Navigation Links
Virologists make major step towards understanding the process of HIV infection

A working group of virologists headed by Professor Hans-Georg Kräusslich at Heidelberg University Hospitals, jointly with Professor Hanswalter Zentgraf, Division of Applied Tumor Virology of the Deutsches Krebsforschungszentrum (German Cancer Research Center, DKFZ), have been the first to label Human Immunodeficiency Viruses (HIV) for visual investigations without inhibiting the functional characteristics of the virus.

The labeling permits scientists to observe the behavior of the virus when it enters a host cell, during replication and when it leaves a cell. This is a major step towards understanding the process of HIV infection.

Modern imaging technologies facilitate real-time observation of virus-cell interactions. Many of these investigation methods require labeling of the object of interest, such as by introducing the genetic code of green fluorescent protein (GFP) into its genetic information. There the marker protein will be produced by the cellular machinery and appended to the desired site.

To observe the interaction of HIV with the host cell, there had also been attempts to label the virus with GFP. However, the genetic modifications impaired the formation of virus particles or their infectiousness, thus limiting the value of results obtained. A team of Heidelberg researchers of the university hospitals?Virology Section and the DKFZ have now found an area within the structure molecule of the viral capsid that tolerates the substantial extension by GFP. Although the insertion of the GFP molecule enlarges the HIV structure protein by about one half, infectious viruses continue to be generated. Using electron microscopy, Zentgraf’s team was able to show that appearance and shape of the virus particles thus created cannot be distinguished from normal HIV. By simultaneous production of GFP-extended and normal structure protein, PD Dr. Barbara Müller was able to produce, under a fluorescence microscope, clearly visible HIVs with several th
'"/>

Source:Deutsches Krebsforschungszentrum (German Cancer Research Center, DKFZ)


Page: 1 2

Related biology news :

1. Jefferson Virologists Coax HIV Out of Hiding
2. Columbia research lifts major hurdle to gene therapy for cancer
3. Researchers uncover sequence of major rice pathogen
4. Emergence of cancer as major cause of childhood death in developing countries is not being adequately addressed
5. Biochemists report discovery of structure of major piece of telomerase; implications for cancer
6. Biota makes major antiviral discovery
7. New gene scanning technology marks a major advance in disease research
8. LIAI scientists make major finding on potential smallpox treatment
9. MicroRNAs have shaped the evolution of the majority of mammalian genes
10. MIT: Oceans are a major gene swap-meet for plankton
11. U-M scientists identify major psoriasis susceptibility gene
Post Your Comments:
(Date:3/20/2015)... , Mar. 19, 2015 Research and Markets ... "Hand Geometry - Global Strategic Business Report" report ... markets for Hand Geometry in US$ Thousands. The report provides ... , Japan , Europe ... , and Rest of World. Annual estimates ...
(Date:3/19/2015)... , March 19, 2015  NXT-ID, Inc. (NASDAQ: ... company focused on the growing mobile commerce market, announces its ... a news clip that aired this week on ... . In a segment "The Next Great Thing", ... ,a new way to pay, and ,a really big breakthrough ...
(Date:3/17/2015)... March 17, 2015 Emotient, the leader ... today announced general availability of Emotient Analytics ... the analysis of facial expressions. The system analyzes ... content, products and services. It delivers audience response ... sentiment - as derived from facial evidence of ...
Breaking Biology News(10 mins):Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 2Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 4NXT-ID's Wocket Smart Wallet Featured on Fox News Segment, "The Next Great Thing" 2NXT-ID's Wocket Smart Wallet Featured on Fox News Segment, "The Next Great Thing" 3New Emotient Web Service Measures Audience Response Using On-Demand Facial Expression Analysis 2New Emotient Web Service Measures Audience Response Using On-Demand Facial Expression Analysis 3New Emotient Web Service Measures Audience Response Using On-Demand Facial Expression Analysis 4
... NT APRIL 2010 High in the Mackenzie Mountains, ... being revealed as warming temperatures melt patches of ice that ... Tom Andrews, an archaeologist with the Prince of Wales Northern ... Polar Year Ice Patch Study, is amazed at the implements ...
... April 26, 2010 Energy in, energy out, it,s the ... more than two thirds of Americans classified as overweight or ... Journal of Nutrition Education and Behavior examines how ... loss programs. Researchers at the University of Kentucky and ...
... 26 BIO-key International, Inc. (OTC Bulletin Board: BKYI), a leader ... (OK) Sheriff,s Office successful deployment of an FBI-compliant advanced authentication solution for ... authentication. , , , ... http://www.newscom.com/cgi-bin/prnh/20050509/BIOKEYLOGO ) , , , ...
Cached Biology News:Ancient artifacts reveals as northern ice patches melt 2What's motivation got to do with weight loss? 2BIO-key(R) Delivers FBI-Compliant 2 Factor Authentication Solution for First Responders 2BIO-key(R) Delivers FBI-Compliant 2 Factor Authentication Solution for First Responders 3BIO-key(R) Delivers FBI-Compliant 2 Factor Authentication Solution for First Responders 4
(Date:4/23/2015)... 23, 2015 Proove Biosciences ... Medicine, is excited to announce the launch of ... This profile assesses 62 genetic variations to predict ... for commonly prescribed non-opioid pain medications, namely ibuprofen ... alprazolam (brand name Xanax) and acetaminophen (brand name ...
(Date:4/23/2015)... , April 23, 2015 /PRNewswire/ - Crystal Research Associates, ... Informational Overview ® (EIO) on Aeterna Zentaris Inc. ... is available on Crystal Research Associates, website at ... Zentaris, website at www.aezsinc.com . Aeterna ... specialty biopharmaceutical company in oncology, endocrinology, and women,s health. ...
(Date:4/22/2015)... -- The Heinz Family Foundation today announced the winners ... will present unrestricted cash awards totaling $1.25 million ... determination in finding solutions to critical issues both ... include a prolific cartoonist whose quirky characters depict ... two former Marines who are re-engaging veterans in ...
(Date:4/22/2015)... , April 22, 2015   MPIRICA ... today announced $1.6M in Series A financing from ... The funding helps accelerate consumer and employer awareness ... covering surgeries at over 4,800 U.S. hospitals. ... you are probably very concerned about choosing the ...
Breaking Biology Technology:Proove Biosciences Launches World’s First Test to Predict Responders to Commonly Prescribed Non-Opioid Pain Medications 2Proove Biosciences Launches World’s First Test to Predict Responders to Commonly Prescribed Non-Opioid Pain Medications 3Crystal Research Associates, LLC Issues Executive Informational Overview (EIO) on Aeterna Zentaris Inc. 2Crystal Research Associates, LLC Issues Executive Informational Overview (EIO) on Aeterna Zentaris Inc. 3From a Scientist Who Develops Human Microlivers to a New Yorker Cartoonist Who Masterfully Depicts the Humor and Heartbreak of Everyday Life: The 20th Heinz Awards Honors Six Changemakers 2From a Scientist Who Develops Human Microlivers to a New Yorker Cartoonist Who Masterfully Depicts the Humor and Heartbreak of Everyday Life: The 20th Heinz Awards Honors Six Changemakers 3From a Scientist Who Develops Human Microlivers to a New Yorker Cartoonist Who Masterfully Depicts the Humor and Heartbreak of Everyday Life: The 20th Heinz Awards Honors Six Changemakers 4From a Scientist Who Develops Human Microlivers to a New Yorker Cartoonist Who Masterfully Depicts the Humor and Heartbreak of Everyday Life: The 20th Heinz Awards Honors Six Changemakers 5From a Scientist Who Develops Human Microlivers to a New Yorker Cartoonist Who Masterfully Depicts the Humor and Heartbreak of Everyday Life: The 20th Heinz Awards Honors Six Changemakers 6MPIRICA Health Inc. Secures $1.6M to Promote FICO-Like Quality Scores for Surgeries 2
... Agreement Enables Spherix to Pursue All Pharmaceutical and Health-Oriented ... June 23 Spherix Incorporated (Nasdaq: SPEX ... a provider of technical and regulatory consulting services to ... it has terminated the 1996 license agreement pursuant to ...
... SAO PAULO, June 23 What does sugarcane have ... successful,30-year experience with sugarcane as a feedstock for ethanol. In ... replace a fossil fuel with,renewable energy has saved some 600 ... years for six billion trees to achieve the same,results. , ...
... , Continuing Supplies Will Be Used For NDA and ... Type 2 Diabetes , , BETHESDA, Md., June ... an innovator in biotechnology for diabetes therapy, and a ... supplement, biotechnology and pharmaceutical companies, today announced that it ...
Cached Biology Technology:Spherix Announces Termination of Arla License Agreement 2Spherix Announces Termination of Arla License Agreement 3Spherix Announces Termination of Arla License Agreement 4Sugarcane as a Key Element Against Global Warming to be Highlighted at Green Week in Brussels 2Sugarcane as a Key Element Against Global Warming to be Highlighted at Green Week in Brussels 3Sugarcane as a Key Element Against Global Warming to be Highlighted at Green Week in Brussels 4Spherix Announces Full-Scale Production of Pharmaceutical Tagatose 2Spherix Announces Full-Scale Production of Pharmaceutical Tagatose 3Spherix Announces Full-Scale Production of Pharmaceutical Tagatose 4
ID clarifier: With ethidium bromide (50 μg/ml)...
... Propidium Iodide (PI) is a fluorescent ... double-stranded nucleic acids. As a nuclear ... to dark red in color. The ... green or blue fluorochromes such as FITC, ...
... were cultured in DMEM and harvested at the ... keep the antigens in their native forms, cells ... arrayed on a 12-well (5 mm) adhesive coated ... enhance cellular attachment and to minimize background staining. ...
...
Biology Products: