Navigation Links
Zooming in on the protein-conducting channel

Researchers have gained the most detailed view yet of the heart of the translocon, a channel through which newly constructed proteins are inserted into the cell membrane. The process of transporting proteins across or into membranes is a critical function that occurs in every cell.

Howard Hughes Medical Institute investigator Joachim Frank at the Wadsworth Center and his colleagues reported their detailed study of the translocon's core, called the protein-conducting channel (PCC), in an article published in the November 17, 2005, issue of the journal Nature. Co-lead authors on the paper were Kakoli Mitra in Frank's laboratory and Christiane Schaffitzel of the Eidgenössische Technische Hochschule Hönggerberg in Switzerland, who is in the laboratory of the other senior author, Nenad Ban. Other co-authors were from the Scripps Research Institute and the State University of New York at Albany.

The researchers studied the PCC, which grabs newly made protein as it is extruded from the ribosome's protein synthesis machinery. The PCC then opens either a pore that is perpendicular or lateral to the cell membrane to feed the new protein either across or into the membrane.

For the studies, the Swiss researchers created a complex comprising the PCC from E. coli attached to a ribosome that contained a newly forming protein segment. The ribosome is the massive protein-RNA complex that constitutes the cell's protein-making machinery.

Mitra explored the structure of this PCC-ribosome complex using three-dimensional cryogenic electron microscopy (cryo-EM), as well as computational methods. Three-dimensional cryo-EM is one of the few techniques capable of visualizing large, dynamic molecules.

In preparing for cryo-EM, researchers immersed the PCC-containing complex in water and then abruptly froze it in supercold liquid ethane. The rapid freezing imprisoned the complex in vitreous ice, a glassy non-crystalline form of ice, thus preserving its na
'"/>

Source:HHMI


Page: 1 2 3

Related biology news :

1. UBC researchers find stroke death channel
2. Scripps Research study reveals new activation mechanism for pain sensing channel
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Zooming the protein conducting channel

(Date:7/7/2015)... July 7, 2015 NXT-ID, Inc. (NASDAQ: NXTD ) ... growing mobile commerce market announces a revised version of one of ... commercial will air on CNBC in New York ... and San Francisco metro areas.    ... on Wocket,s ability to replace all the cards in your wallet ...
(Date:6/30/2015)... To bolster its efforts and commitment to fighting cyber fraud, ... of two new team members. Dimitri ... Raviv will act as head of strategic alliances. These ... the most secure solutions for the enterprise market. ... a provider of security and management products, which was acquired ...
(Date:6/25/2015)... , June 25, 2015 ... Market by Type (Swipe & Area), Technology, Material (Optical ... Healthcare, Commercial Security & Others) & Geography - ... marketsandmarkets, the said market is expected to reach ... 17.1%. Browse 76 market Tables ...
Breaking Biology News(10 mins):Wocket Smart Wallet Commercials Airing on CNBC to Showcase This Summer's Must- Have Tech Product 2Wocket Smart Wallet Commercials Airing on CNBC to Showcase This Summer's Must- Have Tech Product 3HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 2HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 3Fingerprint Sensors Market Worth $14,500.07 Million by 2020 2Fingerprint Sensors Market Worth $14,500.07 Million by 2020 3Fingerprint Sensors Market Worth $14,500.07 Million by 2020 4
... people breathe while walking in the park, working in the ... spike their blood pressure, a new study suggests. Cardiovascular ... first to report a direct link between air pollution and ... results from these animal studies hold up, this could be ...
... time, next-generation DNA sequencing technologies have been turned on ... each year. The results will help to improve diagnosis, ... new strategies for vaccination. The study sets a ... a disease-causing bacterium: it is the first study of ...
... expectancy for patients with human immunodeficiency virus (HIV) has ... 1990s thanks to advancements in antiretroviral therapy, according to ... and Simon Fraser University in Vancouver, British Columbia. ... drop in AIDS deaths among 43,355 HIV-positive study participants ...
Cached Biology News:OSU study shows exposure to bad air raises blood pressure 2OSU study shows exposure to bad air raises blood pressure 3Defining DNA differences to track and tackle typhoid 2Defining DNA differences to track and tackle typhoid 3Anti-HIV therapy boosts life expectancy more than 13 years 2
(Date:7/27/2015)... 2015 IDEXX Laboratories, Inc. (NASDAQ: IDXX ... submitted over 100,000 specimens to IDEXX Reference Laboratories for ... 10 days since the breakthrough kidney function test launched ... leading cause of suffering and death in cats and ... with generally accepted kidney disease prevalence data showing that ...
(Date:7/24/2015)... 24, 2015 Ryan & Maniskas, LLP ... States District Court for the Northern District of ... that purchased the common stock of Avalanche Biotechnologies, Inc. ("Avalanche" ... 2014 and June 15, 2015, inclusive (the "Class Period"). ... move the Court for appointment as a lead plaintiff of ...
(Date:7/24/2015)... -- Orexigen Therapeutics, Inc. (Nasdaq: OREX ) will ... 2015 on Thursday, August 6 before the market opens. ... and discuss the financial results in a live webcast ... a.m. Pacific Time). The live call may ... or (847) 413-3238 (international), participant code 40327229. The webcast ...
(Date:7/24/2015)... , ... July 24, 2015 ... ... class-leading frozen sample management solution, indispensable to any modern scientific or pharmaceutical ... has a user-friendly interface. Users know precisely where a sample is located ...
Breaking Biology Technology:Over 100,000 Pets Tested with New IDEXX SDMA Kidney Function Test Since Product Launch on July 13 2Over 100,000 Pets Tested with New IDEXX SDMA Kidney Function Test Since Product Launch on July 13 3Over 100,000 Pets Tested with New IDEXX SDMA Kidney Function Test Since Product Launch on July 13 4Over 100,000 Pets Tested with New IDEXX SDMA Kidney Function Test Since Product Launch on July 13 5Ryan & Maniskas, LLP Announces Class Action Lawsuit Against Avalanche Biotechnologies, Inc. 2Ryan & Maniskas, LLP Announces Class Action Lawsuit Against Avalanche Biotechnologies, Inc. 3RURO, Inc. Partners with Integrated Systems Engineering to Provide Comprehensive LIMS Software to Biobanks 2RURO, Inc. Partners with Integrated Systems Engineering to Provide Comprehensive LIMS Software to Biobanks 3RURO, Inc. Partners with Integrated Systems Engineering to Provide Comprehensive LIMS Software to Biobanks 4
... 2011 Oligomerix, Inc. presented results at the ... demonstrated that tau protein forms neurotoxic oligomers with ... results in tau,s self-fragmentation and in the degradation ... neurotoxic mode-of-action. Furthermore, certain tau oligomer species that ...
... RESTON, Virginia and BRNO, Czech Republic, November 16, ... Institute (MMCI) provides complete diagnostics, treatment, and ... and international patients. MMCI is the only health ... a member of the OECI (Organisation of European ...
... Livestream Info ... http://thehill.com/video/in-the-news/193083-livestreaming-genetic-testing-briefing . , Twitter: ... redefining cancer treatment, and the pace of discovery promises new ... That will be among the topics of discussion today at ...
Cached Biology Technology:A New Mechanism for Tau Protein Pathology in Alzheimer's Disease was Presented by Oligomerix, Inc. at the Society for Neuroscience Annual Meeting 2Masaryk Memorial Cancer Institute Reduces Losses and Administrative Overhead With Ekahau RTLS 2Masaryk Memorial Cancer Institute Reduces Losses and Administrative Overhead With Ekahau RTLS 3Members of Congress, Cancer Experts Address Impact of Genetic Testing on Cancer 2
BD BioCoat Collagen IV 150 mm Culture Dishes...
Gridded Cell Culture Petri Dish (20 mm molded in base), tissue-culture treated...
MAb to Desmoplakin I & II Desmoplakin I & II...
... PI is used in conjuction with ... in the earlier stages of apoptosis (Annexin ... are in later stages of apoptosis or ... PI is detected in the orange range ...
Biology Products: