Navigation Links
Yale scientists identify structure for RNA quality control

A report by Yale scientists in the journal Cell sheds new light on how the protein Ro, a major autoantigen in patients with autoimmune disease, recognizes misfolded RNAs, creating a RNA quality control system for cells.

The quality control process in the cell has been well-studied for the DNA and messenger RNA (mRNA) components for making proteins. However, little was known about what cells do with abnormal or misfolded RNAs that are not translated into protein -- such as ribosomal RNAs (rRNAs), transfer RNAs (tRNAs), and small nuclear and cytoplasmic regulatory RNAs. This work describes a molecular mechanism of RNA quality control.

In the autoimmune disorders systemic lupus erythematosus and Sjogren's syndrome, Ro is known to be an autoantigen, and autoimmune disease develops in mice that lack Ro protein. This study demonstrated a connection between binding of normal RNA and "disposal" of abnormal and misfolded RNAs by Ro protein.

Collaboration between the laboratories of Assistant Professor Karin M. Reinisch and Associate Professor Sandra L. Wolin in the department of Cell Biology used both crystallography and biochemistry to visualize how Ro interacts with these RNAs.

"The crystal structures of Ro revealed two distinct RNA binding sites, one of which recognizes misfolded small RNAs. Unlike most proteins, Ro has a hole through the middle - and the hole is used in distinguishing these RNAs," said Reinisch.

Mothers with anti-Ro antibodies often have babies with heart signal conduction defects; some scientists believe that the antibodies may cause the defects. "Although Ro is a major human autoantigen, how the molecule is recognized by patient autoantibodies was not fully determined. So, understanding these features of the Ro protein may allow the design of drugs to block the interaction between the antibodies and Ro," said Wolin.

The research team consisted of members from the Reinisch and Wolin lab groups-- Adam J. Ste in, Gabriele Fuchs, Chumei Fu. Wolin is also an investigator of the Howard Hughes Medical Institute. The work was funded by grants from the G. Harold and Leila Y. Mathers Foundation, the Pew Charitable Trust, and the National Institutes of Health. A report by Yale scientists in the journal Cell sheds new light on how the protein Ro, a major autoantigen in patients with autoimmune disease, recognizes misfolded RNAs, creating a RNA quality control system for cells.

The quality control process in the cell has been well-studied for the DNA and messenger RNA (mRNA) components for making proteins. However, little was known about what cells do with abnormal or misfolded RNAs that are not translated into protein -- such as ribosomal RNAs (rRNAs), transfer RNAs (tRNAs), and small nuclear and cytoplasmic regulatory RNAs. This work describes a molecular mechanism of RNA quality control.

In the autoimmune disorders systemic lupus erythematosus and Sjogren's syndrome, Ro is known to be an autoantigen, and autoimmune disease develops in mice that lack Ro protein. This study demonstrated a connection between binding of normal RNA and "disposal" of abnormal and misfolded RNAs by Ro protein.

Collaboration between the laboratories of Assistant Professor Karin M. Reinisch and Associate Professor Sandra L. Wolin in the department of Cell Biology used both crystallography and biochemistry to visualize how Ro interacts with these RNAs.

"The crystal structures of Ro revealed two distinct RNA binding sites, one of which recognizes misfolded small RNAs. Unlike most proteins, Ro has a hole through the middle - and the hole is used in distinguishing these RNAs," said Reinisch.

Mothers with anti-Ro antibodies often have babies with heart signal conduction defects; some scientists believe that the antibodies may cause the defects. "Although Ro is a major human autoantigen, how the molecule is recognized by patient autoantibodies was not fully determined. So, understanding these features of the Ro protein may allow the design of drugs to block the interaction between the antibodies and Ro," said Wolin.

The research team consisted of members from the Reinisch and Wolin lab groups-- Adam J. Stein, Gabriele Fuchs, Chumei Fu. Wolin is also an investigator of the Howard Hughes Medical Institute. The work was funded by grants from the G. Harold and Leila Y. Mathers Foundation, the Pew Charitable Trust, and the National Institutes of Health.


'"/>

Source:Yale University


Related biology news :

1. Wisconsin scientists grow critical nerve cells
2. UCSB scientists probe sea floor venting to gain understanding of early life on Earth
3. UAB scientists discover the origin of a mysterious physical force
4. Fox Chase Cancer Center scientists identify immune-system mutation
5. Weizmann Institute scientists develop a new approach for directing treatment to metastasized prostate cancer in the bones.
6. U-M scientists find genes that control growth of common skin cancer
7. UCLA scientists transform HIV into cancer-seeking missile
8. RNA project to create language for scientists worldwide
9. Carnegie Mellon scientists develop tool that uses MRI to visualize gene expression in living animals
10. To control germs, scientists deploy tiny agents provocateurs
11. Leprosy microbes lead scientists to immune discovery
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/23/2017)... -- Hunova, the first robotic gym for the rehabilitation and functional motor sense ... Genoa, Italy . The first 30 robots will be available ... USA . The technology was developed and patented at the IIT ... Movendo Technology thanks to a 10 million euro investment from entrepreneur Sergio ... ...
(Date:5/16/2017)... May 16, 2017  Veratad Technologies, LLC ( www.veratad.com ... age and identity verification solutions, announced today they will ... 2017, May 15 thru May 17, 2017, in ... International Trade Center. Identity impacts the ... in today,s quickly evolving digital world, defining identity is ...
(Date:5/16/2017)... --  Bridge Patient Portal , an enterprise patient ... Systems , an electronic medical record solutions developer ... a partnership to build an interface between the ... products, including Centricity Practice Solution (CPS), Centricity Business ... integrations will allow healthcare delivery networks using GE ...
Breaking Biology News(10 mins):
(Date:5/26/2017)... ... 2017 , ... Activate Healthcare, a leading provider of on-site ... growing private companies, has selected Twine for its Employee Health Activation Platform. Activate ... model that empowers deep collaboration and behavior change. In randomized controlled trials, Twine ...
(Date:5/26/2017)... ... May 25, 2017 , ... Throughout this ... studies, describing how process development and economic goals were achieved in both industry ... a hollow-fiber bioreactor system, along with techniques for scaling production of mesenchymal stem ...
(Date:5/26/2017)... ... 25, 2017 , ... Studying biological events while they happen ... imaging using fluorescence microscopy is the perfect approach to explore these complex questions. ... be discussed, from small animal models and tissues to individual organelle and protein ...
(Date:5/26/2017)... ... May 25, 2017 , ... LabRoots , the leading ... scientists from around the world, is announcing a new textbook scholarship, the second scholarship ... and graduate students, 17 years or older, pursuing a degree in one of the ...
Breaking Biology Technology: