ... appointment of independent Directors Mr. Robin D. Richards ... Directors, furthering the company,s corporate governance and expertise. ... Gino Pereira , Chief Executive ... their guidance and benefiting from their considerable expertise as we ...
Breaking Biology News(10 mins):
(Date:10/10/2017)... ... 2017 , ... For the second time in three years, ... Award. Representatives of the FirstHand program travelled to Washington, D.C. Tuesday, October 10th, ... mission is to change the trajectory of STEM education in America by dramatically ...
(Date:10/10/2017)... ... October 10, 2017 , ... The Pittcon Program ... honoring scientists who have made outstanding contributions to analytical chemistry and ... 2018, the world’s leading conference and exposition for laboratory science, which will be ...
(Date:10/9/2017)... ... October 09, 2017 , ... ... journal, Epilepsia, Brain Sentinel’s SPEAC® System which uses the surface electromyography (sEMG) ... tonic-clonic seizures (GTCS) using surface electromyography (sEMG). The prospective multicenter phase III ...
(Date:10/6/2017)... ... October 06, 2017 , ... On Tuesday, ... webinar on INSIGhT, the first-ever adaptive clinical trial for glioblastoma (GBM). The featured ... event is free and open to the public, but registration is required. , ...
Breaking Biology Technology: