Navigation Links
Virologists make major step towards understanding the process of HIV infection

A working group of virologists headed by Professor Hans-Georg Kräusslich at Heidelberg University Hospitals, jointly with Professor Hanswalter Zentgraf, Division of Applied Tumor Virology of the Deutsches Krebsforschungszentrum (German Cancer Research Center, DKFZ), have been the first to label Human Immunodeficiency Viruses (HIV) for visual investigations without inhibiting the functional characteristics of the virus.

The labeling permits scientists to observe the behavior of the virus when it enters a host cell, during replication and when it leaves a cell. This is a major step towards understanding the process of HIV infection.

Modern imaging technologies facilitate real-time observation of virus-cell interactions. Many of these investigation methods require labeling of the object of interest, such as by introducing the genetic code of green fluorescent protein (GFP) into its genetic information. There the marker protein will be produced by the cellular machinery and appended to the desired site.

To observe the interaction of HIV with the host cell, there had also been attempts to label the virus with GFP. However, the genetic modifications impaired the formation of virus particles or their infectiousness, thus limiting the value of results obtained. A team of Heidelberg researchers of the university hospitals?Virology Section and the DKFZ have now found an area within the structure molecule of the viral capsid that tolerates the substantial extension by GFP. Although the insertion of the GFP molecule enlarges the HIV structure protein by about one half, infectious viruses continue to be generated. Using electron microscopy, Zentgraf’s team was able to show that appearance and shape of the virus particles thus created cannot be distinguished from normal HIV. By simultaneous production of GFP-extended and normal structure protein, PD Dr. Barbara Müller was able to produce, under a fluorescence microscope, clearly visible HIVs with several th
'"/>

Source:Deutsches Krebsforschungszentrum (German Cancer Research Center, DKFZ)


Page: 1 2

Related biology news :

1. Jefferson Virologists Coax HIV Out of Hiding
2. Columbia research lifts major hurdle to gene therapy for cancer
3. Researchers uncover sequence of major rice pathogen
4. Emergence of cancer as major cause of childhood death in developing countries is not being adequately addressed
5. Biochemists report discovery of structure of major piece of telomerase; implications for cancer
6. Biota makes major antiviral discovery
7. New gene scanning technology marks a major advance in disease research
8. LIAI scientists make major finding on potential smallpox treatment
9. MicroRNAs have shaped the evolution of the majority of mammalian genes
10. MIT: Oceans are a major gene swap-meet for plankton
11. U-M scientists identify major psoriasis susceptibility gene
Post Your Comments:
(Date:3/17/2015)... , March 17, 2015 Emotient, ... analytics software, today announced general availability of ... service for the analysis of facial expressions. The ... advertising, media content, products and services. It delivers ... engagement and sentiment - as derived from facial ...
(Date:3/16/2015)... Between 16 and 20 ... innovations in biometric identification at the CeBIT exhibition ...      (Photo: http://photos.prnewswire.com/prnh/20150316/735333 ) , ... at the forefront: the company,s all new BiometricGate ... Germany . BiometricGate is the first ...
(Date:3/12/2015)... -- The mascots of the Atlantic Coast Conference spent ... Forest Baptist Medical Center. They stopped by as part ... Basketball Tournament. Young patients enjoyed bonding with their favorite ... Brenner Children,s Hospital is the pediatric arm ... hospital in northwest North Carolina ...
Breaking Biology News(10 mins):New Emotient Web Service Measures Audience Response Using On-Demand Facial Expression Analysis 2New Emotient Web Service Measures Audience Response Using On-Demand Facial Expression Analysis 3New Emotient Web Service Measures Audience Response Using On-Demand Facial Expression Analysis 4DERMALOG Presents its Latest Biometric Innovations at the Hannover CeBIT Exhibition, 16 - 20 March 2015 2DERMALOG Presents its Latest Biometric Innovations at the Hannover CeBIT Exhibition, 16 - 20 March 2015 3
... (PINK SHEETS: DLSC) announces the appointment of Randall ... McCormick replaces outgoing President Mr. Berge Heede who resigned for personal ... "resigning for personal reasons and to allow Mr. McCormick to take ... the current Treasurer, will maintain that responsibility as well. , ...
... Legal loopholes and insufficient law enforcement mean that ... market in Asia, says a new report from ... report also raises concerns that legal provisions governing ... illegal trade in wild-caught, highly-endangered Asian elephants from ...
... It,s common knowledge that a protective navy of ... least temporarily disturb the normal balance. But it,s unclear ... full array of "good bacteria" return promptly or remain ... mice, University of Michigan scientists have shown for the ...
Cached Biology News:delSECUR CORPORATION Announces Appointment of the Company's President & CEO 2Elephant-size loopholes sustain Thai ivory trade 2Antibiotics take toll on beneficial microbes in gut 2Antibiotics take toll on beneficial microbes in gut 3
(Date:3/26/2015)... WriteResult, a premier provider of ePRO technologies and ... of Public Health and New Haven Farms to capture ... project. The goal of the study was to evaluate ... access to fresh produce and nutrition education on food ... blood pressure, diabetes and weight gain. Patients completed an ...
(Date:3/25/2015)... 2015 The surging prevalence of ... fuelled social media growth, with over 50% of ... social networking. Insurance companies are making use of ... and promote a diversity of their services and ... exploiting social networking services as part of sales ...
(Date:3/25/2015)... London, UK (PRWEB) March 25, 2015 ... of natural ingredients in all forms of food nutrients, ... led to a substantial rise in demand for natural ... astaxanthin have started to ramp up their capacities to ... for both natural and synthetic source astaxanthin (used in ...
(Date:3/25/2015)... ST LOUIS and the HAGUE, ... publication of two-year results of LES Stimulation therapy for ... of the journal Surgery [ Surgery ... and efficacy of its minimally-invasive therapy. ... an average duration of 11 years and taking prescribed ...
Breaking Biology Technology:Yale and New Haven Farms Improving Patient Health with WriteResult's myPROpad™ 2Yale and New Haven Farms Improving Patient Health with WriteResult's myPROpad™ 3Social Media Role in Global Insurance Industry Examined by Timetric in New Market Research Report Published at MarketPublishers.com 2Social Media Role in Global Insurance Industry Examined by Timetric in New Market Research Report Published at MarketPublishers.com 3Global Astaxanthin Market to Reach Value of USD 1.1 billion by 2020, Expects Industry Experts in New Report Available at MarketPublishers.com 2EndoStim LES Stimulation therapy demonstrates long term safety and efficacy results in treating reflux patients 2EndoStim LES Stimulation therapy demonstrates long term safety and efficacy results in treating reflux patients 3
... With dimensions measuring billionths of a meter, nanoparticles are way ... is becoming possible for today,s scientists not only to see ... are arranged in three dimensions using a technique called nanocrystallography. ... as x-ray synchrotrons, are only available at a handful of ...
... 4, 2012  Andrew Technologies, LLC launched the HydraSolve™ ... Aesthetic Plastic Surgery (ASAPS) in Vancouver, BC. ASAPS ... surgeons focused on aesthetic body contouring and is ... (Photo: http://photos.prnewswire.com/prnh/20120504/NY98979 ) ...
...  The San Diego Chapter of the National Investor Relations ... Tips & Tactics for Establishing & Maintaining a Successful ... 2012 meeting. The panel of seasoned IR ... and share their unique perspectives on what makes an ...
Cached Biology Technology:New technique uses electrons to map nanoparticle atomic structures 2New technique uses electrons to map nanoparticle atomic structures 3New HydraSolve™ Lipoplasty System launched at American Society of Aesthetic Plastic Surgery Meeting 2New HydraSolve™ Lipoplasty System launched at American Society of Aesthetic Plastic Surgery Meeting 3New HydraSolve™ Lipoplasty System launched at American Society of Aesthetic Plastic Surgery Meeting 4NIRI San Diego May Meeting: Seasoned IROs Discuss "Tips & Tactics for Establishing and Maintaining A Successful Investor Relations Program" 2NIRI San Diego May Meeting: Seasoned IROs Discuss "Tips & Tactics for Establishing and Maintaining A Successful Investor Relations Program" 3
... Clear yellow solution. ... 0.01% thimerosal, and other ... filtered. Diluent formulated to ... peroxidase antibody conjugates at ...
... WinMelt software is used ... of any DNA sequence up ... in optimizing placement of primers ... detection by denaturing gradient gel ...
... and PowerPac basic power supply are used for ... agarose gels. The Mini-Sub cell GT cell includes ... a 7 x 7 cm UV-transparent gel tray ... 1.5 mm fixed-height combs (8- and 15-well). The ...
... cell and PowerPac basic power supply are used ... in agarose gels. The mini format cell includes ... and leveling bubble. The PowerPac basic power supply, ... V, 4-400 mA, and 75 W. Power cord ...
Biology Products: