Navigation Links
UW scientists report a new method to speed bird flu vaccine production

In the event of an influenza pandemic, the world's vaccine manufacturers will be in a race against time to forestall calamity. But now, thanks to a new technique to more efficiently produce the disarmed viruses that are the seed stock for making flu vaccine in large quantities, life-saving inoculations may be available more readily than before. The work is especially important as governments worldwide prepare for a predicted pandemic of avian influenza.

Writing this week (Oct. 31, 2005) in the online edition of the Proceedings of the National Academies of Science (PNAS), a team of researchers from the University of Wisconsin-Madison and the University of Tokyo report a new way to generate genetically altered influenza virus. The lab-made virus - whose genes are manipulated to disarm its virulent nature - can be seeded into chicken eggs to generate the vaccine used in inoculations, which prepare the human immune system to recognize and defeat the wild viruses that spread among humans in an epidemic or pandemic.

In their report, a team led by UW-Madison virologists Yoshihiro Kawaoka and Gabriele Neumann, describes an improved "reverse genetics" technique that makes it easier to make a seed virus in monkey kidney cells, which, like tiny factories, churn out millions of copies of the disarmed virus to be used to make vaccines.

In nature, viruses commandeer a cell's reproductive machinery to make new virus particles, which go on to infect other cells and make yet more virus particles. Vaccine makers use a monkey kidney cell line to make non-virulent viruses that serve as the raw material for vaccines.The technique reported by the Wisconsin team improves upon a previous reverse genetics method (developed by Kawaoka's group in 1999) by significantly reducing the number of plasmid vectors required to ferry viral genes into the monkey kidney cells used to produce the virus particles to make vaccines."Compared to other types of cells, which are not a pproved for vaccine production, it is not always easy to introduce plasmids into the monkey kidney cells, which are approved for such use," says Kawaoka, an influenza expert and a professor of pathobiological sciences in UW-Madison's School of Veterinary Medicine. Monkey kidney cells are used routinely for generation of seed strains for vaccine production because they are not known to carry any unknown infectious agents and do not cause tumors.

According to Kawaoka, "application of the new system may be especially advantageous in situations of outbreaks of highly pathogenic avian influenza viruses."

When a new strain of highly virulent influenza emerges to infect humans, vaccine makers must tailor their vaccines to match it because, genetically, the virus is always different. The process is a race against time and can take months depending on how quickly new strains are identified, genetically disarmed and subsequently generated in the lab for use to make vaccines in large quantities. The new technique promises to ensure ready generation of seed strains for the production of vaccines required to blunt the spread of influenza. In the event of an outbreak of especially virulent strains of influenza, such as the H5N1 or "bird flu" viruses now being monitored by scientists, any efficiency in the manufacture of vaccines will be important.

The method devised by Kawaoka and his colleagues reduces the number of plasmids required to introduce viral genes into the monkey kidney cell lines used to mass produce the deactivated virus for use in vaccine manufacture. "By reducing the number of plasmids, we increase the efficiency of virus production," Kawaoka explains.

In addition to Kawaoka, the new PNAS report was authored by Neumann of the UW-Madison School of Veterinary Medicine, Ken Fujii of the University of Tokyo's Institute of Medical Sciences, and Yoichiro Kino of Japan's Chemo-Sero Therapeutic Research Institute. The work was funded by gr ants from the U.S. National Institutes of Health, the Ministries of Education, Culture, Sports, Science and Technology of Japan, and by the Core Research for Evolutional Science and Technology.


'"/>

Source:University of Wisconsin-Madison


Related biology news :

1. Wisconsin scientists grow critical nerve cells
2. UCSB scientists probe sea floor venting to gain understanding of early life on Earth
3. UAB scientists discover the origin of a mysterious physical force
4. Fox Chase Cancer Center scientists identify immune-system mutation
5. Weizmann Institute scientists develop a new approach for directing treatment to metastasized prostate cancer in the bones.
6. U-M scientists find genes that control growth of common skin cancer
7. UCLA scientists transform HIV into cancer-seeking missile
8. RNA project to create language for scientists worldwide
9. Carnegie Mellon scientists develop tool that uses MRI to visualize gene expression in living animals
10. To control germs, scientists deploy tiny agents provocateurs
11. Leprosy microbes lead scientists to immune discovery
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/6/2017)... 6, 2017 Forecasts by Product ... Readers, by End-Use (Transportation & Logistics, Government & Public ... & Fossil Generation Facility, Nuclear Power), Industrial, Retail, Business ... Are you looking for a definitive report on ... ...
(Date:4/5/2017)... April 4, 2017 KEY FINDINGS ... expand at a CAGR of 25.76% during the forecast ... the primary factor for the growth of the stem ... https://www.reportbuyer.com/product/4807905/ MARKET INSIGHTS The global stem cell ... application, and geography. The stem cell market of the ...
(Date:3/30/2017)... March 30, 2017  On April 6-7, 2017, Sequencing.com ... Genome hackathon at Microsoft,s headquarters in ... will focus on developing health and wellness apps that ... Hack the Genome is the first hackathon for ... world,s largest companies in the genomics, tech and health ...
Breaking Biology News(10 mins):
(Date:9/14/2017)... ... , ... Boston Strategic Partners, Inc. (BSP), a life-sciences and ... Research (HEOR) and ‘big data’ to provide in-depth analysis of pneumonia patients characteristics, ... trillion with nearly 1/3 spent on hospitalizations. BSP has access to real-world data ...
(Date:9/14/2017)... ... September 14, 2017 , ... ... analytics solutions, today announced that its Anzo Smart Data Lake has been named ... includes technologies and solutions that help organizations succeed in surpassing their knowledge management ...
(Date:9/12/2017)... ... September 12, 2017 , ... Avomeen.com, ... product testing and development companies will be exhibiting at the 16th annual Contract ... Contract Pharma is an educational conference for pharma and bio-pharma professionals ...
(Date:9/12/2017)... ... September 12, 2017 , ... September ... announce that Jeremy Nicholson, Head of the Department of Surgery and Cancer and ... 2018 Wallace H. Coulter Lecture. His presentation, “Analytical Science in Precision Medicine: ...
Breaking Biology Technology: